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Erschienen in: Obesity Surgery 6/2021

18.03.2021 | Original Contributions

The Impact of Gastric Bypass on Telomere Length and Shelterin Complex Gene Expression: 6 Months Prospective Study

verfasst von: Caroline Rossi Welendorf, Carolina Ferreira Nicoletti, Natália Yumi Noronha, Flávia Campos Ferreira, Letícia Santana Wolf, Marcela Augusta de Souza Pinhel, Vitor Caressato Pinhanelli, Cristiana Cortes de Oliveira, Bruno Affonso Parenti de Oliveira, Luzania dos Santos Martins, Wilson Salgado Junior, Carla Barbosa Nonino

Erschienen in: Obesity Surgery | Ausgabe 6/2021

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Abstract

Background

Telomeres are structures located at the ends of chromosomes associated with a protein complex, known as the shelterin complex. In individuals with obesity, excess adipose tissue plays a key role in inducing a chronic and systemic inflammatory state, which can cause TL shortening. In this context, bariatric surgery is one of the most effective treatment modalities in improving metabolic control.

Aim

Therefore, the present study aimed to evaluate how a short postoperative period of gastric bypass affects TL and expression of POT1, TRF1 and TRF2 genes.

Methods

Forty-eight women submitted to RYGB were evaluated before and after 6 months of the surgical procedure. Anthropometric measures of body weight and height (BMI), abdominal circumference (AC), body composition, food intake and blood collection for biochemical evaluation, TL analysis (DNA), and gene expression (RNA) were collected at each moment.

Results

There was a reduction of weight, BMI, AC, FM and FFM as well as of glycemia, total cholesterol, LDL-cholesterol, and triglycerides after gastric bypass. No difference in energy intake and macronutrients consumption was observed. There was no significant change in TL, but there was a significant increase of POT1 and TRF1 gene expression after surgery, while TRF2 expression did not change.

Conclusions

Despite bariatric surgery is not capable of increasing telomere length in a short-term period, no reduction is observed; additionally, we found a correlation between serum triglycerides concentration and TL. The increase of POT1 and TRF1 gene expression may explain the maintenance of the TL after 6 months postoperative period.

