The neuro-protective role of telomerase via TERT/TERF-2 in the acute phase of spinal cord injury
- 10.05.2023
- Original Article
Erschienen in:
- Verfasst von
- Dong-Gune Chang
- Jang-Woon Kim
- Hong Jin Kim
- Young-Hoon Kim
- Sang-Il Kim
- Kee-Yong Ha
- Erschienen in
- European Spine Journal | Ausgabe 7/2023
Abstract
Purpose
To investigate the interaction of telomerase activity and telomere length on neuro-protection or neuro-degeneration effects after spinal cord injury (SCI).
Methods
A contusive SCI model was developed using 56 Sprague–Dawley rats. Seven rats were allocated into acute injury phase groups (1, 3, 8, 24, and 48 h), and sub-acute and chronic injury phase groups (1, 2, and 4 weeks). Telomerase activity was assessed by telomerase reverse transcriptase (TERT) and telomeric repeat binding factor-2 (TERF-2). Differentiation of activated neural stem cells was investigated by co-expression of neuronal/glial cell markers. Apoptosis expression was also investigated by caspase-3, 8, and 9 using terminal deoxynucleotidyl transferase dUTP nick end labelling staining. Immunofluorescence staining and western blotting were performed for quantitative analyses.
Results
Expression of TERT increased gradually until 24 h post-injury, and was decreased following SCI (P < 0.05). TERF-2 also was increased following SCI until 24 h post-injury and then decreased with time (P < 0.05). Co-localization of TERT and TERF-2 was higher at 24 h post-injury. High expression of TERT was seen in neurons (Neu N Ab), however, expression of TERT was relatively lower in astrocytes and oligodendrocytes. Apoptosis analysis showed persistent high expression of caspases-3, -9, and -8 during the observation period.
Conclusions
Increased TERT and TERF-2 activity were noted 24 h post-injury in the acute phase of SCI with TERF-2 maintaining telomeric-repeat length. Our results suggest that increased activity of telomere maintenance may be related to neuro-protective mechanisms against subsequent apoptosis resulting from DNA damage after acute SCI.
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- Titel
- The neuro-protective role of telomerase via TERT/TERF-2 in the acute phase of spinal cord injury
- Verfasst von
-
Dong-Gune Chang
Jang-Woon Kim
Hong Jin Kim
Young-Hoon Kim
Sang-Il Kim
Kee-Yong Ha
- Publikationsdatum
- 10.05.2023
- Verlag
- Springer Berlin Heidelberg
- Erschienen in
-
European Spine Journal / Ausgabe 7/2023
Print ISSN: 0940-6719
Elektronische ISSN: 1432-0932 - DOI
- https://doi.org/10.1007/s00586-023-07561-3
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