Background
Methods
Bioinformatics
Cells
Plasmids
Name | Sequences (5′–3′) |
---|---|
CEBP consensus wild-type (CEBPcw) | Sense, tcgagTGCAGATTGCGCAATCTGCAa; anti-sense, agcttTGCAGATTGCGCAATCTGCAc
|
CEBP consensus mutant (CEBPcm) | Sense, tcgagTGCAGAGACTAGTCTCTGCAa; anti-sense, gcttTGCAGAGACTAGTCTCTGCAc |
CEBP consensus wild-type (CEBP1w) | Sense, tcgagGAGGTTGTGAAACCCa; anti-sense, agcttGGGTTTCACAACCTCc
|
CEBP consensus mutant (CEBP1 m) | Sense, tcgagGAGGGAGTAAGCCCCa; anti-sense, agcttGGGTTTCACAACCTCc
|
CEBP2 wild-type (CEBP2w) | Sense, tcgagTGTGTTCTGTAAGCCa; anti-sense, agcttGGCTTACAGAACACAc
|
CEBP2 mutant (CEBP2 m) | Sense, tcgagTGTGGTCTATGCGCCa; anti-sense, agcttGGCGCATAGACCACAc
|
CEBP3 wild-type (CEBP3w) | Sense, tcgagTACGTTTTGTAATTTa; anti-sense, agcttAAATTACAAAACGTAc
|
CEBP3 mutant (CEBP3 m) | Sense, tcgagTACGGTTTATGCTTTa; anti-sense, agcttAAAGCATAAACCGTAc
|
CEBP4 wild-type (CEBP4w) | Sense, tcgagAAGATTCAGAAATCAa; anti-sense, agcttTGATTTCTGAATCTTc
|
CEBP4 mutant (CEBP4 m) | Sense, tcgagAAGAGTCAAAGCTCAa; anti-sense, agcttTGAGCTTTGACTCTTc
|
Small interfering RNA (siRNA)
Quantitative reverse-transcription polymerase chain reaction (qRT-PCR)
Western blotting
Dual-luciferase reporter gene assay
Electrophoretic mobility shift assay (EMSA)
Name | Sequences |
---|---|
CEBP consensus | 5′-TGCAGATTGCGCAATCTGCA-3′ |
CEBP1 (−1726/−1712) | 5′-gaaGAGGTTGTGAAACCCcct-3′ |
CEBP2 (−1684/−1670) | 5′- tccTGTGTTCTGTAAGCCatc-3′ |
CEBP3 (−722/−708) | 5′-tatTACGTTTTGTAATTTtaa-3′ |
CEBP4 (−163/−149) | 5′-tacAAGATTCAGAAATCAccc-3′ |
Chromatin immunoprecipitation (ChIP)
Name | Sequences | Fragment lengths |
---|---|---|
CEBP1/2 | Forward, 5′-ACAAACTTCACAGGTTGAGGGC-3′ reverse, 5′-TAATAAACCAGTGATAATGAGTGTCTTC-3′ | 184 bp |
CEBP3 | Forward, 5′-CAGCCGTCAGCATTATTTGT-3′ reverse, 5′-CGAAGATTATCTTTTAAGATAGGGA-3′ | 220 bp |
CEBP4 | Forward, 5′-GTAAAAGGTGGTGGTAAGAGGGT-3′ reverse, 5′-ACATGCATAATCTATGCTGTGGTT-3′ | 135 bp |