Background
Helicobacter pylori, a Gram-negative spiral bacterium, is one of the most common infections worldwide [
1‐
3]. It colonizes the stomach of humans, usually in infancy [
4], and has been implicated in a number of diseases including gastritis, gastroduodenal ulceration and gastric cancer [
3,
5‐
7]. Approximately 20 % of individuals infected with
H. pylori develop overt disease symptoms, the severity of which is extremely variable. Many groups have focused on identifying
H. pylori virulence factors that may contribute to disease severity and a number of candidates have been proposed, including the vacuolating cytotoxin VacA [
8,
9] and the cytotoxin-associated gene product CagA [
9‐
11]. However, disease progression appears to be multi-factorial in that it also depends on a number of host factors including the immune and inflammatory response [
12‐
15]. This includes the secretion of cytokines by the gastric epithelium [
16‐
18] or mucosal antigen presenting cells [
19], increased recruitment of cells such as neutrophils, a robust IgA response [
20,
21] and T cell polarization [
22‐
25]. Ultimately, this chronic, active inflammatory response contributes to tissue damage and the subsequent pathogenesis of gastroduodenal disease.
Despite the impressive advances to date, much remains to be learned about the host responses that regulate the magnitude of the inflammation and tissue damage. One mechanism that impacts inflammation involves phagocytes and their interaction and uptake of apoptotic cells, which attenuates phagocyte inflammatory responses, leading to termination of inflammation and initiation of tissue repair [
26,
27]. After encountering apoptotic cells, phagocytes produce less pro-inflammatory mediators and increase their expression of anti-inflammatory, pro-resolution factors such as IL-10 and TGF-β1 [
28‐
30]. The clearance of apoptotic cells is influenced by many soluble factors and receptor interactions. C1q, the initiating protein of the complement cascade, has been shown to be an important opsonin of apoptotic cells, enhancing their recognition and removal by phagocytes [
31‐
33]. The importance of C1q is underscored in the autoimmune disease, systemic lupus erythematosus (SLE) where patients have a deficiency in C1q, from which the pathology has been linked to failed clearance of apoptotic cells [
34].
In the present study, we examined the processes whereby gastric epithelial cells (AGS cells) are recognized by macrophages (THP-1 macrophages) in response to apoptosis induced by
H. pylori infection. The attenuation of inflammatory responses by phagocytes following co-culture with sterile and infected apoptotic epithelial cells was also compared. As the complement protein C1q is one of several factors involved in the clearance of apoptotic cells [
31,
32] and in light of recent reports of a direct role for C1q in modulation of macrophage inflammatory responses [
35‐
39], we investigated the role of C1q in the interaction of infected gastric epithelial cells with phagocytes as well as its role in modulation of phagocyte cytokine responses.
Methods
Bacterial culture, cell lines and reagents
Helicobacter pylori strain 26695 was maintained routinely on blood agar plates containing 5 % horse blood (BD Pharmingen, San Jose, CA) at 37 °C in 10 % CO
2. Prior to infection of cell cultures, bacteria were amplified in Brucella broth containing 10 % heat-inactivated FBS for 18 h. The AGS human gastric epithelial cell line and the THP-1 monocyte-like cell line were obtained from ATCC (Rockville, MD). The gastric epithelial cell line, AGS cells, were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) and THP-1 in RPMI 1640 (Gibco, NY), both containing 10 % heat-inactivated fetal bovine serum (FBS, Sigma, St. Louis, MO), at 37
oC in 5 % CO
2. THP-1 cells were differentiated into a macrophage-like phenotype by treating with 600 nM phorbol myristate acetate (PMA; Sigma, St Lois, MO) for 3 days. Apoptosis was induced in AGS cells by infection with
H. pylori as previously described [
19] or by treatment with 3 μM camptothecin (Sigma, St. Louis, MO) for 24 h which proved a non-infectious means to induce apoptosis. Stimulation of apoptosis was carried out in the presence of 10 % FBS (heat-inactivated) medium after which the cells were washed twice in PBS (300 ×
g; 5 min) before being resuspended in the appropriate media prior to macrophage co-culture. The concentration of camptothecin or
H. pylori used has previously been determined to be an optimal concentration to induce apoptosis in epithelial cells. Purified human C1q protein was purchased from Quidel (San Diego, CA).
