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16.08.2018 | Original Paper

The pivotal role of microRNA-21 in osteoclastogenesis inhibition by anthracycline glycoside aloin

verfasst von: Radha Madhyastha, Harishkumar Madhyastha, Yutthana Pengjam, Queen Intan Nurrahmah, Yuichi Nakajima, Masugi Maruyama

Erschienen in: Journal of Natural Medicines | Ausgabe 1/2019

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Abstract

Osteopenic disorders such as osteoporosis and rheumatoid arthritis are characterized by excessive bone resorption by osteoclasts relative to bone formation by osteoblasts. MicroRNAs are emerging as key players in bone remodeling, modulating the functions of both osteoblasts and osteoclasts. Among them, miR-21 is highly expressed in osteoclast precursors and is known to regulate genesis, differentiation, and apoptosis of osteoclasts. The pro-osteoclastogenic nature of miR-21 makes it a potential candidate as a therapeutic target to treat bone disorders. We had previously demonstrated that anthroglycoside aloin derived from Aloe vera was effective in promoting osteoblastogenesis and inhibiting osteoclastogenesis. The present study investigated the role of miR-21 in aloin’s inhibitory effect on osteoclast differentiation. Aloin effectively suppressed receptor activator of nuclear factor kappa-B (NFĸB) ligand (RankL)-induced miR-21 expression via repression of NFĸB activation. MiR-21 suppression resulted in upregulation of osteoclast suppressor programmed cell death protein 4 (PDCD4), and downregulation of osteoclast marker cathepsin K. Knockdown or gain-of-function studies revealed that miR-21 was pivotal to aloin’s inhibitory effect on osteoclastogenesis. This study also highlights the dynamic potential of aloin as a therapeutic agent to treat osteopenic disorders.

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Titel: The pivotal role of microRNA-21 in osteoclastogenesis inhibition by anthracycline glycoside aloin
verfasst von: Radha Madhyastha
Harishkumar Madhyastha
Yutthana Pengjam
Queen Intan Nurrahmah
Yuichi Nakajima
Masugi Maruyama
Publikationsdatum: 16.08.2018
Verlag: Springer Singapore
Erschienen in: Journal of Natural Medicines / Ausgabe 1/2019
Print ISSN: 1340-3443
Elektronische ISSN: 1861-0293
DOI: https://doi.org/10.1007/s11418-018-1237-3

Springer Medizin

Abstract

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