Graphical Abstract

Literatur
1.
Zurück zum Zitat Gorenjak V, Akbar S, Stathopoulou MG, et al. The future of telomere length in personalized medicine. Front Biosci (Landmark Ed). 2018;23:1628–54.CrossRef Gorenjak V, Akbar S, Stathopoulou MG, et al. The future of telomere length in personalized medicine. Front Biosci (Landmark Ed). 2018;23:1628–54.CrossRef
2.
Zurück zum Zitat Mangge H, Renner W, Almer G, et al. Subcutaneous adipose tissue distribution and telomere length. Clin Chem Lab Med. 2019;57(9):1358–63.PubMedCrossRef Mangge H, Renner W, Almer G, et al. Subcutaneous adipose tissue distribution and telomere length. Clin Chem Lab Med. 2019;57(9):1358–63.PubMedCrossRef
3.
Zurück zum Zitat De Lange T. Shelterin: the protein complex that shapes and safeguards human telomeres. Genes Dev. 2005;19(18):2100–10.PubMedCrossRef De Lange T. Shelterin: the protein complex that shapes and safeguards human telomeres. Genes Dev. 2005;19(18):2100–10.PubMedCrossRef
4.
6.
8.
Zurück zum Zitat Wolkowitz OM, Mellon SH, Epel ES, et al. Leukocyte telomere length in major depression: correlations with chronicity, inflammation and oxidative stress--preliminary findings. PLoS One. 2011;6(3):e17837.PubMedPubMedCentralCrossRef Wolkowitz OM, Mellon SH, Epel ES, et al. Leukocyte telomere length in major depression: correlations with chronicity, inflammation and oxidative stress--preliminary findings. PLoS One. 2011;6(3):e17837.PubMedPubMedCentralCrossRef
9.
Zurück zum Zitat Shin YA. How does obesity and physical activity affect aging? Focused on telomere as a biomarker of aging. J Obes Metab Syndr. 2019;28(2):92–104.PubMedPubMedCentralCrossRef Shin YA. How does obesity and physical activity affect aging? Focused on telomere as a biomarker of aging. J Obes Metab Syndr. 2019;28(2):92–104.PubMedPubMedCentralCrossRef
10.
Zurück zum Zitat Von Zglinicki T. Oxidative stress shortens telomeres. Trends Biochem Sci. 2002;27(7):339–44.CrossRef Von Zglinicki T. Oxidative stress shortens telomeres. Trends Biochem Sci. 2002;27(7):339–44.CrossRef
12.
Zurück zum Zitat Weisberg SP, McCann D, Desai M, et al. Obesity is associated with macrophage accumulation in adipose tissue. J Clin Investig. 2003;112(12):1796–808.PubMedCrossRefPubMedCentral Weisberg SP, McCann D, Desai M, et al. Obesity is associated with macrophage accumulation in adipose tissue. J Clin Investig. 2003;112(12):1796–808.PubMedCrossRefPubMedCentral
13.
Zurück zum Zitat Tzanetakou IP, Katsilambros NL, Benetos A, et al. Is obesity linked to aging?: adipose tissue and the role of telomeres. Ageing Res Rev. 2012;11(2):220–9.PubMedCrossRef Tzanetakou IP, Katsilambros NL, Benetos A, et al. Is obesity linked to aging?: adipose tissue and the role of telomeres. Ageing Res Rev. 2012;11(2):220–9.PubMedCrossRef
14.
Zurück zum Zitat González-Guardia L, Yubero-Serrano EM, Rangel-Zuñiga O, et al. Influence of endothelial dysfunction on telomere length in subjects with metabolic syndrome: LIPGENE study. Age. 2014;36(4):9681.PubMedPubMedCentralCrossRef González-Guardia L, Yubero-Serrano EM, Rangel-Zuñiga O, et al. Influence of endothelial dysfunction on telomere length in subjects with metabolic syndrome: LIPGENE study. Age. 2014;36(4):9681.PubMedPubMedCentralCrossRef
15.
Zurück zum Zitat Le Roux CW, Heneghan HM. Bariatric Surgery for Obesity. Med Clin North Am. 2018;102(1):165–82.PubMedCrossRef Le Roux CW, Heneghan HM. Bariatric Surgery for Obesity. Med Clin North Am. 2018;102(1):165–82.PubMedCrossRef
16.
Zurück zum Zitat Carulli L, Anzivino C, Baldelli E, et al. Telomere length elongation after weight loss intervention in obese adults. Mol Genet Metab. 2016;118(2):138–42.PubMedCrossRef Carulli L, Anzivino C, Baldelli E, et al. Telomere length elongation after weight loss intervention in obese adults. Mol Genet Metab. 2016;118(2):138–42.PubMedCrossRef
17.
