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01.02.2013 | Research Paper

Transcriptional profiling identifies genes induced by hepatocyte-derived extracellular matrix in metastatic human colorectal cancer cell lines

verfasst von: Isabel Zvibel, Adam Wagner, Metsada Pasmanik-Chor, Chen Varol, Varda Oron-Karni, Erwin M. Santo, Zamir Halpern, Revital Kariv

Erschienen in: Clinical & Experimental Metastasis | Ausgabe 2/2013

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Abstract

The milieu of the liver, and in particular hepatocyte-derived extracellular matrix (hECM), is a critical factor regulating development of liver metastases of colorectal cancer (CRC) cells. The present study has investigated genes altered by hECM in CRC cells and particularly by heparan sulfate chains of hepatocyte proteoglycans. Gene profiling analysis shows that after 2 days on hECM, 226 genes are up-regulated more than 2-fold in strongly metastatic SM cells, including genes involved in growth arrest and apoptosis, signal transduction, cell migration, proliferation, communication and angiogenesis, with activation of the erbB signaling network and p53 effectors. Genes down-regulated by hECM include genes involved in lipogenesis and the S phase of the cell cycle. Further studies exploring the kinetics of gene expression after 4 and 7 days culture on hECM show induction of EGF family members and of stem cell markers. In particular, hECM, but not collagen, increases mRNA expression of HB-EGF and colon stem cell marker leucine-rich repeat-containing G protein-coupled receptor 5 (LGR5). Expression of these genes is not induced by hECM depleted of the heparan sulfate chains of proteoglycans. Lastly, a specific cell population positive for cancer stem cell (CSC) markers LGR5, epCAM and CD133, but negative for CD44, appears after 7 days culture on hECM, a population which is reduced by 50 % in cells grown on heparan sulfated-depleted hECM. Collectively, the data suggest that hECM induces growth factors and receptors regulating proliferation of metastatic CRC in the liver and offers a growth advantage for specific populations expressing CSC markers.

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Paget S (1989) Lancet 1889(1):571

Fidler IJ (2003) The pathogenesis of cancer metastasis: the ‘seed and soil’ hypothesis revisited. Nat Rev Cancer 3:453–458PubMedCrossRef

Radinsky R (1993) Paracrine growth regulation of human colon carcinoma organ-specific metastasis. Cancer Metastasis Reviews 12:345–361PubMedCrossRef

Zvibel I, Kraft A (1993) Extracellular matrix and metastasis. In: Zern MA, Reid LM (eds) Extracellular matrix: its chemistry, biology, and pathobiology. first ed. (Eds: ) Marcel Dekker, New York, 559–580

Nguyen DX, Bos PD, Massagué J (2009) Metastasis: from dissemination to organ-specific colonization. Nat Rev Cancer 9:274–284PubMedCrossRef

Steeg PS (2006) Tumor metastasis: mechanistic insights and clinical challenges. Nat Med 12:895–904PubMedCrossRef

Kuo TH, Kubota T, Watanabe M, Furukawa T, Teramoto T, Ishibiki K, Kitajima M, Moossa AR, Penman S, Hoffman RM. Liver colonization competence governs colon cancer metastasis. Proc Natl Acad Sci U S A. 92:12085-9

Doerr R, Zvibel I, Chiuten D, D’Olimpio J, Reid LM (1989) Clonal growth of tumors on tissue-specific biomatrices and correlation with organ-site specificity of metastases. Cancer Res 49:384–392PubMed

Martinez-Hernandez A, Amenta PS. Morphology, localization, and cellular origin of hepatic extracellular matrix. In: Extracellular Matrix: Its Chemistry, Biology and Pathobiology, first ed. (Eds: Zern,M; Reid,LM) Marcel Dekker, New York, NY, 1993, 201-255

10.

Lyon M, Deakin JA, Gallagher JT (1994) Liver heparan sulfate structure. J Biol Chem 269:11208–11215PubMed

11.

Zvibel I, Brill S, Halpern Z, Papa M (1998) Hepatocyte extracellular matrix modulates expression of growth factors and growth factor receptors in human colon cancer cells. Exp Cell Res 245:123–131PubMedCrossRef

12.

Zvibel I, Brill S, Halpern Z, Moskovitz S, Yayon A, Papa M (2001) Soluble and matrix associated heparan sulfate proteoglycans increase expression of erb-B2 and erb-B3 in colon cancer cell lines. Int J Cancer 191:316–321CrossRef

13.

Zvibel I, Halpern Z, Papa M (1998) Extracellular matrix modulates the expression of growth factors and growth factor receptors in liver-colonizing colon cell lines. Int J Cancer 77:295–301PubMedCrossRef

14.

Fishman S, Brill S, Papa MZ, Halpern Z, Zvibel I (2002) Heparin derived-disaccharides modulate proliferation and erb-B2-mediated signal transduction in colon cancer cell lines. Int J Cancer 99:179–184PubMedCrossRef

15.

