The authors declare that they have no competing interests.
DB organized the study design, performed statistical analysis and drafted the manuscript. BB participated in cystometric and histological studies. TA participated in design of the study and animal surgeries. JO performed all MRI procedure troubleshooting. MH analyzed all MRI images and participated in cystometric studies. JT helped design and interpret CT and MRI studies. KEA was involved in conception, design, coordination of the study and helped draft the manuscript. GJC was involved in conception, design, coordination of the study and helped draft the manuscript. All authors have read and approved the final manuscript.
Restoration of normal bladder volume and function (i.e., bioequivalent bladder) are observed within 8 weeks of performing subtotal cystectomy (STC; removal of ~70 % of the bladder) in 12-week old rats. For analysis of bladder function in rodents, terminal urodynamic approaches are largely utilized. In the current study, we investigated the potential for Computed Tomography (CT) and Magnetic Resonance Imaging (MRI) scans to noninvasively track restoration of structure and function following STC.
Twelve week old female Fisher F344 rats underwent STC and were scanned via CT and/or MRI 2, 4, 8, and 12 weeks post-STC, followed by urodynamic testing. After euthanasia, bladders were excised for histological processing.
MRI scans demonstrated an initial decline followed by a time-dependent increase to normal bladder wall thickness (BWT) by 8 weeks post-STC. Masson’s trichrome staining showed a lack of fibrosis post-STC, and also revealed that the percent of smooth muscle in the bladder wall at 2 and 4 weeks positively correlated with pre-operative baseline BWT. Moreover, increased BWT values before STC was predictive of improved bladder compliance at 2 and 4 weeks post-STC. Cystometric studies indicated that repeated MRI manipulation (i.e. bladder emptying) apparently had a negative impact on bladder capacity and compliance. A “window” of bladder volumes was identified 2 weeks post-STC via CT scanning that were commensurate with normal micturition pressures measured in the same animal 6 weeks later.
Taken together, the data indicate some limitations of “non-invasive” imaging to provide insight into bladder regeneration. Specifically, mechanical manipulation of the bladder during MRI appears to negatively impact the regenerative process per se, which highlights the importance of terminal cystometric studies.
Liang DS, Goss RJ. Regeneration of the bladder after subtotal cystectomy in rats. J Urol. 1963;89:427–30. PubMed
Baker R, Maxted WC, Dipasquale N. Regeneration of Transitional Epithelium of the Human Bladder after Total Surgical Excision for Recurrent, Multiple Bladder Cancer: Apparent Tumor Inhibition. J Urol. 1965;93:593–7. PubMed
Baker R, Tehan T, Kelly T. Regeneration of urinary bladder after subtotal resection for carcinoma. Am Surg. 1959;25(5):348–52. PubMed
Bohne AW, Urwiller KL. Experience with urinary bladder regeneration. J Urol. 1957;77(5):725–32. PubMed
Folsom AI, O’Brien HA, Caldwell GT. Subtotal Cystectomy in the treatment of Hunner Ulcer. J Urol. 1940;44:650.
Liang DS. Bladder regeneration following subtotal cystectomy. J Urol. 1962;88:503–5. PubMed
McCallum DC. Gangrene of the bladder with subsequent regrowth. J Urol. 1965;94(6):669–70. PubMed
Richardson EJ. Bladder regeneration case report and review of the literature. Minn Med. 1952;35(6):547–9. PubMed
Sisk IR, Neu VF. Regeneration of the Bladder. Trans Am Assn GU Surg. 1939;32:197.
Tucci P, Haralambidis G. Regeneration of the Bladder: Review of Literature and Case Report. J Urol. 1963;90:193–9. PubMed
Newport JP, Dusseault BN, Butler C, Pais VM, Jr. Gadolinium-enhanced computed tomography cystogram to diagnose bladder augment rupture in patients with iodine sensitivity. Urology. 2008; 71(5):984 e989-911.
Burmeister D, AbouShwareb T, D’Agostino Jr R, Andersson KE, Christ GJ. Impact of partial urethral obstruction on bladder function: time-dependent changes and functional correlates of altered expression of Ca(2)(+) signaling regulators. Am J Physiol Ren Physiol. 2012;302(12):F1517–28. CrossRef
- The potential utility of non-invasive imaging to monitor restoration of bladder structure and function following subtotal cystectomy (STC)
- BioMed Central
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