Background
Pancreatic Cancer (PC) is the fourth leading cause of death due to cancer worldwide [
1]. The incidence and number of deaths caused by PC have been gradually increasing, even as the incidence and mortality of other common cancers have been declining. Surgical resection remains the only chance for cure, but approximately 80–85 % of patients present with advanced un-resectable state at the time of diagnosis. Unfortunately, PC responds poorly to most chemotherapeutic agents; thus, the 5 year survival rate is only approximately 4 % [
2]. Hence, the biological mechanisms that contribute to the development and progression of PC need to be investigated.
MUC4, a large membrane-anchored glycoprotein, is aberrantly expressed in various types of cancers and inflammatory diseases. Its expression is undetectable in normal pancreatic tissue and chronic pancreatitis, but it is highly expressed in pancreatic intraepithelial neoplasia and PC [
3]. We and other researchers have reported that MUC4 is involved in various biological properties of PC cells, including growth, apoptosis, invasion, tumour angiogenesis and drug resistance [
4‐
13].
Alternative splicing, which is often dysregulated in cancer, can produce various isoforms of genes with differential properties and therefore diverse effects on cancer progression [
14,
15]. For the MUC4 gene, 24 distinct splice transcripts have been isolated from various tissue samples as well as cell lines, named from sv0 (the full-length MUC4) to sv21-MUC4, MUC4/X, and MUC4/Y [
16]. Splice variants of MUC4 are present in pancreatic intraepithelial neoplasia and PC but not in the normal pancreas or in chronic pancreatitis [
17‐
21]. Thus, exploring the function of MUC4 splice variants at the protein level may help us to determine their potential functions in pancreatic carcinomas.
MUC4/Y, a MUC4 splice variant, lacks the fragment translated from exon 2 (encodes the tandem repeat domain), including the NIDO, AMOP, vWD, and EGF-like domains, the trans-membrane domain (TM), and the cytoplasmic tail [
15,
16]. MUC4/Y is named by analogy with MUC1/Y. Compared with MUC1, MUC1/Y lacks the randomly repeated amino acids. As a well-established transcript form of MUC1, MUC1/Y has important functions in tumour initiation and progression [
22‐
24]. We have reported that MUC4/Y could stimulate PC cell line proliferation, invasion and suppress apoptosis [
25,
26]. The role of these domains of MUC4/Y is of interest.
The AMOP domain is a novel extracellular domain that is found in some splice variants of MUC4. It is uncommon in the genome, and it has been described in only 4 proteins so far: MUC4, SUSD2, ISM1, and ISM2. The exact features of AMOP domain remain unknown, although it has been suggested functional in cell adhesion [
27‐
29]. In this study, we investigated whether the deletion of the AMOP domain could alter MUC4(MUC4/Y)-mediated tumour biological processes in PC cells. We found that the deletion of AMOP domain could reverse the tumour angiogenesis and metastasis induced by MUC4/Y. The underlying mechanism was the activation of NOTCH3 downstream genes (VEGF-A, MMP-9 and ANG-2) by AMOP domain. This mechanism could be a potential therapeutic target of PC.
Methods
Cell culture
The human pancreatic cancer cell lines PANC-1 and MiaPaCa-2 were purchased from the Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences. They were maintained in DMEM containing 10 % foetal bovine serum (FBS) (Wisent, Canada) and 1 % penicillin/streptomycin (HyClone, Thermo, USA). Human umbilical venous endothelial cells (HUVECs) (ATCC, USA) were cultured in Endothelial Cell Growth Medium. All cell lines were grown in a humidified chamber supplemented with 5 % CO2 at 37 °C.
Lentiviral production and Infection
The MUC4/Y (NM_004532.4, 167736352) and MUC4/Y-AMOPΔ were synthesised artificially and cloned into the pUC57 plasmid (Genscript Co., China). Lentiviral production was achieved using the pUC57 plasmid carrying MUC4/Y (Shanghai SBO Medical Biotechnology Co., China), with a three-plasmid system of pCDH-CMV-MCS-EF1-Puro, pCD/NL-BH*DDD and pLTR-G. The pancreatic cancer cell lines PANC-1 and MiaPaCa-2 were infected following the manufacturer’s instructions. These cell lines were selected by 2 μg/ml bulk puromycin-resistant culturing (Sigma, USA) for one week. Then, MUC4/Y and MUC4/Y-AMOPΔ expression levels were analysed by real time qPCR and western blotting assays. The cells were then subjected to additional assessments as follows.
