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01.12.2012 | Methodology | Ausgabe 1/2012 Open Access

Reproductive Biology and Endocrinology 1/2012

The perfused swine uterus model: long-term perfusion

Zeitschrift:
Reproductive Biology and Endocrinology > Ausgabe 1/2012
Autoren:
Klaudija Geisler, Julian Künzel, Philipp Grundtner, Andreas Müller, Matthias W Beckmann, Ralf Dittrich
Wichtige Hinweise

Electronic supplementary material

The online version of this article (doi:10.​1186/​1477-7827-10-110) contains supplementary material, which is available to authorized users.

Competing interests

The authors declare that they have no competing interests.

Authors’ contributions

KG and RD have made substantial contributions to conception and design and were responsible for acquisition of data. PG and KG were responsible for analysis and interpretation of data. JK, MWB and AM have been revising the manuscript critically for important intellectual content. MWB and RD has given final approval of the version to be published. MWB was supervisor of the present study. All authors read and approved the final manuscript.

Abstract

Background

It has previously been shown that the viability of swine uteri can be maintained within the physiological range in an open perfusion model for up to 8 hours. The aim of this study was to assess medium- to long-term perfusion of swine uteri using a modified Krebs–Ringer bicarbonate buffer solution (KRBB) in the established open perfusion model.

Methods

In an experimental study at an infertility institute, 30 swine uteri were perfused: group 1: n = 11, KRBB; group 2: n = 8, modified KRBB with drainage of perfusate supernatant; group 3: n = 11, modified KRBB with drainage of perfusate every 2 h and substitution with fresh medium. Modified and conventional KRBB were compared with regard to survival and contraction parameters: intrauterine pressure (IUP), area under the curve (AUC), and frequency of contractions (F).

Results

Modified KRBB showed significantly higher IUP, AUC, and F values than perfusion with conventional KRBB. In group 3, the organ survival time of up to 17 h, with a 98% rate of effective contraction time, differed significantly from group 1 (P < 0.001).

Conclusions

Using modified KRBB in combination with perfusate substitution improves the open model for perfusion of swine uteri with regard to survival time and quality of contraction parameters. This model can be used for medium- to long-term perfusion of swine uteri, allowing further metabolic ex vivo studies in a cost-effective way and with little logistic effort.
Zusatzmaterial
Authors’ original file for figure 1
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Authors’ original file for figure 2
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Authors’ original file for figure 3
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Authors’ original file for figure 8
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Authors’ original file for figure 9
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Literatur
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