Zurück zum Zitat Hohensinner PJ, Kaun C, Ebenbauer B, et al. Reduction of premature aging markers after gastric bypass surgery in morbidly obese patients. Obes Surg. 2018;28(9):2804–10.PubMedPubMedCentralCrossRef Hohensinner PJ, Kaun C, Ebenbauer B, et al. Reduction of premature aging markers after gastric bypass surgery in morbidly obese patients. Obes Surg. 2018;28(9):2804–10.PubMedPubMedCentralCrossRef
18.
Zurück zum Zitat Formichi C, Cantara S, Ciuoli C, et al. Weight loss associated with bariatric surgery does not restore short telomere length of severe obese patients after 1 year. Obes Surg. 2014;24(12):2089–93.PubMedCrossRef Formichi C, Cantara S, Ciuoli C, et al. Weight loss associated with bariatric surgery does not restore short telomere length of severe obese patients after 1 year. Obes Surg. 2014;24(12):2089–93.PubMedCrossRef
20.
Zurück zum Zitat Jakicic JM, Wing RR, Lang W. Bioelectrical impedance analysis to assess body composition in obese adult women: the effect of ethnicity. Int J Obes Relat Metab Disord. 1998;22(3):243–9.PubMedCrossRef Jakicic JM, Wing RR, Lang W. Bioelectrical impedance analysis to assess body composition in obese adult women: the effect of ethnicity. Int J Obes Relat Metab Disord. 1998;22(3):243–9.PubMedCrossRef
21.
Zurück zum Zitat Nonino CB, Oliveira BAP, Chaves RCP, et al. Is there any change in phenotypic characteristics comparing 5 to 10 years of follow-up in obese patients undergoing roux-en-y gastric bypass? Arq Bras Cir Dig. 2019;32(3):e.1453.CrossRef Nonino CB, Oliveira BAP, Chaves RCP, et al. Is there any change in phenotypic characteristics comparing 5 to 10 years of follow-up in obese patients undergoing roux-en-y gastric bypass? Arq Bras Cir Dig. 2019;32(3):e.1453.CrossRef
22.
Zurück zum Zitat Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987;162(1):156–9.PubMedCrossRef Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987;162(1):156–9.PubMedCrossRef
23.
Zurück zum Zitat Cawthon RM. Telomere measurement by quantitative PCR. Nucleic Acids Res. 2002;30(10):e.47.CrossRef Cawthon RM. Telomere measurement by quantitative PCR. Nucleic Acids Res. 2002;30(10):e.47.CrossRef
24.
Zurück zum Zitat Scheinberg P, Cooper JN, Sloand EM, et al. Association of telomere length of peripheral blood leukocytes with hematopoietic relapse, malignant transformation, and survival in severe aplastic anemia. JAMA. 2010;304(12):1358–64.PubMedPubMedCentralCrossRef Scheinberg P, Cooper JN, Sloand EM, et al. Association of telomere length of peripheral blood leukocytes with hematopoietic relapse, malignant transformation, and survival in severe aplastic anemia. JAMA. 2010;304(12):1358–64.PubMedPubMedCentralCrossRef
26.
Zurück zum Zitat Fouquerel E, Lormand J, Bose A, et al. Oxidative guanine base damage regulates human telomerase activity. Nat Struct Mol Biol. 2016;23(12):1092–100.PubMedPubMedCentralCrossRef Fouquerel E, Lormand J, Bose A, et al. Oxidative guanine base damage regulates human telomerase activity. Nat Struct Mol Biol. 2016;23(12):1092–100.PubMedPubMedCentralCrossRef
27.
Zurück zum Zitat da Silva VR, Moreira EA, Wilhelm-Filho D, et al. Proinflammatory and oxidative stress markers in patients submitted to Roux-en-Y gastric bypass after 1 year of follow-up. Eur J Clin Nutr. 2012;68(8):891–9.CrossRef da Silva VR, Moreira EA, Wilhelm-Filho D, et al. Proinflammatory and oxidative stress markers in patients submitted to Roux-en-Y gastric bypass after 1 year of follow-up. Eur J Clin Nutr. 2012;68(8):891–9.CrossRef
28.
Zurück zum Zitat Melissas J, Mouzas J, Filis D, et al. The intragastric balloon - smoothing the path to bariatric surgery. Obes Surg. 2006;16(7):897–902.PubMedCrossRef Melissas J, Mouzas J, Filis D, et al. The intragastric balloon - smoothing the path to bariatric surgery. Obes Surg. 2006;16(7):897–902.PubMedCrossRef
29.
