Background
Sepsis occurs when an infection does not remain limited to local tissue but instead induces a series of dysregulated host responses that result in life-threatening organ dysfunction [
1,
2]. Sepsis is one of the leading causes of death in intensive care units (ICUs), and sepsis and septic shock are important healthcare problems. Walkey et al. conducted a nationwide retrospective cohort study that identified 53.9 million adult infection hospitalizations from 2003 to 2009 and found that in the USA, the sepsis incidence rate has increased to 535 cases per 100,000 person-years and continues to rise [
3]. Based on case fatality rates for prior decades, sepsis may cause or contribute to up to 5.3 million deaths worldwide each year [
4,
5].
Global prevalence of overweight and obesity in children and adults from 1980 to 2013 were estimated by Ng et al., who found large increases in the prevalence over time [
6]. Overweight and obesity are medical conditions in which excess body fat has accumulated to such an extent that it may negatively influence health. Studies have shown that obesity is associated with the pathogenesis and prognosis of myriad diseases, such as diabetes, hypertension, and cancer [
7,
8]. However, various recent studies have demonstrated mixed results regarding the role of obesity in diseases. Notably, certain clinical studies addressing the effects of obesity on critical illnesses (such as heart failure, acute coronary syndrome and acute respiratory distress syndrome) have revealed an “obesity paradox” in which obesity is not harmful and can even be protective, including for patients who have already become sick [
9‐
15].
The role of obesity in patient outcomes in specific ICU populations, such as septic patients, has been paid much attention; however, extant clinical data on this topic remain controversial. It is uncertain whether obesity could influence the acute risk of death in sepsis. Studies have indicated that obesity is correlated with an increased risk of death [
16], whereas other investigations have reported inverse [
17‐
19] or null [
20‐
25] associations between obesity and risk of death. Two systematic reviews have analysed the effects of obesity in septic patients. However, a number of additional observational studies on the associations between body mass index (BMI) and risk of death among septic patients have not been included in meta-analysis [
18,
21‐
23]. In addition, these two reviews only focused on the outcome of death and did not examine the effects of BMI on length of stay (LOS) in the ICU or the hospital, which is a major cause of high medical expenses. To further understand this problem, we performed a meta-analysis to address the associations between obesity, which was measured using BMI, and outcomes in sepsis.
Discussion
This meta-analysis showed that patients with BMI ≥ 25 kg/m
2 exhibited decreased mortality compared with that of normal-weight patients, which is an example of the “obesity paradox” and can be explained in several ways. First, sepsis is an acute illness involving a highly catabolic state, and excess body fat may serve as a source of fuel and energy during the course of this disease. In particular, research has indicated that higher body weight provides a nutritional reserve that becomes important for survival during an acute life-threatening illness [
14]. Adipocytes store excess calories as triglycerides and release fuel (as fatty acids and glycerol) for use by other organs in times of caloric need [
29]. Second, adipose tissue can also regulate immunity by excreting proteins such as leptin, which is an anti-inflammatory adipokine [
30]. Obese individuals have elevated leptin levels at baseline. These levels are regulated by several factors; for instance, leptin can be increased by acute infection and proinflammatory cytokines. Bornstein et al. found that in acute sepsis cases, mean plasma leptin levels are threefold higher in survivors than in non-survivors; they assumed that leptin may play a role in a severe stress state such as acute sepsis [
31]. In an animal study, researchers used a long-term cecum ligation and puncture model of sepsis with feeding of a high-fat diet to investigate the effects of obesity on immune function and outcome in sepsis. They showed that relative hyperleptinaemia induced by obesity or treatment is protective in sepsis, possibly via stabilizing body temperature, improving cellular immune response, and reducing proinflammatory cytokine response. Leptin appears to play a regulatory role in the immune system in sepsis [
32]. Levels of adiponectin, an anti-inflammatory adipokine, change during the course of sepsis. In sepsis, higher adiponectin levels before sepsis and decreasing adiponectin levels after sepsis are associated with survival. Additionally, compared with patients who survived sepsis, O’Brien et al. found lower adiponectin concentrations before sepsis in the subgroup of patients who died during the course of sepsis [
33]. Finally, obesity may be treated more aggressively than other diseases, in part because it is associated with high risks of cardiovascular disease, insulin resistance, hypertension, and other comorbidities [
34]. Recent studies showed that treatment of severe sepsis differs according to BMI [
35,
36]. Authors have also observed that similar volumes of resuscitation fluids were administered to sepsis patients irrespective of BMI. Thus, obese patients received a lower volume/kg of such fluids than patients with normal BMI [
20]. In sepsis, improved outcomes for obese patients may be associated with a relatively restrictive fluid management strategy [
37‐
39]. Clinically, physicians caring for adult patients likely do not routinely consider weight and height during decision making. The same situation will arise with respect to the administration of vasopressin or antibiotics. The question of whether weight-based dosing (in terms of mcg/kg/min) is superior to non-weight-based dosing (in terms of mcg/min) for these drugs has not been definitively answered.