Morikawa K, Walker SM, Jessup JM, Fidler IJ (1988) In vivo selection of highly metastatic cells from surgical specimens of different primary human colon carcinomas implanted into nude mice. Cancer Res48:194348

16.

Block GD, Locker J, Bowen WC, Petersen BE, Katyal S, Strom SC, Riley T, Howard TA, Michalopoulos GK (1996) Population expansion, clonal growth and specific differentiation patterns in primary cultures of hepatocytes induced by HGF/SF, EGF and TGF α in a chemically defined (HGM) medium. J Cell Biol 132:1133–1149PubMedCrossRef

17.

Berry MN, Friend DJ (1969) High-yield preparation of isolated rat liver parenchymal cells: a biochemical and fine structure study. J Cell Biol 43:506–520PubMedCrossRef

18.

Haggerty JG, Bretton RH, Milstone LM (1994) Response of stratified cultures of human keratinocytes to disruption of proteoglycan synthesis by p-nitrophenyl-beta-d-xylopyranoside. J Cell Physiol 158:39–46PubMedCrossRef

19.

Renné T, Dedio J, David G, Müller-Esterl W (2000) High molecular weight kininogen utilizes heparan sulfate proteoglycans for accumulation on endothelial cells. J Biol Chem 275:33688–33696PubMedCrossRef

20.

Irizarry RA, Hobbs B, Collin F, Beazer-Barclay YD, Antonellis KJ, Scherf U, Speed TP (2003) Exploration, normalization, and summaries of high density oligonucleotide array probe level data. Biostatistics 4:249–264PubMedCrossRef

21.

Chen J, Bardes EE, Aronow BJ, Jegga AG (2009) ToppGene Suite for gene list enrichment analysis and candidate gene prioritization. Nucleic Acids Res 37:W305–W311PubMedCrossRef

22.

Dennis G Jr, Sherman BT, Hosack DA, Yang J, Gao W, Lane HC, Lempicki RA (2003) DAVID: database for annotation, visualization, and integrated discovery. Genome Biol 4:P3PubMedCrossRef

23.

Solinger JA, Paolinelli R, Kloss H, Scorza FB, Marchesi S, Sauder U, Mitsushima D, Capuani F, Stürzenbaum SR, Cassata G (2010) The Caenorhabditis elegans elongator complex regulates neuronal alpha-tubulin acetylation. PLoS Genet 6:e1000820PubMedCrossRef

24.

Mardaryev AN, Meier N, Poterlowicz K, Sharov AA, Sharova TY, Ahmed MI, Rapisarda V, Lewis C, Fessing MY, Ruenger TM, Bhawan J, Werner S, Paus R, Botchkarev VA (2011) Lhx2 differentially regulates Sox9, Tcf4 and Lgr5 in hair follicle stem cells to promote epidermal regeneration after injury. Development 138:4843–4852PubMedCrossRef

25.

Fan XS, Wu HY, Yu HP, Zhou Q, Zhang YF, Huang Q (2010) Expression of Lgr5 in human colorectal carcinogenesis and its potential correlation with beta-catenin. Int J Colorectal Dis 25:583–590PubMedCrossRef

26.

Mueller MT, Hermann PC, Witthauer J, Rubio-Viqueira B, Leicht SF, Huber S, Ellwart JW, Mustafa M, Bartenstein P, D’Haese JG, Schoenberg MH, Berger F, Jauch KW, Hidalgo M, Heeschen C (2009) Combined targeted treatment to eliminate tumorigenic CSCs in human pancreatic cancer. Gastroenterology 137:1102–1113PubMedCrossRef

27.

Ding Q, Chang CJ, Xie X, Xia W, Yang JY, Wang SC, Wang Y, Xia J, Chen L, Cai C, Li H, Yen CJ, Kuo HP, Lee DF, Lang J, Huo L, Cheng X, Chen YJ, Li CW, Jeng LB, Hsu JL, Li LY, Tan A, Curley SA, Ellis LM, Dubois RN, Hung MC (2011) APOBEC3G promotes liver metastasis in an orthotopic mouse model of colorectal cancer and predicts human hepatic metastasis. J. Clin Invest. 121:4526–4536PubMedCrossRef

28.

Onder TT, Gupta PB, Mani SA, Yang J, Lander ES, Weinberg RA (2008) Loss of E-cadherin promotes metastasis via multiple downstream transcriptional pathways. Cancer Res 68:3645–3654PubMedCrossRef

29.

Menendez JA, Lupu R (2007) Fatty acid synthase and the lipogenic phenotype in cancer pathogenesis. Nat Rev Cancer 7:763–777PubMedCrossRef

30.

Radinsky R, Risin S, Fan D, Dong Z, Bielenberg D, Bucana CD, Fidler IJ (1995) Level and function of epidermal growth factor receptor predict the metastatic potential of human colon carcinoma cells. Clin Cancer Res 1:19–31PubMed

31.

