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01.12.2018 | Research | Ausgabe 1/2018 Open Access

Virology Journal 1/2018

Transcriptome profiling of whitefly guts in response to Tomato yellow leaf curl virus infection

Zeitschrift:
Virology Journal > Ausgabe 1/2018
Autoren:
Liang Geng, Li-Xin Qian, Ruo-Xuan Shao, Yin-Quan Liu, Shu-Sheng Liu, Xiao-Wei Wang
Wichtige Hinweise

Electronic supplementary material

The online version of this article (https://​doi.​org/​10.​1186/​s12985-018-0926-6) contains supplementary material, which is available to authorized users.

Abstract

Background

Plant viruses in agricultural crops are of great concern worldwide, and over 75% of them are transmitted from infected to healthy plants by insect vectors. Tomato yellow leaf curl virus (TYLCV) is a begomovirus, which is the largest and most economically important group of plant viruses, transmitted by the whitefly Bemisia tabaci. The circulation of TYLCV in the insect involves complex insect-virus interactions, whereas the molecular mechanisms of these interactions remain ambiguous. The insect gut as a barrier for viral entry and dissemination is thought to regulate the vector specificity. However, due to its tiny size, information for the responses of whitefly gut to virus infection is limited.

Methods

We investigated the transcriptional response of the gut of B. tabaci Middle East-Asia Minor 1 species to TYLCV infection using Illumina sequencing.

Results

A total of 5207 differentially expressed genes (DEGs) between viruliferous and non-viruliferous whitefly guts were identified. Enrichment analyses showed that cargo receptor and ATP-binding cassette (ABC) transporters were enriched in DEGs, and might help the virus to cross gut barrier. TYLCV could perturb cell cycle and DNA repair as a possible result of its replication in the whitefly. Our data also demonstrated that TYLCV can activate whitefly defense responses, such as antimicrobial peptides. Meanwhile, a number of genes involved in intracellular signaling were activated by TYLCV infection.

Conclusions

Our results reveal the complex insect-virus relationship in whitefly gut and provide substantial molecular information for the role of insect midguts in virus transmission.
Zusatzmaterial
Additional file 1: Table S1. DEGs involved in Lysosome. Displayed are the fold changes of DEGs involved in Lysosome of viruliferous guts in comparison to nonviruliferous guts. aThe function of the homologous gene. bFC, fold change (log2 ratio) of gene expression. (XLSX 8 kb)
12985_2018_926_MOESM1_ESM.xlsx
Additional file 2: Table S2. Sequences of primers used in the study. Displayed are the sequence of the primers used for qRT-PCR. Primers were synthesized by GenScript (Nanjing, China). (XLSX 9 kb)
12985_2018_926_MOESM2_ESM.xlsx
Literatur
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