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01.12.2012 | Research | Ausgabe 1/2012 Open Access

World Journal of Surgical Oncology 1/2012

The role of the 5-hydroxytryptamine pathway in reflux-induced esophageal mucosal injury in rats

Zeitschrift:
World Journal of Surgical Oncology > Ausgabe 1/2012
Autoren:
Lingrong Yang, Haifang Cai, Jinfa Tou, Weizhong Gu, Xiaoli Shu, Ting Zhang, Xi Yang, Zheng Shen, Mizu Jiang
Wichtige Hinweise

Electronic supplementary material

The online version of this article (doi:10.​1186/​1477-7819-10-219) contains supplementary material, which is available to authorized users.

Competing interests

No relevant competing financial and other interests exist for Lingrong Yang, Haifang Cai, Ting Zhang, Weizhong Gu, Jinfa Tou, Xiaoli Shu, Xi Yang, Zheng Shen, and Mizu Jiang.

Authors’ contributions

YL contributed to the study supervision, animal study, data collection, statistical analysis, article drafting, editing, critical revision, and final approval. JM contributed to the conception and study design, study supervision, data analysis, article drafting, editing, critical revision, and final approval. YL, CH, TJ, GW, SX, ZT, YX, and SZ contributed to the GER animal model establishment, HPLC-ECD, RT-PCR, Western blotting, the esophageal histological assessment, data analysis, article editing, and final approval. All authors read and approved the final manuscript.

Abstract

Background

Dysfunction of the 5-hydroxytryptamine (5-HT) signaling pathway can lead to gastrointestinal motility and secretion abnormalities and to visceral hypersensitivity. The aim of this study is to investigate the role of 5-HT in reflux-induced esophageal mucosal injury.

Methods

Fifty 8-week-old male Sprague-Dawley (SD) rats were randomly divided into a gastroesophageal reflux (GER) model group (30 rats) and a sham surgery control group (20 rats). Four weeks after surgery, the esophageal mucosa was collected for histological evaluation, 5-HT concentrations, and 5-HT selective reuptake transporter (SERT) mRNA and 5-HT4 receptor (5-HT4R) protein expressions.

Results

Twenty-seven rats in the GER model group survived, and three rats died. Histologically, in the GER model group, 20 rats had reflux esophagitis (RE group), and 7 rats had non-erosive reflux disease (NERD group). The 5-HT levels in the esophageal tissue from the RE group were significantly higher than those from the control and NERD groups. Both the RE and NERD groups showed significant increases in SERT mRNA expression of the esophageal mucosa than that of the controls, and the SERT mRNA level in the RE group was significantly higher than that in the NERD group. The 5-HT4R protein level of the esophageal mucosa in the RE group was significantly lower than that in the controls and the NERD group.

Conclusions

We conclude that a 5-HT signaling pathway disorder could be a major factor in the pathogenesis of GER and RE.
Zusatzmaterial
Authors’ original file for figure 1
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Authors’ original file for figure 2
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Authors’ original file for figure 3
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Authors’ original file for figure 5
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Authors’ original file for figure 6
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Authors’ original file for figure 7
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Authors’ original file for figure 8
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Authors’ original file for figure 9
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Literatur
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