Background
Kawasaki disease (KD) involves diffuse, systemic vasculitis of unknown etiology and pathogenesis, and predominantly affects infants and children [
1‐
3]. The incidence of KD has steadily increased since it was first reported, and more than 12,000 people are diagnosed with KD each year in Japan. The well-known sequelae of KD include coronary aneurysms, which occur in approximately 5% of the KD patients [
4]. With time, an aneurysm may cause stenotic lesions or ischemic heart disease, even in children [
5‐
7].
Intravascular immunoglobulin (IVIG) therapy can greatly decrease the chances of complications of coronary arterial lesions (CALs), though 10–15% of the patients are refractory to IVIG. In these cases, the incidence of CALs tends to be much higher.
Since KD was first described by Kawasaki in 1967, many clinical, histopathological and or molecular biological studies have been performed to investigate the pathophysiology of the vascular involvement of KD; however, the underlying mechanism remains unclear [
2,
3,
8,
9]. Coronary arteritis in KD is considered to begin with edematous changes in the media, developing into inflammatory changes in the intima and adventitia, which finally evolve into the panvasculitis observed during autopsies [
8].
In contrast, recent studies using various animal models of KD like vasculitis have indicated that the inflammatory changes in the adventitia occur prior to the changes in the intima [
10‐
12]. In 1975, Onouchi et al. suggested that the vasa vasorum, which supplies nutrition to the walls of blood vessels, might contribute to the progression of vasculitis in KD, beginning with severe tissue destruction at the vasa vasorum in the adventitia [
9]. Although there have not been detailed pathological studies on the significance of the vasa vasorum in KD, recent studies using the advanced imaging analyses have shown that the abnormal proliferation of the vasa vasorum was associated with plaque formation and the destabilization of lesions during the development of arterial sclerosis. In chronic inflammatory diseases such as arterial sclerosis, the abnormal proliferation of the vasa vasorum may provide an infiltrative route for inflammatory cells from the adventitia, and this accumulation of inflammatory cells promotes the inflammation [
13‐
15]. Thus, we hypothesized that the vasa vasorum might serve as the initiator of vasculitis in KD.
Various animal models have been developed to investigate KD vasculitis. In 1979, Murata and Naoe et al. first reported that the
Candida albicans derived substance (CADS) isolated from the feces of patients with KD causes coronary arteritis as seen in KD [
16]. Subsequent studies revealed that the water-soluble extracellular polysaccharide fraction (CAWS) obtained from the culture supernatant of
Candida albicans resulted in a stronger but similar vasculitis that occurred more frequently than that following exposure to CADS. Therefore, CAWS-induced vasculitis has become widely adopted as a universal animal model for KD like vasculitis [
17‐
25].
The purpose of this study was to delineate the involvement of the vasa vasorum in the development of KD vasculitis by examining its proliferation and distribution using histopathological or micro-computed tomography (CT) and scanning electron microscope (SEM) studies. KD is associated with a low mortality rate, and due to the low availability of autopsied hearts, we used the CAWS animal model for this purpose.
Methods
Animals
All experimental procedures were approved by the Committee for Animal Research, Kyoto Prefectural University of Medicine. A total of 24 four-week-old male C57BL/6N mice were obtained from SHIMIZU Laboratory Supplies Co., Ltd. (Kyoto, Japan) and were randomly divided into six experimental groups: control and model mice evaluated at one week, two weeks and three weeks after the administration of CAWS.
CAWS was kind gift from Tokyo University of Pharmacy and Life Science and 0.2 mL (20 mg/mL) was administered intraperitoneally to the model mice daily for five consecutive days starting at the age of five weeks.
Histopathology
After heparin was administered, anesthesia was induced with a fatal dose of pentobarbital sodium. The chest was then opened, and the abdominal aorta was cannulated and infused with saline until the venous effluent was free of blood. Sections of the heart at the level of the aortic valves were obtained.
Tissue specimens were fixed in 20% formalin, embedded in paraffin and sectioned at 4 μm-thick slices. First, hematoxylin and eosin (HE)- and elastic van Gieson (EVG)-stained sections were examined by light microscopy.
Next, immunohistochemical studies for CD3 and MPO were performed to detect the type of inflammatory cells. Some sections were deparaffinized in xylene and ethanol rinses. Only sections for CD3 were activated by boiling for 5 min in a citrate buffer (0.01 M) at pH 9.0 in a microwave. No pretreatment was needed for MPO. After blocking of non- specific binding, the sections were incubated at 4 °C overnight in a humidified slide chamber with polyclonal antibodies (Dako Cytomation) for CD3 (dilution 1:400) and MPO (dilution 1:600). After incubation with the corresponding secondary antibody for 30 minutes, the antibody binding was revealed using H2O2 and diaminobenzidine. Counterstaining was performed with hematoxylin, and sections were examined by light microscopy.
Immunofluorescent studies were also performed using Biotin-conjugated Isolectin IB4 (Molecular Probes, Inc.). Some sections were deparaffinized in xylene and ethanol rinses, and activated by boiling for 5 min in a citrate buffer (0.01 M) at pH 6.0 in a microwave. The sections were incubated at 4°C overnight in a humidified slide chamber with lectin diluted at 1:200. The stained specimens were observed using a confocal laser scanning microscope (LSM510 ver. 4.0; Carl Zeiss CO., Ltd., Oberkochen, Germany).