mRNA extraction and real-time qPCR
Total RNA was extracted from cell lines using TRIzol reagent (Life Technologies, USA), following the manufacturer’s protocols. After spectrophotometric quantification, 1 μg of total RNA was used for reverse transcription (RT) in a final volume of 20 μl with the Prime-Script RT Reagent (Takara, Japan), according to the manufacturer’s instructions. The amounts of cDNA used for the amplification of the target genes were normalised to the human GAPDH gene. Real-time qPCR was performed on a Step One Plus Real-Time PCR System (Life Technologies, USA) using Fast Start Universal SYBR Green Master Mix (Roche, USA). The primers were as follows: MUC4/Y, forward: 5′-GTCCCAGGAATGACAACAC-3′, reverse: 5′-AATGGTGGAAATGATGGTCTG-3′; GAPDH, forward:5′-ATCTCTGCCCCCTCTGCTGA-3′, reverse: 5′-GATGACCTTGCCCACAGCCT-3′; NOTCH1, forward: 5′-GAGGCGTGGCAGACTATGC-3′, reverse: 5′-CTTGTACTCCGTCAGCGTGA-3′; NOTCH2, forward: 5′-CAACCGCAATGGAGGCTATG-3”; reverse: 5′-GCGAAGGCACAATCATCAATGTT-3′; NOTCH3, forward: 5′-TGGCGACCTCACTTACGACT-3′, reverse: 5′-CACTGGCAGTTATAGGTGTTGAC-3′; NOTCH4, forward: 5′-GATGGGCTGGACACCTACAC-3′, reverse: 5′-CACACGCAGTGAAAGCTACCA-3′; ANG-2, forward: 5′-CTGGGCGTTTTGTTGTTGGTC-3′, reverse: 5′-GGTTTGGCATCATAGTGCTGG-3′. Hes-1, forward: 5′-TGGATGCTCTGAAGAAAGATAGC-3′; reverse: 5′-CTCGGTACTTCCCCAGCAC-3′. The 2-ΔΔCT method was used to calculate relative expression levels. Each real-time PCR was performed in triplicates and independently repeated for three times.
Western blotting
Protein from the cell extracts was resolved by electrophoresis and transferred to poly vinylidene difluoride membrane (PVDF). After blocking with 5 % non-fat milk in Tris-buffered saline, membranes were incubated with specific antibodies at 4 °C overnight. The membrane was then incubated with horseradish peroxidase labeled secondary antibodies. Proteins were visualized with the Super Signal West Femto Maximum sensitivity substrate kit (Thermo Scientific, Logan, UT). Western blots were quantified using the software Image Pro Plus version 6. The antibodies to ANG-2 (SC-7015) and GAPDH (SC-365062) were purchased from Santa Cruz Biotechnology (USA). The antibodies to MUC4/Y (MUC4) (#ab60720), VEGF-A (#ab51745), CD31 (#ab28364) and Hes-1 (#ab71559) were bought from Abcam (USA). The selected monoclonal antibody (#ab60720, Abcam, UK) is specifically directed against amino acids 79–189 of human MUC4, which are included in the protein expressed by the MUC4/Y target gene, including the AMOP, NIDO, VWD, and the EGF-like domains. The antibodies to NOTCH3 (#5276), and MMP-9 (#13667) were from Cell Signalling Technology (USA). Pre-stained markers (Thermo Scientific, USA) were used as internal molecular weight standards. Each blot was independently repeated three times.
PANC-1 cells were cultured as described above. When the cells reached 80 % confluence, the culture medium was changed to DMEM without FBS. Following an additional 24 h of culturing, the supernatant was collected as conditioned medium and stored at −80 °C. After thawing at 4 °C overnight, the 50 μl Matrigel (BD, USA) was coated in a 96-well plate and then incubated at 37 °C for at least 1 h to gel. HUVECs were suspended at a density of 2 × 105 cells/ml in the different supernatants. The cell suspensions (100 μl) were added to each Matrigel-coated well. DMEM was used as the negative control. After 5–12 h, tube images were taken using a digital camera attached to an inverted phase-contrast microscope. The total tube length in each well was measured and calculated using Image-Pro Plus (IPP) software.