In subgroup analysis, we also found that overweight patients had lower mortality than normal-weight patients. Researchers in Denmark have demonstrated that for cohorts enrolled from 1976 to 1978 through 2003–2013, the BMI associated with the lowest all-cause mortality has increased over time to 27.0 kg/m
2 [
40]. In other words, people with BMI of 27.0 kg/m
2, which is in the overweight range, may have the lowest risk of death; this result is consistent with our findings. However, significantly decreased mortality was not observed in obese or morbidly obese patients relative to normal-weight reference patients. Relatively few obese and morbidly obese patients (only 1528 and 376 patients, respectively) were included in our analysis; in addition, due to inherent limitations, existing clinical studies may not have detected acquired comparative survival advantages. For instance, obese and morbidly obese patients may have an inherited genetic susceptibility for developing sepsis and therefore exhibit a higher risk of death. However, the overweight population in these studies may be represented by individuals whose overall health is relatively good. Second, an unmeasured confounding variable in our meta such as smoking, which has been identified to be associated with mortality after severe sepsis, may also explain the aforementioned results [
41]. On the other hand, body fat distribution and body composition may differ between overweight patients and obese or morbidly obese patients, resulting in different effects of excess weight on outcomes in sepsis. Researchers have shown that ectopic fat accumulation is strongly associated with higher metabolic disease risk. Interestingly, subcutaneous fat accumulation confers beneficial systemic metabolic effects, whereas visceral fat does not [
42,
43]. Investigators have used the term “metabolically healthy obesity” to refer to the preferential deposition of fat in subcutaneous depots in a manner that maintains insulin sensitivity, lowers infiltration by inflammatory cells and involves adipocytes with relatively favourable adipokine expression profiles [
44].
Our results can be compared with the findings from the two prior meta-analyses of obesity and mortality after sepsis. A systematic review without analysis included seven studies with mixed results indicating that obesity might increase, reduce or not affect mortality [
45]. Another meta-analysis examined the effect of different BMI categories on mortality after adjusting for other influential baseline variables and found that overweight or obese BMI was associated with reduced adjusted mortality in adults admitted to the ICU with sepsis, severe sepsis, or septic shock [
46]. Our study included more valuable studies and performed further analyses. We found that patients with BMI ≥ 25 kg/m
2 exhibited decreased mortality. Additionally, the subgroup analysis demonstrated that overweight was associated with lower mortality. Although we failed to observe a significant association between obese or morbidly obese BMI and decreased mortality, we also did not find obese or morbidly obese BMI to be correlated with worse outcome. Furthermore, we extracted available data, analysed the adjusted effects of overweight BMI on the OR for mortality relative to normal BMI, and showed that overweight BMI reduced adjusted mortality (Additional file
1: Appendix E). What’s more, we looked at hospital and ICU LOS. Despite these findings, more studies reporting the effects of BMI adjusted for other influential baseline variables are necessary due to the small quantity of currently available data, which may have resulted in bias.
Our study has several limitations. We focus on sepsis in our study but could not exclude the possibility that comorbidities such as diabetes mellitus or coronary heart disease may have improved or worsened outcomes [
47]. Moreover, septic patients in hospitals are treated using different interventions, as described above; these differences introduce added complexity to the examined issue. Although the association between BMI and mortality was adjusted for age, gender and several other variables in certain studies [
19,
20], residual confounders could not be considered and may have played a role in determining outcomes. We used BMI because it is relatively easy to determine and is widely used to measure degree of obesity in observational studies. However, BMI is not measured as consistently as expected. BMI data in the eight included studies are derived differently, including from a database, from self-reported information or from in-hospital measurements. Furthermore, inaccuracies can be introduced if weight is checked after fluid resuscitation. A recent study showed that BMI may not be an accurate predictor of risks associated with obesity. Chalkias et al. used sagittal abdominal diameter (SAD) to measure visceral obesity and found that increased SAD may predict future complications and increased risk for death in a BMI-independent manner [
23]. A deeper understanding of obesity will allow us to further examine the impact of obesity on sepsis with better and more reliable standards.