Erschienen in:
01.06.2015 | Original Article
Toxicity of Millettia ferruginea darasana (family: Fabaceae) against the larvae and adult ticks of Amblyomma variegatum Fabricius a three-host tick in cattle
verfasst von:
Manash Kumar Choudhury, Yoseph Shiferaw, Ahmed Hussen
Erschienen in:
Journal of Parasitic Diseases
|
Ausgabe 2/2015
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Abstract
The in vitro toxicity of
Millettia ferruginea darasana (family: Fabaceae) was tested against the larvae adult male and female of a three-host tick,
Amblyomma variegatum Fabricius (family: Ixodidae or hard tick), known as ‘tropical bont tick’ parasitic mainly to cattle found in Ethiopia and other equatorial Africa. The 20, 40, 60, 80 and 100 % concentrations of the seed oil extracted with petroleum ether were found to kill all (100 % mortality) larvae after 12, 9, 6, 3 and 1.5 h respectively. The results summarized in the Table
1 was found to be statistically significant at the probability level of
p = 0.05. The 100 % concentration of the oil caused 100 % mortality of adult male, adult female and fully engorged female tick after 5, 7 and 12 h respectively. The root and root bark showed less toxicity. The leaves did not show any toxicity.
Table 1
Toxicity of the seed oil extracted with petroleum ether against the larvae of Amblyomma variegatum at different concentrations and every 1.5 h interval of time
1 | Water (1 ml,0 %) control | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
0 % | 0 % | 0 % | 0 % | 0 % | 0 % | 0 % | 0 % |
2 | 0.2 (20 %) | 0 | 2 ± 0.5 | 5 ± 0.82 | 8 ± 0.82 | 11 ± 0.58 | 12 ± 0.82 | 13 ± 0.96 | 15 ± 1.41 |
0 % | 13 % | 33 % | 53 % | 73 % | 80 % | 87 % | 100 % |
3 | 0.4 (40 %) | 2 ± 0.82 | 3 ± 0.5 | 6 ± 0.96 | 9 ± 0.58 | 12 ± 0.82 | 15 ± 0.96 | | |
13 % | 20 % | 40 % | 60 % | 80 % | 100 % | | |
4 | 0.6 (60 %) | 5 ± 0.96 | 8 ± 0.82 | 11 ± 0.58 | 15 ± 0.5 | | | | |
33 % | 53 % | 73 % | 100 % | | | | |
5 | 0.8 (80 %) | 9 ± 0.82 | 15 ± 1.29 | | | | | | |
60 % | 100 % | | | | | | |
6 | 1.0 (100 %) | 15 ± 0.5 | | | | | | | |
100 % | | | | | | | |