HUVEC proliferation assay
HUVECs were suspended at a density of 2 × 104 cells/ml, and 100 μl cell suspensions were seeded into 96-well plates. After 24 h, the medium was changed to conditioned medium, as described above. Cell proliferation was assessed using the Cell Counting Kit-8 (CCK8) (Dojindo Laboratories, Japan), following the manufacturer’s protocol. The cells were stained at the indicated time point with 10 μl CCK8 for 4 h at 37 °C in a CO2incubator. The absorbance at 450 nm (OD value) was measured using a micro-plate reader, and the absorbance at 630 nm was used as a reference. The average OD values were used to represent the total cell numbers of each group. All tests were performed in triplicate.
HUVEC migration and invasion assays
Cell migration and invasion assays were performed using a chamber 6.5 mm in diameter with an 8 μm pore size (Corning, USA). The upper chambers were seeded with 1 × 104 HUVEC cells. Subsequently, the different conditioned media were added to the lower chamber. For the invasion assay, the top chamber was coated with 100 μl of 1 mg/ml Matrigel (BD, USA). The cells were incubated at 37 °C for 36 h. After incubation, the cells that did not migrate through the pores and remained in the upper chamber were removed by scraping the membrane with a cotton swab. The migrated cells on the lower side of the membrane were stained with 0.1 % crystal violet (Sigma, USA) for 20 min at 37 °C, followed by washing with PBS and photographing in 10 random fields of view at 10× magnification. The cell numbers were counted and expressed as the average number of cells/field of view. Three independent experiments were performed in each case.
PANC-1 migration and invasion assays
Cell migration and invasion assays were performed using a chamber 6.5 mm in diameter with an 8 μm pore size (Corning, USA). For migration assays, 5 × 104 PANC-EV, PANC-MUC4/Y and PANC-MUC4/Y-AMOPΔ cells were suspended separately in serum-free DMEM plated in the top chamber of inserts. Then, 0.5 ml of 10 % serum-containing DMEM was added to the lower chamber of the well and the cells were allowed to migrate under chemotactic drive at 37 °C for 24 h; the cells in the upper chamber were then removed using cotton swabs. For invasion assays, cells (5 × 104) were seeded on Matrigel-coated membrane inserts. Cells migrating or invading into the bottom of the membrane were stained with 0.1 % crystal violet for 20 min at 37 °C, followed by washing with PBS. Ten random fields from each membrane were photographed and counted for statistical analysis.
Animals
Four-week-old male nude mice (BALB/c-nu (nu/nu)) were purchased from the Shanghai Experiment Animal Center (Chinese Academy of Sciences, China) and housed in specific pathogen-free conditions. This study was conducted in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the Ministry of Health, China. The Ethics Committee of the First Affiliated Hospital of Nanjing Medical University (Permit Number: 2012-SRFA-093) approved the protocol.
Matrigel plug assay
We conducted a Matrigel plug assay to investigate the tumour angiogenesis properties of MUC4/Y and domain deletion. Twenty-four male mice were randomly divided into three groups. PANC-EV, PANC-MUC4/Y, and PANC-MUC4/Y-AMOPΔ cells were re-suspended at 2 × 107 cells/ml in serum-free medium. Aliquots of cells (0.4 ml, 8 × 106 cells) were mixed with 0.4 ml Matrigel and unilaterally injected into the flank of each mouse (100 μl mixture/per flank). Matrigel mixed with medium served as a negative control. The Matrigel plugs were removed 15 days after implantation. Half of the plugs were used for the measurement of haemoglobin content using Drabkin’s reagent (Sigma, USA). The remaining part of the plugs were fixed in 4 % formalin, embedded in paraffin and used for IHC analysis.
To investigate the functional consequences of the MUC4/Y and MUC4/Y-AMOP domain on the metastatic properties of PC cells, orthotopic implantation was carried out. Twenty-four mice were randomly divided into three groups (PANC-EV, PANC-MUC4/Y, PANC-MUC4/Y-AMOPΔ). The PANC-1 derived cells were harvested from sub-confluent cultures and re-suspended in PBS at a concentration of 2 × 106 cells/100 μl. Single-cell suspensions of >95 % viability were used for the assays. The animals were anesthetised with intra-peritoneal 0.1 % pentobarbital sodium. All surgical procedures were performed under sterile conditions. Two million cells suspended in 100 μl of PBS were injected into the pancreas by a 30-gauge needle. The animals were monitored every two days. To determine tumour metastasis, mice were euthanised by CO2 asphyxiation and autopsied 45 days after the implantation of the tumour cells. Regional and distant lymph nodes, liver, lung and other organs suspected of harbouring metastases were routinely formalin-fixed, embedded, sectioned, and stained with hematoxylin and eosin using standard techniques for microscopic examination.
