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Erschienen in: Journal of Bone and Mineral Metabolism 5/2020

15.04.2020 | Original Article

The old sheep: a convenient and suitable model for senile osteopenia

verfasst von: Stefan Maenz, Olaf Brinkmann, Ines Hasenbein, Christina Braun, Elke Kunisch, Victoria Horbert, Francesca Gunnella, André Sachse, Sabine Bischoff, Harald Schubert, Klaus D. Jandt, Jörg Bossert, Dominik Driesch, Raimund W. Kinne, Matthias Bungartz

Erschienen in: Journal of Bone and Mineral Metabolism | Ausgabe 5/2020

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Abstract

Introduction

Existing osteoporosis models in sheep exhibit some disadvantages, e.g., challenging surgical procedures, serious ethical concerns, failure of reliable induction of substantial bone loss, or lack of comparability to the human condition. This study aimed to compare bone morphological and mechanical properties of old and young sheep, and to evaluate the suitability of the old sheep as a model for senile osteopenia.

Materials and methods

The lumbar vertebral body L3 of female merino sheep with two age ranges, i.e., old animals (6–10 years; n = 41) and young animals (2–4 years; n = 40), was analyzed concerning its morphological and mechanical properties by bone densitometry, quantitative histomorphometry, and biomechanical testing of the corticalis and/or central spongious region.

Results

In comparison with young sheep, old animals showed only marginally diminished bone mineral density of the vertebral bodies, but significantly decreased structural (bone volume, − 15.1%; ventral cortical thickness, − 11.8%; lateral cortical thickness, − 12.2%) and bone formation parameters (osteoid volume, osteoid surface, osteoid thickness, osteoblast surface, all − 100.0%), as well as significantly increased bone erosion (eroded surface, osteoclast surface). This resulted in numerically decreased biomechanical properties (compressive strength; − 6.4%).

Conclusion

Old sheep may represent a suitable model of senile osteopenia with markedly diminished bone structure and formation, and substantially augmented bone erosion. The underlying physiological aging concept reduces challenging surgical procedures and ethical concerns and, due to complex alteration of different facets of bone turnover, may be well representative of the human condition.
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Metadaten
Titel
The old sheep: a convenient and suitable model for senile osteopenia
verfasst von
Stefan Maenz
Olaf Brinkmann
Ines Hasenbein
Christina Braun
Elke Kunisch
Victoria Horbert
Francesca Gunnella
André Sachse
Sabine Bischoff
Harald Schubert
Klaus D. Jandt
Jörg Bossert
Dominik Driesch
Raimund W. Kinne
Matthias Bungartz
Publikationsdatum
15.04.2020
Verlag
Springer Singapore
Erschienen in
Journal of Bone and Mineral Metabolism / Ausgabe 5/2020
Print ISSN: 0914-8779
Elektronische ISSN: 1435-5604
DOI
https://doi.org/10.1007/s00774-020-01098-x

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