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01.12.2012 | Original investigation | Ausgabe 1/2012 Open Access

Cardiovascular Diabetology 1/2012

The role of mediastinal adipose tissue 11β-hydroxysteroid d ehydrogenase type 1 and glucocorticoid expression in the development of coronary atherosclerosis in obese patients with ischemic heart disease

Zeitschrift:
Cardiovascular Diabetology > Ausgabe 1/2012
Autoren:
Fatmahan Atalar, Selcuk Gormez, Baris Caynak, Gokce Akan, Gamze Tanriverdi, Sema Bilgic-Gazioglu, Demet Gunay, Cihan Duran, Belhhan Akpinar, Ugur Ozbek, Ahmet Sevim Buyukdevrim, Zeliha Yazici
Wichtige Hinweise

Electronic supplementary material

The online version of this article (doi:10.​1186/​1475-2840-11-115) contains supplementary material, which is available to authorized users.

Competing interests

The authors declare no conflict of interest.

Authors’ contributions

ASB and FA designed the study. BC and BA did the cardiac surgeries. SG and ASB consulted the patients. FA and GA performed the molecular analysis. CD performed and analyzed the CT scans of the study group. DG performed the biochemical analysis. ZY performed the fatty acids analysis. GT and SBG performed the immunohistochemical staining and analysis. FA, SG, UO and ZY analyzed the data. FA drafted the manuscript. All authors contributed to the final version of the manuscript.

Abstract

Background

Visceral fat deposition and its associated atherogenic complications are mediated by glucocorticoids. Cardiac visceral fat comprises mediastinal adipose tissue (MAT) and epicardial adipose tissue (EAT), and MAT is a potential biomarker of risk for obese patients.

Aim

Our objective was to evaluate the role of EAT and MAT 11beta-hydroxysteroid dehydrogenase type 1 (11β-HSD-1) and glucocorticoid receptor (GCR) expression in comparison with subcutaneous adipose tissue (SAT) in the development of coronary atherosclerosis in obese patients with coronary artery disease (CAD), and to assess their correlations with CD68 and fatty acids from these tissues.

Methods and results

Expression of 11β-HSD-1 and GCR was measured by qRT-PCR in EAT, MAT and SAT of thirty-one obese patients undergoing coronary artery bypass grafting due to CAD (obese CAD group) and sixteen obese patients without CAD undergoing heart valve surgery (controls). 11β-HSD-1 and GCR expression in MAT were found to be significantly increased in the obese CAD group compared with controls (p < 0.05). In the obese CAD group, 11β-HSD-1 and GCR mRNA levels were strongly correlated in MAT. Stearidonic acid was significantly increased in EAT and MAT of the obese CAD group and arachidonic acid was significantly expressed in MAT of the obese male CAD group (p < 0.05).

Conclusions

We report for the first time the increased expression of 11β-HSD-1 and GCR in MAT compared with EAT and SAT, and also describe the interrelated effects of stearidonic acid, HOMA-IR, plasma cortisol and GCR mRNA levels, explaining 40.2% of the variance in 11β-HSD-1 mRNA levels in MAT of obese CAD patients. These findings support the hypothesis that MAT contributes locally to the development of coronary atherosclerosis via glucocorticoid action.
Zusatzmaterial
Authors’ original file for figure 1
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Authors’ original file for figure 2
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Authors’ original file for figure 3
12933_2012_557_MOESM3_ESM.pdf
Authors’ original file for figure 4
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Literatur
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