nach oben

Clinical & Experimental Metastasis

Erschienen in:

28.10.2018 | Technical Note

Transparent reporting of experimental parameters in assays measuring phenotypic steps in metastasis

verfasst von: Robin Boiy, Jonas Steenbrugge, Jan Van Deun, An Hendrix, Evelyne Meyer, Olivier De Wever

Erschienen in: Clinical & Experimental Metastasis | Ausgabe 8/2018

Einloggen, um Zugang zu erhalten

Abstract

Metastasis is key to cancer mortality. Understanding its biology is vital for developing strategies to prevent and treat metastasis. Phenotypic assays to either study metastasis or evaluate anti-metastatic drugs are widely used in preclinical research. This technical note discusses the adherence of reporting essential experimental and methodological parameters in chemotactic invasion assays in vitro and spontaneous metastasis assays in vivo. Following the analysis of 130 recent (< 5 years) research papers, several shortcomings in reporting were identified. Therefore, we strongly argue to increase experimental rigor which should result in a significant improvement with respect to reproducibility of preclinical metastasis research.

Fitzmaurice C, Allen C, Barber RM et al (2017) Global, regional, and national cancer incidence, mortality, years of life lost, years lived with disability, and disability-adjusted life-years for 32 cancer groups, 1990 to 2015: a systematic analysis for the global burden of disease study global burden of disease cancer collaboration. JAMA Oncol 3:524–548. https://doi.org/10.1001/jamaoncol.2016.5688 CrossRefPubMed

Hanahan D, Weinberg RA (2011) Hallmarks of cancer: the next generation. Cell 144:646–674. https://doi.org/10.1016/j.cell.2011.02.013 CrossRefPubMed

Hanahan D, Weinberg RA (2000) The hallmarks of cancer. Cancer Immunol Immunother 100:57–70. https://doi.org/10.1007/s00262-010-0968-0 CrossRef

Richardson MM, Jennings LK, Zhang XA (2011) Tetraspanins and tumor progression. Clin Exp Metastasis 28:261–270. https://doi.org/10.1007/s10585-010-9365-5 CrossRefPubMed

Hu M, Polyak K (2008) Molecular characterisation of the tumour microenvironment in breast cancer. Eur J Cancer 44:2760–2765. https://doi.org/10.1016/j.ejca.2008.09.038 CrossRefPubMedPubMedCentral

Nguyen DX, Bos PD, Massagué J (2009) Metastasis: from dissemination to organ-specific colonization. Nat Rev Cancer 9:274–284. https://doi.org/10.1038/nrc2622 CrossRefPubMed

Kramer N, Walzl A, Unger C et al (2013) In vitro cell migration and invasion assays. Mutat Res Rev Mutat Res 752:10–24. https://doi.org/10.1016/j.mrrev.2012.08.001 CrossRef

Albini A, Benelli R (2007) The chemoinvasion assay: a method to assess tumor and endothelial cell invasion and its modulation. Nat Protoc 2:504–511. https://doi.org/10.1038/nprot.2006.466 CrossRefPubMed

Kleinman HK, Martin GR (2005) Matrigel: basement membrane matrix with biological activity. Semin Cancer Biol 15:378–386. https://doi.org/10.1016/j.semcancer.2005.05.004 CrossRefPubMed

10.

Glentis A, Gurchenkov V, Vignjevic DM (2014) Assembly, heterogeneity, and breaching of the basement membranes. Cell Adhes Migr 8:236–245. https://doi.org/10.4161/cam.28733 CrossRef

11.

Schoumacher M, Glentis A, Gurchenkov VV, Vignjevic DM (2013) Basement membrane invasion assays: native basement membrane and chemoinvasion assay. Methods Mol Biol 1046:133–144. https://doi.org/10.1007/978-1-62703-538-5_8 CrossRefPubMed

12.

Ke N, Wang X, Xu X, Abassi YA (2011) The xCELLigence system for real-time and label-free monitoring of cell viability. Methods Mol Biol 740:33–43. https://doi.org/10.1007/978-1-61779-108-6_6 CrossRefPubMed

13.

