Electronic supplementary material
Cell lines, MM primary cells, and drugs
Virus generation and transduction of MM cells
Cell cycle analysis
Single-cell gel electrophoresis (Comet) assay
Mitochondrial membrane potential and ROS/superoxide analysis
Flow cytometry and degranulation assay
RNA extraction and quantitative real-time-PCR
Gene set enrichment analysis and gene ontology
Immunostaining for confocal microscopy
Tube assay formation
Gene expression datasets analysis
Affymetrix Human Gene 1.0 ST Array (GPL6244)
Centro de Investigación del Cáncer de Salamanca
Analysis of plasma cells from patients with monoclonal gammopathy of undetermined significance (MGUS) (n = 20), smoldering multiple myeloma (sMM) (n = 33), symptomatic MM (n = 41), and healthy donors (n = 5).
Affymetrix Human Exon 1.0ST Array(GPL5175)
Dana-Farber Cancer Institute
Gene expression microarray datasets from CD138 purified plasma cells isolated from 170 patients with newly diagnosed MM and 6 healthy subjects. All patients received triple drug regime—Vincristine, Adriamycin, and Dexamethasone (VAD)—as induction therapy followed by autologous stem cell transplant (ASCT) as a maintenance therapy.
Affymetrix Human Genome U133A Array (GPL96)
Gene expression profile of CD138 purified plasma cells from 22 MGUS, 24 sMM, 69 newly diagnosed MM, 32 relapsed MM, and 15 healthy subjects. Each sample has been further characterized by FISH for the identification of hyperdiploidy.
Affymetrix Human Genome
U133A Array (GPL96)
University of Milan—Fondazione IRCCS Ospedale Maggiore Policlinico
This series of microarray experiments contains the gene expression profiles of immunomagnetically purified CD138+ plasma cells obtained from 5 normal donors, 11 MGUS, 133 MM, and 9 plasma cell leukemia at diagnosis