Preparation of human monocyte-derived macrophages
Human monocytes were isolated from blood drawn from healthy volunteers using a well-established technique involving dextran sedimentation followed by Percoll gradient separation [
26]. Mononuclear cells were suspended in DMEM supplemented with 10 % autologous serum at 1 × 10
6 cells/ml, and then 1 ml of the suspension was added to individual wells on a 24-well plate. The plate was incubated for 1 h at 37 °C and 5 % CO
2, after which time non-adherent cells were removed by washing. Maturation of the mononuclear cells into macrophages was induced by culturing for 5–7 days in DMEM with 10 % autologous serum. All procedures using human blood were approved in advance by the Institutional Review Board of the University of Virginia and University of California, San Diego.
Evaluation of phagocyte-target interactions by microscopy
Mature THP-1-derived macrophages were gently scraped and seeded onto an 8-well chamber slide (NUNC, Naperville, IL) at a density of 3 × 104 and grown overnight. The same day, epithelial cells were infected with H. pylori strain 26695 at a MOI of 300:1 or treated with camptothecin (3 μM). The following day, the macrophages were incubated with 2.5 μg/ml of the cytoplasmic dye CMFDA (Molecular Probes, Eugene, OR) for 1 h at 37 °C and 5 % CO2. Epithelial cells were trypsinized and stained in a similar manner with 2.5 μg/ml of the dye SNARF® (Molecular Probes). Both dyes freely cross the cytoplasmic membrane, where they are modified by intracellular esterases to yield a fluorescent product, which cannot cross the cell membrane. Both sets of cells were washed three times, and then apoptotic or control AGS cells were added to individual wells of the chamber slide containing macrophages at a ratio of 5:1 in media containing normal FCS that was not heat inactivated. Results with FCS were comparable to using human serum (data not shown). Interactions were allowed to proceed for 1 h at 37 °C and 5 % CO2, after which time the reaction was stopped with PBS containing 0.01 % sodium azide. The slide was washed three times with PBS, fixed with 2 % paraformaldehyde for 40 min at 37 °C, and then the plastic wells and silicon adhesive were removed and discarded. Finally the slide was mounted with a cover slip and allowed to dry. Recognition and binding of apoptotic epithelial cells by and macrophages were assessed by counting using an Axioscope fluorescent microscope (Zeiss, Thornwood, NY). For each condition, four randomly selected fields in replicate slides were photographed and the total number of green fluorescent cells (macrophages) and red fluorescent cells (AGS) were counted. The number of macrophages with one, two or three or more AGS attached was recorded.
Evaluation of cytokine responses
THP-1 cells were differentiated into a macrophage-like phenotype by treating with 600 nM phorbol myristate acetate (PMA; Sigma, St Louis, MO) for 3 days in 48-well plates. Cell numbers in the wells were approximately 100,000 and were quantified prior to the cell co-culture assay by trypsinizing cells and counting in a heamocytometer. AGS cells were made apoptotic with either camptothecin treatment (3 μM) or with H. pylori infection (MOI 100) overnight in T75 cm flasks. AGS cells were approx. 80–90 % confluent before infection and cell numbers were counted from a spare, identically seeded flask to calculate the appropriate MOI for the infection. On the day of the co-culture, AGS cells were trypsinized, washed twice in PBS (300 × g for 5 min), counted, and resuspended in THP-1 media (RPMI + 10 % FBS+ 10 mM HEPES + pen strep) or X-vivo 10 media (+2 mM L-glut) at 3 times the THP-1 cell number per ml (phagocyte:target ratio 3:1). The supernatant was removed from the THP-1 macrophages and washed using PBS to remove serum traces before addition of 1 ml of the AGS cell suspension. Where appropriate, the THP-1 macrophages were pre-treated with 5 μg/ml cytochalasin D (Sigma, St Louis, MO) for 30 min prior to and throughout AGS co-culture. The co-culture was performed for 2 h at 37 °C after which, the AGS cells were washed away from the adherent THP-1 cell monolayer using cold PBS (1 ml; × 3 washes). Media was replaced +/− LPS 100 ng/ml (from Salmonella; Sigma, St Louis, MO) for 24 h after which, the supernatant was removed, centrifuged at 10,000 × g for 15 min and stored at −80 °C until used for specific ELISA for IL-6 or TNF-α (R&D Systems, Minneapolis, MN).
