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European Spine Journal
Erschienen in:

06.09.2024 | Original Article

The mechanical properties measurement could be affected by forceps: a technical note on sampling human annulus fibrosus

verfasst von: Tianchi Zhou, Bowei Xiao, Juying Huang, Tianhua Rong, Bingxuan Wu, Baoge Liu

Erschienen in: European Spine Journal | Ausgabe 11/2024

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Abstract

Purpose

To compare the mechanical properties of human annulus fibrosus obtained by forceps versus bistoury and observe whether the measurement could be affected by forceps sampling method.

Methods

In this study, the mechanical properties of the the extracellular matrix (ECM) of human annulus fibrosus, including elastic modulus and stiffness, were investigated using atomic force microscope (AFM). Tissue was obtained from patients during operation using a bistoury or nucleus pulposus forceps. Tissues obtained with the nucleus pulposus forceps were considered as the forceps group and those obtained with a bistoury were considered as the bistoury group.

Results

There was no significant difference observed between the forceps and bistoury group according to histological staining. The elastic modulus of the forceps group was 0.41 ± 0.08 MPa, and that of bistoury group was 0.53 ± 0.13 MPa, and the difference between the two groups was statistically significant (p < 0.05). The stiffness of the forceps group was 0.024 ± 0.003 N/m, and that of the bistoury group was 0.037 ± 0.003 N/m, and the difference between the two groups was statistically significant (p < 0.05).

Conclusion

The results indicate that the forceps sampling method has a substantial negative effect on the micromechanical properties of the ECM of the annulus fibrosus. Bistoury sampling method is recommended as the experimental subject for exploring the micromechanics mechanisms of cervical degenerative disease.
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Metadaten
Titel
The mechanical properties measurement could be affected by forceps: a technical note on sampling human annulus fibrosus
verfasst von
Tianchi Zhou
Bowei Xiao
Juying Huang
Tianhua Rong
Bingxuan Wu
Baoge Liu
Publikationsdatum
06.09.2024
Verlag
Springer Berlin Heidelberg
Erschienen in
European Spine Journal / Ausgabe 11/2024
Print ISSN: 0940-6719
Elektronische ISSN: 1432-0932
DOI
https://doi.org/10.1007/s00586-024-08438-9

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