Immunohistochemistry (IHC)
All specimens were fixed in 4 % formalin and embedded in paraffin before IHC analysis. All procedures with reference to our previous reports [
15]. The tumour slides were examined in a blinded manner. Five fields were selected for examination, and the percentage of positive tumour cells and cell-staining intensity were determined.
Micro-vessel density (MVD) was counted by CD31 staining. Five areas with the highest MVD were chosen for counting under 200× magnification. The average number of vessels in the five areas was considered as the MVD level of the case. Any brown-staining endothelial cells or endothelial cell clusters that were clearly separate from adjacent micro-vessels, tumour cells, and other connective tissue elements, were recorded as a single countable micro-vessel. Even those distinct clusters of the stained endothelial cells that might come from the same vessel snaking its way in and out of the section were considered as separate micro-vessels.
VEGF-A assay
Because VEGFA and MMP9 are secreted factors, they mainly work through paracrine. So we used the commercial kits to test the activity of VEGFA and MMP9 in the conditioned medium of different groups. The PANC-EV, PANC-MUC4/Y or PANC-MUC4/Y-AMOPΔ cells were seeded in six-well plates (1.5 × 105 per well), and incubated at 37 °C. After 24 h, the cell culture supernatant was harvested and cell counts were performed after trypsinisation. After collection, the medium was spun at 800 × g for 3 min at 4 °C to remove cell debris. The supernatant was either frozen at −80 °C for later activity assays or assayed immediately using commercially available ELISA kits (R&D systems, USA).
MMP-9 activity assay
The PANC-EV, PANC-MUC4/Y or PANC-MUC4/Y-AMOPΔ cells were seeded in six-well plates and incubated at 37 °C. After 24 h, the medium was removed and the cells were washed with serum-free medium. The cells were then incubated in serum-free medium for 24 h. MMP-9 activity in the medium was detected using the Fluorokine E Human MMP-9 Activity Assay kit (R&D systems, USA), according to the manufacturer’s protocol.
Statistical analysis
Statistical analysis was performed using the SPSS (Statistical Package for the Social Sciences) software package (Version 18.0). Quantitative data are presented as the mean ± SD. Differences in the mean values of two samples were analysed by Student’s t-test. The Kaplan-Meier method was used to determine the survival distributions and overall survival rates, and the significance of differences between survival rates was calculated by the log-rank test. P < 0.05 was considered significant.
Discussion
The MUC4 protein is a large transmembrane type I glycoprotein that contains several important functional domains. However, because of the technical restrictions in cloning and modifying the large cDNA of MUC4, direct experimental proof for the role of individual MUC4 extracellular domain in cancer development and progression has been difficult to obtain.
MUC4/Y, a transcript variant of MUC4, lacks the transcription from exon 2 (the tandem repeat domains) compared with the full length MUC4 [
16]. Thus, the validated functions of this transcript variant may help us to understand the potential functions of MUC4 in PC development and progression. Because MUC4/Y lacks the large fragment of exon 2, we can study the functions of MUC4/Y and the domains it contains (NIDO, AMOP, vWD, EGF-like) using the overexpression lentivirus system.
Some previous studies showed that MUC4 and HER2 interact physically and transduce intracellular signals to promote a series of biological processes in PC cells [
34,
35]. In this study, we conducted the western blot assay again to check the expression of total-ErbB2/phosphor-ErbB2. We found that the expression of total-ErbB2 and phosphor-ErbB2 were increased in PANC-MUC4/Y group, while no obvious change of them was found when the AMOP domain was deleted. So, we concluded that MUC4/Y might activate the ErbB2, while not through the AMOP domain (Additional file
1: Figure S3F).