Bird C, Kirstein S (2009) Real-time, label-free monitoring of cellular invasion and migration with the xCELLigence system. Nat Methods 6:5–6. https://doi.org/10.1038/nmeth.f.263 CrossRef

14.

O’Clair L, Syzbut C, Appledorn DM (2017) Visualize chemotaxis in real time. Genet Eng Biotechnol News 37:12–13. https://doi.org/10.1089/gen.37.07.08 CrossRef

15.

Limame R, Wouters A, Pauwels B et al (2012) Comparative analysis of dynamic cell viability, migration and invasion assessments by novel real-time technology and classic endpoint assays. PLoS One 7:1–12. https://doi.org/10.1371/journal.pone.0046536 CrossRef

16.

Paoloni M, Khanna C (2008) Translation of new cancer treatments from pet dogs to humans. Nat Rev Cancer 8:147–156. https://doi.org/10.1038/nrc2273 CrossRefPubMed

17.

Direcks WGE, van Gelder M, Lammertsma AA, Molthoff C (2008) A new rat model of human breast cancer for evaluating efficacy of new anti-cancer agents in vivo. Cancer Biol Ther 7:532–537. https://doi.org/10.4161/cbt.7.4.5481 CrossRefPubMed

18.

Jonkers J, Derksen PWB (2007) Modeling metastatic breast cancer in mice. J Mammary Gland Biol Neoplasia 12:191–203. https://doi.org/10.1007/s10911-007-9050-8 CrossRefPubMed

19.

Kocatürk B, Versteeg HH (2015) Orthotopic injection of breast cancer cells into the mammary fat pad of mice to study tumor growth. J Vis Exp. https://doi.org/10.3791/51967 CrossRefPubMedPubMedCentral

20.

Kittrell F, Valdez K, Elsarraj H, Hong Y, Medina D, Behbod F (2016) Mouse mammary intraductal (MIND) method for transplantation of patient derived primary DCIS cells and cell lines. Bio Protoc 6:e1744CrossRefPubMed

21.

Lodillinsky C, Infante E, Guichard A et al (2016) p63/MT1-MMP axis is required for in situ to invasive transition in basal-like breast cancer. Oncogene 35:344–357. https://doi.org/10.1038/onc.2015.87 CrossRefPubMed

22.

Welch DR (1997) Technical considerations for studying cancer metastasis in vivo. Clin Exp Metastasis 15:272–306. https://doi.org/10.1023/A:1018477516367 CrossRefPubMed

23.

Tao K, Fang M, Alroy J, Sahagian GG (2008) Imagable 4T1 model for the study of late stage breast cancer. BMC Cancer 8:228. https://doi.org/10.1186/1471-2407-8-228 CrossRefPubMedPubMedCentral

24.

Steenbrugge J, Breyne K, Denies S et al (2016) Comparison of the adipose and luminal mammary gland compartment as orthotopic inoculation sites in a 4T1-based immunocompetent preclinical model for triple-negative breast cancer. J Mammary Gland Biol Neoplasia 21:113–122. https://doi.org/10.1007/s10911-016-9362-7 CrossRefPubMed

25.

Lou Y, Preobrazhenska O, Auf Dem Keller U et al (2008) Epithelial–mesenchymal transition (EMT) is not sufficient for spontaneous murine breast cancer metastasis. Dev Dyn 237:2755–2768. https://doi.org/10.1002/dvdy.21658 CrossRefPubMed

26.

Lifsted T, Le Voyer T, Williams M, et al (1998) Identification of inbred mouse strains harboring genetic modifiers of mammary tumor age of onset and metastatic progression. Int J Cancer 77:640–644. https://doi.org/10.1002/(SICI)1097-0215(19980812)77:4%3C640::AID-IJC26%3E3.0.CO;2-8 CrossRefPubMed

27.

