The online version of this article (doi:10.1186/1752-1947-6-22) contains supplementary material, which is available to authorized users.
The authors declare that they have no competing interests.
MP, KE and IJN designed the project; FMM and AN performed the research; MP analyzed the data; JPB provided critical assessment and advice on experimental protocols; MP and IJN wrote the paper. All authors read and approved the final manuscript.
Providing information on cancerous tissue samples during a surgical operation can help surgeons delineate the limits of a tumoral invasion more reliably. Here, we describe the use of metabolic profiling of a colon biopsy specimen by high resolution magic angle spinning nuclear magnetic resonance spectroscopy to evaluate tumoral invasion during a simulated surgical operation.
Biopsy specimens (n = 9) originating from the excised right colon of a 66-year-old Caucasian women with an adenocarcinoma were automatically analyzed using a previously built statistical model.
Metabolic profiling results were in full agreement with those of a histopathological analysis. The time-response of the technique is sufficiently fast for it to be used effectively during a real operation (17 min/sample). Metabolic profiling has the potential to become a method to rapidly characterize cancerous biopsies in the operation theater.
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- Towards real-time metabolic profiling of a biopsy specimen during a surgical operation by 1H high resolution magic angle spinning nuclear magnetic resonance: a case report
Izzie Jacques Namer
- BioMed Central