The online version of this article (doi:10.1186/1477-7827-10-110) contains supplementary material, which is available to authorized users.
The authors declare that they have no competing interests.
KG and RD have made substantial contributions to conception and design and were responsible for acquisition of data. PG and KG were responsible for analysis and interpretation of data. JK, MWB and AM have been revising the manuscript critically for important intellectual content. MWB and RD has given final approval of the version to be published. MWB was supervisor of the present study. All authors read and approved the final manuscript.
It has previously been shown that the viability of swine uteri can be maintained within the physiological range in an open perfusion model for up to 8 hours. The aim of this study was to assess medium- to long-term perfusion of swine uteri using a modified Krebs–Ringer bicarbonate buffer solution (KRBB) in the established open perfusion model.
In an experimental study at an infertility institute, 30 swine uteri were perfused: group 1: n = 11, KRBB; group 2: n = 8, modified KRBB with drainage of perfusate supernatant; group 3: n = 11, modified KRBB with drainage of perfusate every 2 h and substitution with fresh medium. Modified and conventional KRBB were compared with regard to survival and contraction parameters: intrauterine pressure (IUP), area under the curve (AUC), and frequency of contractions (F).
Modified KRBB showed significantly higher IUP, AUC, and F values than perfusion with conventional KRBB. In group 3, the organ survival time of up to 17 h, with a 98% rate of effective contraction time, differed significantly from group 1 (P < 0.001).
Using modified KRBB in combination with perfusate substitution improves the open model for perfusion of swine uteri with regard to survival time and quality of contraction parameters. This model can be used for medium- to long-term perfusion of swine uteri, allowing further metabolic ex vivo studies in a cost-effective way and with little logistic effort.
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Ohura H, Kondo T, Handa M, Saito R, Matsumura Y, Okada Y, Shimada K, Hirose M, Horikoshi A, Sugita M, et al: Functional and histopathologic studies of primate pulmonary allografts preserved for 24 hours with a form of modified extracellular solution. J Heart Lung Transplant. 1995, 14: 493-504. PubMed
Bulletti C, Jasonni VM, Tabanelli S, Gianaroli L, Ciotti PM, Ferraretti AP, Flamigni C: Early human pregnancy in vitro utilizing an artificially perfused uterus. Fertil Steril. 1988, 49: 991-996. PubMed
Bulletti C, Jasonni VM, Martinelli G, Govoni E, Tabanelli S, Ciotti PM, Flamigni C: A 48-hour preservation of an isolated human uterus: endometrial responses to sex steroids. Fertil Steril. 1987, 47: 122-129. PubMed
Richter O, Wardelmann E, Dombrowski F, Schneider C, Kiel R, Wilhelm K, Schmolling J, Kupka M, van der Ven H, Krebs D: Extracorporeal perfusion of the human uterus as an experimental model in gynaecology and reproductive medicine. Hum Reprod. 2000, 15: 1235-1240. 10.1093/humrep/15.6.1235. CrossRefPubMed
Kim SY: Extrakorporale Uterusperfusion zur Untersuchung von Uterotonika in der Geburtshilfe und Reproduktionsmedizin [doctoral dissertation]. 2006, Bonn: University of Bonn, Available at: http://hss.ulb.uni-bonn.de/2006/0687/0687.pdf
Bessems M, Doorschodt BM, van Vliet AK, van Gulik TM: Preservation of rat livers by cold storage: a comparison between the University of Wisconsin solution and Hypothermosol. Ann Transplant. 2004, 9: 35-37. PubMed
Collins GM, Wicomb WN: New organ preservation solutions. Kidney Int Suppl. 1992, 38: S197-202. PubMed
- The perfused swine uterus model: long-term perfusion
Matthias W Beckmann
- BioMed Central
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