Polymerase chain reaction
Total RNA was extracted according to the manufacturer’s instructions using the RNeasy kit (Qiagen, Valencia, CA), and yield estimated spectrophotometrically. RNA was reverse transcribed using the Superscript kit (Invitrogen, Carlsbad, CA) random hexamer protocol as per the manufacturer’s instructions. Amplification of gC1qR, cC1qR, C1qRp, and CD91 mRNA was performed using TaqMan® pre-designed primers from Applied Biosystems (Foster City, CA). Primers were diluted 1:20 in TaqMan® Universal PCR Master Mix and water. Real-time PCR analysis was performed on a SmartCycler® (Cepheid, Sunnydale, CA) at 50 °C for 2 min, 95 °C for 10 min, 40 cycles of 95 °C for 15 s and 60 °C for 1 min.
Flow cytometry
Mouse anti-human phycoerythrin (PE)-conjugated monoclonal antibodies (mAb) to C1qRp (product #551087), FITC-conjugated mAb to CD91 (product #550496), purified anti-calreticulin (product #612137), a FITC-rat-anti-mIgG1 secondary antibody, (product #562026) were obtained from BD Biosciences (San Diego, CA). A purified mouse anti-human mAb to gC1qR was a generous gift from Dr. Young Hahn (University of Virginia). FITC-conjugated rabbit polyclonal anti-human C1q was purchased from Abcam (product # ab4223, Cambridge, MA). PE-mIgG2b,κ, (product #556656) PE-mIgG1,κ (product #551436), FITC- mIgG1,κ (product #554679), purified mIgG1,κ (product #349040) (BD Biosciences, San Diego CA) and FITC-Rabbit IgG (product #F0382), (Sigma, St Louis, MO) served as the isotype controls.
The assessment of apoptosis was performed using the Annexin V-FITC/Propidium iodide (PI) staining kit (R&D, Minneapolis, MN) as per manufacturer’s instructions. Cells were analyzed on a FACScan flow cytometer and analysed using FlowJo software (Treestar, Ashland, OR).
For complement receptor experiments, cells were suspended at 107/ml in PBS containing 1 % BSA and 0.01 % sodium azide. Antibodies were incubated with cells for 30 min on ice, washed twice in PBS containing 0.01 % sodium azide, and resuspended in 2 % PFA. When required, secondary labeling was performed as per primary labelling, following two washes in PBS with 0.01 % azide. Cells were analysed within 24 h on a BD FACSCalibur flow cytometer, and the data subsequently analysed using FlowJo software (Treestar, Ashland, OR).
C1q opsonization
Cells were seeded and allowed to adhere for 24 h prior to treatment with H. pylori (MOI 300:1), camptothecin (3 μM) or PBS (control cells). All treatments were performed in medium containing heat-inactivated serum. After 24 h of treatment, supernatants were collected, cells were washed once with PBS and trypsinzed. Trypsinized cells were added to supernatants and the cells then washed once in PBS. Cells were resuspended in either serum-free (SF) medium, medium containing 10 % non-heat-inactivated serum, or SF medium with 40 μg/ml C1q. After 1 h, cells were washed twice in PBS and resuspended at 107 per ml in PBS with 0.01 % sodium azide. FITC-Rabbit IgG or FITC-anti-C1q was added for 30 min on ice. Cells were subsequently washed twice in PBS with 0.01 % sodium azide and resuspended in 2 % PFA until flow cytometry was performed.
C1q cytokine studies
THP-1 cells were seeded at 0.25 × 106/ml in 48 well plates in the presence of 600 nM PMA for 3 days. On the day of the assay, the number of macrophages per well was approximately 100,000 and was verified using a hemocytometer. The cells were washed with PBS and re-suspended in X-vivo 10 medium (+2 mM L-glut). C1q protein was added to the cells at 0–80 μg/ml for 30 min prior to the addition of LPS (100 ng/ml) or H. pylori (MOI 100). Unstimulated controls were run in parallel. After 24 h of treatment, supernatants were collected, centrifuged at 10,000 ×g for 10 min and stored at −80 °C until assessed for cytokine (IL-6 and TNF-α) concentration using specific ELISAs (R&D Systems, Minneapolis, MN).
Statistics
Results are expressed as the mean ± SEM. Results were compared using two-tailed Student’s t-test, or one-way ANOVA with Tukey’s post-hoc correction when indicated. Statistical significance and p values are indicated in the figures.