Zurück zum Zitat Laimer M, Melmer A, Lamina C, et al. Telomere length increase after weight loss induced by bariatric surgery: results from a 10 year prospective study. Int J Obes. 2016;40(5):773–8.CrossRef Laimer M, Melmer A, Lamina C, et al. Telomere length increase after weight loss induced by bariatric surgery: results from a 10 year prospective study. Int J Obes. 2016;40(5):773–8.CrossRef
30.
Zurück zum Zitat Welendorf CR, Nicoletti CF, Pinhel MAS, et al. Obesity, weight loss, and influence on telomere length: new insights for personalized nutrition. Nutrition. 2019;66:115–21.PubMedCrossRef Welendorf CR, Nicoletti CF, Pinhel MAS, et al. Obesity, weight loss, and influence on telomere length: new insights for personalized nutrition. Nutrition. 2019;66:115–21.PubMedCrossRef
31.
Zurück zum Zitat Lee M, Martin H, Firpo MADE. Inverse association between adiposity and telomere length: the Fels longitudinal study. Am J Hum Biol. 2011;23:100–6.PubMedPubMedCentralCrossRef Lee M, Martin H, Firpo MADE. Inverse association between adiposity and telomere length: the Fels longitudinal study. Am J Hum Biol. 2011;23:100–6.PubMedPubMedCentralCrossRef
32.
Zurück zum Zitat Chen S, Yeh F, Lin J, et al. Short leukocyte telomere length is associated with obesity in American Indians: the strong heart family study. Aging. 2014;6:380–9.PubMedPubMedCentralCrossRef Chen S, Yeh F, Lin J, et al. Short leukocyte telomere length is associated with obesity in American Indians: the strong heart family study. Aging. 2014;6:380–9.PubMedPubMedCentralCrossRef
33.
Zurück zum Zitat Batsis JA, Mackenzie TA, Vasquez E, et al. Association of adiposity, telomere length and mortality: data from the NHANES 1999-2002. Int J Obes. 2018;42:198–204.CrossRef Batsis JA, Mackenzie TA, Vasquez E, et al. Association of adiposity, telomere length and mortality: data from the NHANES 1999-2002. Int J Obes. 2018;42:198–204.CrossRef
34.
Zurück zum Zitat Farzaneh-Far R, Lin J, Epel E, et al. Telomere length trajectory and its determinants in persons with coronary artery disease: longitudinal findings from the heart and soul study. PLoS One. 2010;5(1):e8612.PubMedPubMedCentralCrossRef Farzaneh-Far R, Lin J, Epel E, et al. Telomere length trajectory and its determinants in persons with coronary artery disease: longitudinal findings from the heart and soul study. PLoS One. 2010;5(1):e8612.PubMedPubMedCentralCrossRef
35.
Zurück zum Zitat Révész D, Milaneschi Y, Verhoeven JE, et al. Telomere length as a marker of cellular aging is associated with prevalence and progression of metabolic syndrome. Obstet Gynecol Surv. 2015;70:181–2.CrossRef Révész D, Milaneschi Y, Verhoeven JE, et al. Telomere length as a marker of cellular aging is associated with prevalence and progression of metabolic syndrome. Obstet Gynecol Surv. 2015;70:181–2.CrossRef
36.
Zurück zum Zitat Peng H, Mete M, Desale S, et al. Leukocyte telomere length and ideal cardiovascular health in American Indians: the Strong Heart Family Study. Eur J Epidemiol. 2017;32:67–75.PubMedCrossRef Peng H, Mete M, Desale S, et al. Leukocyte telomere length and ideal cardiovascular health in American Indians: the Strong Heart Family Study. Eur J Epidemiol. 2017;32:67–75.PubMedCrossRef
37.
Zurück zum Zitat Van Steensel B, De Lange T. Control of telomere length by the human telomeric protein TRF1. Nature. 1997;385(6618):740–3.PubMedCrossRef Van Steensel B, De Lange T. Control of telomere length by the human telomeric protein TRF1. Nature. 1997;385(6618):740–3.PubMedCrossRef
38.
Zurück zum Zitat Takai H, Jenkinson E, Kabir S, et al. A POT1 mutation implicates defective telomere end fill-in and telomere truncations in Coats plus. Genes Dev. 2016;30(7):812–26.PubMedPubMedCentralCrossRef Takai H, Jenkinson E, Kabir S, et al. A POT1 mutation implicates defective telomere end fill-in and telomere truncations in Coats plus. Genes Dev. 2016;30(7):812–26.PubMedPubMedCentralCrossRef
39.