SEM
To examine the types of vessels, we investigated the adventitia of the micro- vessels using SEM [
26]. After administering lethal anesthesia with pentobarbital sodium, a blue synthetic resin (Mercox®, DIC, Tokyo) was injected through a 22 Ga plastic needle inserted into the left ventricle. The sample was maintained at room temperature until the liquid resin was completely polymerized. The heart, aorta and perivascular soft tissues were then harvested
en bloc. The soft tissues were dissolved by incubation in 20% potassium hydroxide solution at 50°C for three days. To completely dissolve the fat tissues, the samples were incubated with proteinase K in buffer ATL for one day. Then, the samples were washed in 0.5% Nonidet P-40. The microvascular casts were mounted on stubs, coated with osmium and examined using SEM (JSM-6320 F; JEOL, Tokyo).
Micro CT
After lethal anesthesia with pentobarbital sodium was induced, the thorax was opened. The abdominal aorta was cannulated and infused with heparinized saline until the venous effluent was free of blood. Next, the OMNIPAQUE 240 contrast agent was infiltrated from the abdominal aorta at a steady rate.
The aorta and coronary arteries were scanned
en bloc by a micro CT system (Rigaku, Tokyo, Japan) [
27‐
29]. The resulting three-dimensional images were displayed using the i-view software program (Morita, Kyoto, Japan). The number of pixels used to reconstruct the images was 500 × 500 × 500, and the time required for filming and reconstruction was two minutes. It was also possible to examine a three-dimensional tomographic image simultaneously. The pixel size was 10 μm, and the filming span was 5 mm × 5 mm.
Discussion
Kawasaki disease (KD) is characterized by systemic vasculitis with tissue edema at the initial phase of the disease. Although the inflammation is initially localized to the capillaries and microvessels, it eventually expands to medium-sized muscular arteries and veins. Our study found that the inflammation originated in the adventitia. The inflammation enhances the expression of growth factors and the resulting edema, leading to hypoxia due to the proliferation of the vasa vasorum (Figures
1,
2,
3). The proliferation of the vasa vasorum may act as a conduit for the entry of various growth factors [
35], cytokines and blood cells. The spread of the proliferated vasa vasorum to the media thus broadens the inflammation.
In general, the inflammatory cells infiltrate from the adventitia toward the external elastic lamina around the Valsalva sinus and aortic valves by approximately one week after the administration of CAWS. During the second week, significant inflammatory changes develop from the adventitia and media toward the intima, as shown in Figures
1,
2 and
3, especially around the right and left coronary sinuses. The inflammatory cells, predominantly consisting of polymorphonuclear neutrophil leukocytes, are observed along the elastic lamina. More than three weeks later, the inflammation expands circumferentially to include the non-coronary sinus, and the beginning of an abscess can be observed. Fibrinoid necrosis is detected in the internal elastic lamina, and the intima and the adventitia markedly thicken. Therefore, the basic structure of blood vessels is destroyed, and they become fragile.
In the very early phase of inflammation, although the inflammatory cells accumulate at the adventitia, the detachment of endothelial cells or a thrombus indicative of the damaged intima are not detected. This suggests that the vasculitis is initiated from the side of the adventitia [
36].
As the inflammatory cells infiltrate through the vasa vasorum, the neogenesis of more vasa vasorum occurs inward, and the inflammatory lesion expands along the elastic lamina.
While the arteries that form aneurysms are typically muscular, and occur along with the vasa vasorum (e.g., coronary artery, axillary artery, internal iliac artery), edema and cell infiltration are also seen at arterioles, venules, and microvessels. The pathophysiology of coronary aneurysms remains unclear, however, we hypothesize that both endothelial cell disorders and inflammation of the vasa vasorum are involved. If there is prolonged edema of the microvessels, the supply of oxygen to the coronary artery wall through the vasa vasorum will be compromised, and the coronary media may thus become ischemic. Hence, the coronary artery structure becomes very fragile [
37].
As previously noted, we believe that after the development of inflammation around micro arteries in the adventitia, the neovascularization diverges from the vasa vasorum and proliferates toward the media and the intima to supply blood, inflammatory cells, cytokines and chemokines in order to quell the inflammation.
The vasa vasorum have been of considerable interest to scientists and physicians for more than a century [
38,
39]. They nurture the outer component of the vessel wall, including the adventitia and outer one-third of the media, and the intima is supplied with oxygen from the lumen [
30]. Under normal conditions, the vasa vasorum run longitudinally along the long axis of the adventitia of the blood vessels (primary vasa vasorum), and in some places, flow into the adventitia and the media (second vasa vasorum).When any injury or inflammation of blood vessels occurs (e.g., arterial sclerosis), the vasa vasorum proliferate and penetrate into the adventitia [
13] and the media, thus buttressing the capillary network [
14].
Developments in diagnostic imaging have allowed the microvessels inside lesions to be detected if the neointima is thicker than 500 μm. Furthermore, any infiltration of microvessels from the adventitia into the vessels can now be visualized [
15].In inflammatory lesions, the permeability of microvessels can be seen in the acute phase as these microvessels expand or progress. Hypoxia and endothelial reactions are the major driving forces behind the progression of the vasa vasorum [
40]. Moreover, there is a disproportionate increase in growth factors for the endothelium of the vasa vasorum, and further development of the vasa vasorum causes the plasma to extravasate around the vasa vasorum.
Conclusions
In conclusion, we investigated the involvement of the vasa vasorum in the KD like vasculitis using a murine model induced by CAWS. We demonstrated that the vasa vasorum might serve as the initiator of vasculitis in this model. Initially, the inflammatory cells accumulated at the adventitia, and then diverged through neovascularization toward the media and the intima. Therefore, the vasa vasorum provides a critical route for the infiltration of inflammatory cells. The proliferation of the new vasa vasorum toward the media and the intima is an important criterion for both the expansion and the termination of the inflammation.
Competing interests
The authors declare no conflicts of interests.