Discussion
Engulfment of apoptotic cells is an important process for tissue remodelling and homeostasis. This response entails several steps including: recruitment of phagocytes; recognition of dead cells; binding of the corpse to a phagocyte; internalization of the target; target degradation and finally, modification of phagocyte cell function. Engulfment of apoptotic epithelial cells by macrophages or dendritic cells has been observed in the intestinal lamina propria [
47‐
49], the stomach [
50] and in the lung [
51]. Furthermore, epithelial cell apoptosis is highly inducible by infections [
41,
52‐
54] and in response to inflammation [
12,
55]. The engulfment of apoptotic cells does not only remove cells to prevent release of toxic cell contents, but it is now widely accepted that apoptotic cells promote an anti-inflammatory phenotype in the engulfing phagocyte, thereby limiting inflammation [
26,
27]. The current report shows that C1q opsonizes apoptotic AGS cells and also confers immunomodulatory properties to the phagocyte. Our model used the human THP-1 cell line that is differentiated into macrophage like cells using PMA. Previous studies using peripheral blood derived macrophages (PBDM) have been published by our group, which show comparable levels of AGS cell binding following
H. pylori infection [
56]. Furthermore, our previous work also show attenuation of PBDM cytokine responses following apoptotic AGS co-culture and are in concordance with what we observe with THP-1 macrophages. Thus, our use of the THP-1 cell line as a model macrophage is appropriate and representative of other human macrophage responses.
In healthy cells, nearly all of the phosphatidylserine (PS) is confined to the inner aspect of the plasma membrane and exposure of PS on the outer leaflet marks the cells as apoptotic. As discussed elsewhere [
57] many receptors and adaptor molecules have been implicated in the recognition and clearance of apoptotic cells including collectin receptors, calreticulin/CD91, Fcγ receptors, c-Mer, integrins such as αvβ3, scavenger receptors thrombospondin–CD36 and phosphatidylserine receptors like BAI1 and TIM4. Some of the recognition structures that bind to PS trigger a signalling response leading to engulfment (i.e., BAI1 [
58]) while others, such as Tim4, perform an accessory function that facilitates the PS-dependent uptake of the target [
59,
60]. It has been reported that complement proteins also bind to apoptotic cells, marking them for quick removal, and a large panel of different complement proteins are involved, including C1q, mannose-binding lectin and C3bi [
61,
62]. The present study revealed that the interactions between
H. pylori-infected cells and macrophages were mediated by a serum component and that C1q was sufficient to restore binding of
H. pylori-infected AGS cells to macrophages. Importantly, C1q also conferred an anti-inflammatory effect on macrophages stimulated with LPS or
H. pylori. The concentrations of C1q used in our experiments (40–80 μg/mL) are well within the normal range found in plasma (56–276 μg/mL). In relation to
H. pylori infection, Berstad and colleagues [
63,
64] have detected the terminal complement complex (TCC) in the gastric mucosa of subjects infected with
H. pylori showing that complement activation occurs during infection, which could regulate the interaction between APCs and apoptotic cells as well as regulate phagocyte reactivity to infection.
It has been reported that C1q can bind via its globular head to PS exposed on the surface membrane blebs of apoptotic cells [
31,
32,
46]. Indeed, induction of apoptosis in AGS cells with camptothecin allowed C1q to bind the target suggesting that the interaction was through the recognition of the dead cell and no bacterial component is required for the binding by C1q to occur. The fact that THP-1 cells expressed multiple receptors for C1q (gC1qR and C1qRp), supports the feasibility of the interaction. We analyzed the expression of peripheral blood derived macrophages (PBDMs) and showed these cells also express receptors for C1q. Using siRNA approaches to inhibit individual receptor expression were successful based on assays to measure gene expression. However, they failed to significantly block the C1q-mediated binding of the apoptotic cell (data not shown). This level of redundancy is common in binding and engulfment studies due to the presence of multiple receptors and mechanisms that phagocytes utilize to clear a wide array of different targets.