Other laboratories reported that the MUC4-NIDO domain could contribute to the MUC4-mediated metastasis of PC cells by expressing the engineered MUC4 (mini MUC4) and MUC4 without the NIDO-domains. In these studies, they showed that MUC4-NIDO domain-mediated metastasis might be partly due to its interaction with the endogenous fibulin-2 protein [
36]. Komatsu et al. showed that overexpression of Muc4 (rat Muc4) or MUC4 hindered integrin-mediated cell-cell and cell-ECM adhesion in vitro because of the large size of MUC4 [
37,
38]. In order to avoid changing the spatial structure, we focused on MUC4/Y, a natural transcript variant of MUC4, lacks fragment from exon 2 (~120 KD), from another point of view: signal transduction, in the current study. The results of real-time qPCR and western blotting experiments showed that MUC4/Y could activate NOTCH3 signalling, and the loss of the MUC4/Y-AMOP domain decreased the activity. The NOTCH signalling is abnormally activated in many human malignancies, including pancreatic cancer [
39‐
43]. It is known to play critical roles in the processes of tumour cell angiogenesis, proliferation, invasion, and metastasis [
32,
40,
44‐
47]. Therefore, MUC4/Y-AMOP domain-promoted tumour angiogenesis and metastasis may be partly due to the activation of NOTCH3.
Many studies have documented that VEGF is a critical mediator of angiogenesis and metastasis [
48‐
52]. Here, we showed that MUC4/Y-overexpression increased VEGF-A protein expression. We also found a marked increase in the secreted form of VEGF-A in the conditioned medium of MUC4/Y-overexpressed cells. However, the loss of the AMOP domain reduced the expression of VEGF-A induced by MUC4/Y.
Another important class of molecules involved in tumour metastasis and angiogenesis are the MMPs. It is known that MMPs are critically involved in the processes of tumour cell invasion and metastasis and that MMP-9 is directly associated with angiogenesis and metastatic processes [
4,
32,
46,
53,
54]. In this study, we showed that overexpression of MUC4/Y increased MMP-9 protein levels and activity.
Apart from VEGF and MMPs, ANG-2 plays crucial role in tumour angiogenesis [
55‐
58]. Although the role and mechanism of ANG-2 in tumour angiogenesis has not been fully clarified, experimental studies have demonstrated a close relationship of VEGF and ANG-2 functions in angiogenesis. ANG-2 promotes vessel sprouting in the presence of abundant VEGF, whereas ANG-2 contributed to vessel regression in the absence of VEGF [
59,
60]. There are no reports of a connection between ANG-2 and MUC4/Y (MUC4) yet. However, it has been reported that ANG-2 is involved in NOTCH signalling. In this study, we found that MUC4/Y could increase both mRNA and protein levels of ANG-2, whereas no change was found in the MUC4/Y-AMOP
Δ group.
Finally, we found that DAPT could counteract the increased expression or activities of Hes-1, VEGF-A, MMP-9, and ANG-2 caused by MUC4/Y overexpression. This result demonstrated that NOTCH3 signalling was the key mechanism underlying these complex phenomena.
Based on our results, we speculate one possible mechanism that the MUC4/Y-AMOP domain induces invasion and angiogenesis by activating NOTCH3 to up-regulate the downstream functional genes, such as VEGF-A, MMP-9, and ANG-2. In addition, taking the membrane location into account, we presume that the MUC4/Y-AMOP domain may be the key region involved in MUC4/Y binding to NOTCH3. This mechanism may be important in various biological processes mediated by MUC4. In subsequent experiments, we will attempt to validate our ideas and further ascertain the precise molecular interaction between the MUC4/Y-AMOP domain and NOTCH3. When the functions of MUC4 (MUC4/Y) and AMOP domain in pancreatic cancer are clarified, we could design some specific sequence such as aptamer/siRNA to silence them, in order to reduce, or even block the malignant biological behavior of pancreatic cancer.
Furthermore, we are the first to use a transcript variant model to study the specific domains of a high molecular weight membrane protein that is difficult to overexpress. In the future, the method we used to investigate the AMOP domain can be a model for studying other high molecular weight membrane proteins. The data in this study are also consistent with the hypothesis that MUC4 is a multifunctional target for the treatment of PC.
Acknowledgements
This work was partially supported by the National Natural Science Foundation of China (81272712, 30901421), the National Natural Science Foundation Project of International Cooperation (NSFC-NIH, 812111519), the Program for Development of Innovative Research Team in the First Affiliated Hospital of NJMU, the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD, JX10231801), and the translational research of early diagnosis and comprehensive treatment in pancreatic cancer (The research Special Fund For public welfare industry of health,201202007).