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27.09.2023 | Original Article

Topical application of a TRPA1 antagonist reduced nociception and inflammation in a model of traumatic muscle injury in rats

verfasst von: Sabrina Qader Kudsi, Caren Tatiane de David Antoniazzi, Camila Camponogara, Graziela Moro Meira, Marcella de Amorim Ferreira, Ana Merian da Silva, Diéssica Padilha Dalenogare, Rubya Zaccaron, Carolina dos Santos Stein, Paulo Cesar Lock Silveira, Rafael Noal Moresco, Sara Marchesan Oliveira, Juliano Ferreira, Gabriela Trevisan

Erschienen in: Inflammopharmacology

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Abstract

Musculoskeletal pain is a widely experienced public healthcare issue, especially after traumatic muscle injury. Besides, it is a common cause of disability, but this pain remains poorly managed. However, the pathophysiology of traumatic muscle injury-associated pain and inflammation has not been fully elucidated. In this regard, the transient receptor potential ankyrin 1 (TRPA1) has been studied in inflammatory and painful conditions. Thus, this study aimed to evaluate the antinociceptive and anti-inflammatory effect of the topical application of a TRPA1 antagonist in a model of traumatic muscle injury in rats. The mechanical trauma model was developed by a single blunt trauma impact on the right gastrocnemius muscle of Wistar male rats (250–350 g). The animals were divided into four groups (Sham/Vehicle; Sham/HC-030031 0.05%; Injury/Vehicle, and Injury/HC-030031 0.05%) and topically treated with a Lanette® N cream base containing a TRPA1 antagonist (HC-030031, 0.05%; 200 mg/muscle) or vehicle (Lanette® N cream base; 200 mg/muscle), which was applied at 2, 6, 12, 24, and 46 h after muscle injury. Furthermore, we evaluated the contribution of the TRPA1 channel on nociceptive, inflammatory, and oxidative parameters. The topical application of TRPA1 antagonist reduced biomarkers of muscle injury (lactate/glucose ratio), spontaneous nociception (rat grimace scale), inflammatory (inflammatory cell infiltration, cytokine levels, myeloperoxidase, and N-acetyl-β-d-glucosaminidase activities) and oxidative (nitrite levels and dichlorofluorescein fluorescence) parameters, and mRNA Trpa1 levels in the muscle tissue. Thus, these results demonstrate that TRPA1 may be a promising anti-inflammatory and antinociceptive target in treating muscle pain after traumatic muscle injury.

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Titel: Topical application of a TRPA1 antagonist reduced nociception and inflammation in a model of traumatic muscle injury in rats
verfasst von: Sabrina Qader Kudsi
Caren Tatiane de David Antoniazzi
Camila Camponogara
Graziela Moro Meira
Marcella de Amorim Ferreira
Ana Merian da Silva
Diéssica Padilha Dalenogare
Rubya Zaccaron
Carolina dos Santos Stein
Paulo Cesar Lock Silveira
Rafael Noal Moresco
Sara Marchesan Oliveira
Juliano Ferreira
Gabriela Trevisan
Publikationsdatum: 27.09.2023
Verlag: Springer International Publishing
Erschienen in: Inflammopharmacology
Print ISSN: 0925-4692
Elektronische ISSN: 1568-5608
DOI: https://doi.org/10.1007/s10787-023-01337-3

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Topical application of a TRPA1 antagonist reduced nociception and inflammation in a model of traumatic muscle injury in rats

Abstract

Live-Webinar "Schulterschmerz in der Praxis gezielt managen"

Springer Medizin

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