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01.12.2017 | Research | Ausgabe 1/2017 Open Access

Virology Journal 1/2017

Transcriptome analysis of Cucumis sativus infected by Cucurbit chlorotic yellows virus

Zeitschrift:
Virology Journal > Ausgabe 1/2017
Autoren:
Xinyan Sun, Zhenyue Wang, Qinsheng Gu, Honglian Li, Weili Han, Yan Shi
Wichtige Hinweise

Electronic supplementary material

The online version of this article (doi:10.​1186/​s12985-017-0690-z) contains supplementary material, which is available to authorized users.

Abstract

Background

Cucurbit chlorotic yellows virus (CCYV) is a recently reported bipartite crinivirus that causes chlorotic leaf spots and yellowing symptoms on the leaves of cucurbit plants. The virus–host interaction of CCYV remains to be elucidated, and the influence of criniviruses on the host gene transcriptome requires analysis.

Methods

We used transcriptome sequencing to analyse the differentially expressed genes (DEGs) caused by CCYV infection.

Results

CCYV infection resulted in 865 DEGs. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis identified 67 pathways, and the three major enrichment pathways (according to the P-values) were photosynthesis-antenna proteins (KO00196), phenylalanine metabolism (KO00360a), and phenylpropanoid biosynthesis (KO00940). Of the 13 DEGs identified in phenylalanine metabolism, 11 genes encode disease resistance-related phenylalanine ammonia-lyase (PAL) genes. Using quantitative real-time PCR, we validated the differential expression of 12 genes.

Conclusions

Our study based on the CCYV–cucumber interaction provides comprehensive transcriptomic information, and will improve our understanding of host–crinivirus interactions.
Zusatzmaterial
Additional file 1: Figure S1. Volcano plot of the transcriptome results. Red points represent up-regulation, green points represent down-regulation, and black points represent no difference. (JPG 59 kb)
12985_2017_690_MOESM1_ESM.jpg
Additional file 2: Table S1. List of DEGs classified as cellular component genes. (XLS 33 kb)
12985_2017_690_MOESM2_ESM.xls
Additional file 3: Table S2. List of DEGs classified as molecular function genes. (XLS 4 kb)
12985_2017_690_MOESM3_ESM.xls
Additional file 4: Table S3. List of DEGs classified as biological process genes. (XLS 4 kb)
12985_2017_690_MOESM4_ESM.xls
Additional file 5: Figure S2. Significantly enriched GO terms. (JPG 6565 kb)
12985_2017_690_MOESM5_ESM.jpg
Additional file 6: Figure S3. Pipeline for GO annotation. (JPG 24 kb)
12985_2017_690_MOESM6_ESM.jpg
Additional file 7: Table S4. Ten Significantly enriched pathways. (DOC 37 kb)
12985_2017_690_MOESM7_ESM.doc
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