Several studies have demonstrated an effect of C1q on cytokine production by monocytes, macrophages and DCs during apoptotic cell uptake [
35‐
37]. The modulation of cytokines by C1q was variable depending on which cytokines and APC were being studied. In general, a reduction in pro-inflammatory cytokines, IL-1β and IL-1α were reported together with an increase in IL-10, IL-6 and MCP-1. In the current study, apoptotic AGS cells attenuated production of LPS-stimulated cytokines, IL-6 and TNF-α independent of target internalization or the presence of serum. Moreover, the attenuation of cytokine responses occurred regardless of the method used to induce apoptosis. It is important to note that infected cell co-cultures did not confer a net pro-inflammatory effect compared to macrophages alone. This is intriguing as there are reports of other bacterial species which can use apoptotic targets as a type of Trojan horse to invade phagocytes in order to disseminate to locations remote from the point of entry [
65,
66]. Current work by our lab and others has noted the redundancy of apoptotic cell internalization for attenuated macrophage responses [
56,
67]. In these studies, it has been shown that conditioned medium from apoptotic cells is insufficient to confer anti-inflammatory effects and that contact of the macrophage and apoptotic cells is required. Furthermore, we have previously shown that binding of PS residues is also not required for the attenuation response to occur [
56]. The receptors responsible for altering macrophage reactivity following apoptotic cell contact are yet to be elucidated.
No serum components were required for the apoptotic cell-induced attenuation of cytokine responses and additional C1q during co-culture did not enhance this effect (data not shown). However, C1q protein incubated directly with THP-1 macrophages in the absence of apoptotic cells did have a significant effect, demonstrating a dose-dependent effect of C1q on LPS-stimulated TNF-α. The attenuation of TNF-α was noteworthy as this suggests a role for C1q in control of host responses to infection. We observed a similar trend in attenuation of baseline (unstimulated) production of TNF-α which may also indicate that C1q could be involved in controlling the general stress response in macrophages induced, in this instance, by serum starvation. Although the mechanism of the attenuation of cytokines by C1q remains elusive in our model, a recent publication by Galvan et al. has identified activation of AMPK as a potential candidate pathway which is triggered by C1q to induce modulation of immune reactivity in murine macrophages [
68].
We show that
H. pylori-infected AGS gastric epithelial cells bind to macrophages and that this response is facilitated by the C1q component of complement. It has been confirmed that these apoptotic cells are internalized by macrophages [
56] although no additional enhancement of this process was observed with C1q in our hands (data not shown). As mentioned previously, the reported degree of receptor redundancy in the engulfment process makes it difficult to assess the importance of one factor [
69]. Other investigations have shown that the maturation and type of APC can influence the C1q enhanced uptake of apoptotic cells [
37]. They reported that monocytes but not monocyte-derived macrophages or DCs, enhanced their phagocytic capacity for apoptotic cells in the presence of C1q. However, enhancement of phagocytosis was observed in all APCs when C1q was added in the presence of serum with active complement. These differences in assay design may explain the lack of enhancement in internalization in our hands, as we only added C1q to serum free conditions to discriminate from the involvement of other serum factors. It is also important to note the differences in the apoptotic target cells used between other studies and ours, as our epithelial cell targets are larger than the conventionally used T cell (Jurkat) targets, and therefore may be more difficult to internalize.
Epithelial cell apoptosis occurs frequently in normal physiology, where the cells are replaced from progenitor cells located at the base of the crypts. [
41,
53,
54]. Apoptotic epithelial cells are shed via extrusion, however at least some of these cells are cleared by phagocytes and neighbouring epithelial cells [
70‐
72]. In this manner, the internalization of epithelial cells provides another means by which the host can sample the environment for antigens associated with damage or infection [
73]. It has also been reported that apoptotic epithelial cells can influence inflammation [
72,
74]. Juncadella et al. demonstrated that apoptotic bronchial epithelial cells can be engulfed by other epithelial cells and that these interactions are important in the control of murine airway inflammation [
74]. Relevant to
H. pylori, a recent study has shown that infection by
H. pylori inhibits the phagocytosis of apoptotic gastric epithelial cells [
75]. Inhibition of clearance and the concomitant increase in the release of necrotic cell contents would trigger a heightened inflammatory response, which would likely inhibit the anti-inflammatory effect conferred by apoptotic cells. Furthermore, decreased phagocytosis would result in the reduced sampling of infected cells, which could lead to reduced antigen presentation and less pathogen specific immune cell attack. These mechanisms may provide an explanation of how a predominantly luminal infection triggers such a potent immune reaction. Future studies should focus on using the more relevant tissue specific macrophages as it is becoming apparent that these cells can respond very differently to microbial insult [
76].
Competing interests
The Authors declare no conflicts of interest.
Authors’ contributions
SF (cytokine studies, apoptosis analysis) KAR, AB, KP and SD designed, performed and analyzed the experiments. SF and KAR wrote the manuscript, SEC consulted on experimental design, PE directed the study. All authors read and approved the final manuscript.