Zurück zum Zitat Harte AL, da Silva NF, Miller MA, et al. Telomere length attrition, a marker of biological senescence, is inversely correlated with triglycerides and cholesterol in South Asian males with type 2 diabetes mellitus. Exp Diabetes Res. 2012;2012:1–7.CrossRef Harte AL, da Silva NF, Miller MA, et al. Telomere length attrition, a marker of biological senescence, is inversely correlated with triglycerides and cholesterol in South Asian males with type 2 diabetes mellitus. Exp Diabetes Res. 2012;2012:1–7.CrossRef
40.
Zurück zum Zitat Aulinas A, Ramírez MJ, Barahona MJ, et al. Dyslipidemia and chronic inflammation markers are correlated with telomere length shortening in Cushing’s syndrome. PLoS One. 2015;10(3):e0120185.PubMedPubMedCentralCrossRef Aulinas A, Ramírez MJ, Barahona MJ, et al. Dyslipidemia and chronic inflammation markers are correlated with telomere length shortening in Cushing’s syndrome. PLoS One. 2015;10(3):e0120185.PubMedPubMedCentralCrossRef
41.
Zurück zum Zitat Strandberg TE, Saijonmaa O, Fyhrquist F, et al. Telomere length in old age and cholesterol across the life course. J Am Geriatr Soc. 2011;59(10):1979–081.PubMedCrossRef Strandberg TE, Saijonmaa O, Fyhrquist F, et al. Telomere length in old age and cholesterol across the life course. J Am Geriatr Soc. 2011;59(10):1979–081.PubMedCrossRef
42.
Zurück zum Zitat Dei Cas A, Spigoni V, Franzini L, et al. Lower endothelial progenitor cell number, family history of cardiovascular disease and reduced HDL-cholesterol levels are associated with shorter leukocyte telomere length in healthy young adults. Nutr Metab Cardiovasc Dis. 2013;23(3):272–8.PubMedCrossRef Dei Cas A, Spigoni V, Franzini L, et al. Lower endothelial progenitor cell number, family history of cardiovascular disease and reduced HDL-cholesterol levels are associated with shorter leukocyte telomere length in healthy young adults. Nutr Metab Cardiovasc Dis. 2013;23(3):272–8.PubMedCrossRef
43.
Zurück zum Zitat Satoh M, Ishikawa Y, Takahashi Y, et al. Association between oxidative DNA damage and telomere shortening in circulating endothelial progenitor cells obtained from metabolic syndrome patients with coronary artery disease. Atherosclerosis. 2008;198(2):347–53.PubMedCrossRef Satoh M, Ishikawa Y, Takahashi Y, et al. Association between oxidative DNA damage and telomere shortening in circulating endothelial progenitor cells obtained from metabolic syndrome patients with coronary artery disease. Atherosclerosis. 2008;198(2):347–53.PubMedCrossRef
44.
Zurück zum Zitat Cunha FM, Oliveira J, Preto J, et al. The effect of bariatric surgery type on lipid profile: an age, sex, body mass index and excess weight loss matched study. Obes Surg. 2016;26(5):1047.CrossRef Cunha FM, Oliveira J, Preto J, et al. The effect of bariatric surgery type on lipid profile: an age, sex, body mass index and excess weight loss matched study. Obes Surg. 2016;26(5):1047.CrossRef
46.
Zurück zum Zitat Epel ES. Psychological and metabolic stress: a recipe for accelerated cellular aging? Hormones (Athens). 2009;8(1):7–22.PubMedCrossRef Epel ES. Psychological and metabolic stress: a recipe for accelerated cellular aging? Hormones (Athens). 2009;8(1):7–22.PubMedCrossRef
Metadaten
Titel
The Impact of Gastric Bypass on Telomere Length and Shelterin Complex Gene Expression: 6 Months Prospective Study
verfasst von
Caroline Rossi Welendorf
Carolina Ferreira Nicoletti
Natália Yumi Noronha
Flávia Campos Ferreira
Letícia Santana Wolf
Marcela Augusta de Souza Pinhel
Vitor Caressato Pinhanelli
Cristiana Cortes de Oliveira
Bruno Affonso Parenti de Oliveira
Luzania dos Santos Martins
Wilson Salgado Junior
Carla Barbosa Nonino
Publikationsdatum
18.03.2021
Verlag
Springer US
Erschienen in
Obesity Surgery / Ausgabe 6/2021
Print ISSN: 0960-8923
Elektronische ISSN: 1708-0428
DOI
https://doi.org/10.1007/s11695-021-05299-9

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