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Erschienen in: Pediatric Surgery International 2/2018

17.10.2017 | Original Article

The role of intermediate filaments in maintaining integrity and function of intestinal epithelial cells after massive bowel resection in a rat

verfasst von: I. Sukhotnik, Y. Ben Shahar, Y. Pollak, T. Dorfman, H. Kreizman Shefer, Z. E. Assi, N. Mor-Vaknin, A. G. Coran

Erschienen in: Pediatric Surgery International | Ausgabe 2/2018

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Abstract

Purpose

Intermediate filaments (IFs) are a part of the cytoskeleton that extend throughout the cytoplasm of all cells and function in the maintenance of cell-shape by bearing tension and serving as structural components of the nuclear lamina. In normal intestine, IFs provide a tissue-specific three-dimensional scaffolding with unique context-dependent organizational features. The purpose of this study was to evaluate the role of IFs during intestinal adaptation in a rat model of short bowel syndrome (SBS).

Materials and methods

Male rats were divided into two groups: Sham rats underwent bowel transection and SBS rats underwent a 75% bowel resection. Parameters of intestinal adaptation, enterocyte proliferation and apoptosis were determined 2 weeks after operation. Illumina’s Digital Gene Expression (DGE) analysis was used to determine the cytoskeleton-related gene expression profiling. IF-related genes and protein expression were determined using real-time PCR, Western blotting and immunohistochemistry.

Results

Massive small bowel resection resulted in a significant increase in enterocyte proliferation and concomitant increase in cell apoptosis. From the total number of 20,000 probes, 16 cytoskeleton-related genes were investigated. Between these genes, only myosin and tubulin levels were upregulated in SBS compared to sham animals. Between IF-related genes, desmin, vimentin and lamin levels were down-regulated and keratin and neurofilament remain unchanged. The levels of TGF-β, vimentin and desmin gene and protein were down-regulated in resected rats (vs sham animals).

Conclusions

Two weeks following massive bowel resection in rats, the accelerated cell turnover was accompanied by a stimulated microfilaments and microtubules, and by inhibited intermediate filaments. Resistance to cell compression rather that maintenance of cell-shape by bearing tension are responsible for contraction, motility and postmitotic cell separation in a late stage of intestinal adaptation.
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Metadaten
Titel
The role of intermediate filaments in maintaining integrity and function of intestinal epithelial cells after massive bowel resection in a rat
verfasst von
I. Sukhotnik
Y. Ben Shahar
Y. Pollak
T. Dorfman
H. Kreizman Shefer
Z. E. Assi
N. Mor-Vaknin
A. G. Coran
Publikationsdatum
17.10.2017
Verlag
Springer Berlin Heidelberg
Erschienen in
Pediatric Surgery International / Ausgabe 2/2018
Print ISSN: 0179-0358
Elektronische ISSN: 1437-9813
DOI
https://doi.org/10.1007/s00383-017-4192-2

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