Kapitanovic S, Radosevic S, Kapitanovic M, Andelinovic S, Ferencic Z, Tavassoli M, Primorac D, Sonicki Z, Spaventi S, Pavelic K, Spaventi R (1997) The expression of p185 (HER-2/neu) correlates with the stage of disease and survival in colorectal cancer. Gastroenterology 112:1103–1113PubMedCrossRef

32.

Yotsumoto F, Yagi H, Suzuki SO, Oki E, Tsujioka H, Hachisuga T, Sonoda K, Kawarabayashi T, Mekada E, Miyamoto S (2008) Validation of HB-EGF and amphiregulin as targets for human cancer therapy. Biochem Biophys Res Commun 365:555–561PubMedCrossRef

33.

Barkan D, Green JE, Chambers AF (2010) Extracellular matrix: a gatekeeper in the transition from dormancy to metastatic growth. Eur J Cancer 46:1181–1188PubMedCrossRef

34.

Polyak K, Weinberg RA (2009) Transitions between epithelial and mesenchymal states: acquisition of malignant and stem cell traits. Nat Rev Cancer 9:265–273PubMedCrossRef

35.

Hugo H, Ackland ML, Blick T, Lawrence MG, Clements JA, Williams ED, Thompson EW (2007) Epithelial-mesenchymal and mesenchymal-epithelial transitions in carcinoma progression. J Cell Physiol 213:374–383PubMedCrossRef

36.

Chaffer CL, Thompson EW, Williams ED (2007) Mesenchymal to epithelial transition in development and disease. Cells Tissues Organs 185:7–19PubMedCrossRef

37.

Wells A, Yates C, Shepard CR (2008) E-cadherin as an indicator of mesenchymal to epithelial reverting transitions during the metastatic seeding of disseminated carcinomas. Clin Exp Metastasis 25:621–628PubMedCrossRef

38.

Zhou BB, Zhang H, Damelin M, Geles KG, Grindley JC, Dirks PB (2009) Tumour-initiating cells: challenges and opportunities for anticancer drug discovery. Nat Rev Drug Discov. 8:806–823PubMedCrossRef

39.

Dieter SM, Ball CR, Hoffmann CM, Nowrouzi A, Herbst F, Zavidij O, Abel U, Arens A, Weichert W, Brand K, Koch M, Weitz J, Schmidt M, von Kalle C, Glimm H (2011) Distinct types of tumor-initiating cells form human colon cancer tumors and metastases. Cell Stem Cell 9:357–365PubMedCrossRef

40.

Todaro M, Francipane MG, Medema JP, Stassi G (2010) Colon CSCs: promise of targeted therapy. Gastroenterology 138:2151–2162PubMedCrossRef

41.

Walker F, Zhang HH, Odorizzi A, Burgess AW (2011) LGR5 is a negative regulator of tumourigenicity, antagonizes wnt signaling and regulates cell adhesion in colorectal cancer cell lines. PLoS ONE 6:e22733PubMedCrossRef

42.

Chiu ST, Chang KJ, Ting CH, Shen HC, Li H, Hsieh FJ (2009) Over-expression of EphB3 enhances cell–cell contacts and suppresses tumor growth in HT-29 human colon cancer cells. Carcinogenesis 30:1475–1486PubMedCrossRef

43.

Guo DL, Zhang J, Yuen ST, Tsui WY, Chan AS, Ho C, Ji J, Leung SY, Chen X (2006) Reduced expression of EphB2 that parallels invasion and metastasis in colorectal tumours. Carcinogenesis 27:454–464PubMedCrossRef

44.

Barker N, Ridgway RA, van Es JH, van de Wetering M, Begthel H, van den Born M, Danenberg E, Clarke AR, Sansom OJ, Clevers H (2009) Crypt stem cells as the cells-of-origin of intestinal cancer. Nature 457:608–611PubMedCrossRef

45.

Vermeulen L, De Sousa E, Melo F, van der Heijden M, Cameron K, de Jong JH, Borovski T, Tuynman JB, Todaro M, Merz C, Rodermond H, Sprick MR, Kemper K, Richel DJ, Stassi G, Medema JP (2010) Wnt activity defines colon CSCs and is regulated by the microenvironment. Nat Cell Biol 12:468–476PubMedCrossRef

Titel: Transcriptional profiling identifies genes induced by hepatocyte-derived extracellular matrix in metastatic human colorectal cancer cell lines
verfasst von: Isabel Zvibel
Adam Wagner
Metsada Pasmanik-Chor
Chen Varol
Varda Oron-Karni
Erwin M. Santo
Zamir Halpern
Revital Kariv
Publikationsdatum: 01.02.2013
Verlag: Springer Netherlands
Erschienen in: Clinical & Experimental Metastasis / Ausgabe 2/2013
Print ISSN: 0262-0898
Elektronische ISSN: 1573-7276
DOI: https://doi.org/10.1007/s10585-012-9527-8

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Transcriptional profiling identifies genes induced by hepatocyte-derived extracellular matrix in metastatic human colorectal cancer cell lines

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