Borowsky AD, Namba R, Young LJT et al (2005) Syngeneic mouse mammary carcinoma cell lines: two closely related cell lines with divergent metastatic behavior. Clin Exp Metastasis 22:47–59. https://doi.org/10.1007/s10585-005-2908-5 CrossRefPubMed

28.

Bao L, Cardiff RD, Steinbach P et al (2015) Multipotent luminal mammary cancer stem cells model tumor heterogeneity. Breast Cancer Res 17:137. https://doi.org/10.1186/s13058-015-0615-y CrossRefPubMedPubMedCentral

29.

Hurez V, Padrón Á, Svatek RS, Curiel TJ (2018) Considerations for successful cancer immunotherapy in aged hosts. Exp Gerontol 107:27–36. https://doi.org/10.1016/j.exger.2017.10.002 CrossRefPubMed

30.

Jackaman C, Nelson DJ (2014) Are macrophages, myeloid derived suppressor cells and neutrophils mediators of local suppression in healthy and cancerous tissues in aging hosts? Exp Gerontol 54:53–57. https://doi.org/10.1016/j.exger.2013.11.009 CrossRefPubMed

31.

Kaur A, Webster MR, Marchbank K et al (2016) SFRP2 in the aged microenvironment drives melanoma metastasis and therapy resistance. Nature 532:250–254. https://doi.org/10.1038/nature17392 CrossRefPubMedPubMedCentral

32.

Van den Broeck W, Derore A, Simoens P (2006) Anatomy and nomenclature of murine lymph nodes: descriptive study and nomenclatory standardization in BALB/cAnNCrl mice. J Immunol Methods 312:12–19. https://doi.org/10.1016/j.jim.2006.01.022 CrossRefPubMed

33.

Speak AO, Swiatkowska A, Karp NA et al (2017) A high-throughput in vivo screening method in the mouse for identifying regulators of metastatic colonization. Nat Protoc 12:2465–2477. https://doi.org/10.1038/nprot.2017.118 CrossRefPubMed

34.

Sflomos G, Dormoy V, Metsalu T et al (2016) A preclinical model for ERα-positive breast cancer points to the epithelial microenvironment as determinant of luminal phenotype and hormone response. Cancer Cell 29:407–422. https://doi.org/10.1016/j.ccell.2016.02.002 CrossRefPubMed

35.

McDaniel SM, Rumer KK, Biroc SL et al (2006) Remodeling of the mammary microenvironment after lactation promotes breast tumor cell metastasis. Am J Pathol 168:608–620. https://doi.org/10.2353/ajpath.2006.050677 CrossRefPubMedPubMedCentral

36.

Schedin P (2006) Pregnancy-associated breast cancer and metastasis. Nat Rev Cancer 6:281–291. https://doi.org/10.1038/nrc1839 CrossRefPubMed

37.

Feys L, Descamps B, Vanhove C et al (2016) Quantitative and functional requirements for bioluminescent cancer models. In Vivo 30:1–11PubMed

38.

Abt MA, Grek CL, Ghatnekar GS, Yeh ES (2016) Evaluation of lung metastasis in mouse mammary tumor models by quantitative real-time PCR. J Vis Exp. https://doi.org/10.3791/53329 CrossRefPubMedPubMedCentral

39.

Johansen JS, Jensen BV, Roslind A et al (2006) Serum YKL-40, a new prognostic biomarker in cancer patients? Cancer Epidemiol Biomark Prev 15:194–202. https://doi.org/10.1158/1055-9965.EPI-05-0011 CrossRef

40.

Bauer M, Eickhoff JC, Gould MN et al (2008) Neutrophil gelatinase-associated lipocalin (NGAL) is a predictor of poor prognosis in human primary breast cancer. Breast Cancer Res Treat 108:389–397. https://doi.org/10.1007/s10549-007-9619-3 CrossRefPubMed

41.

Domínguez-Vigil IG, Moreno-Martínez AK, Wang JY et al (2018) The dawn of the liquid biopsy in the fight against cancer. Oncotarget 9:2912–2922. https://doi.org/10.18632/oncotarget.23131 CrossRefPubMed

42.

Landis SC, Amara SG, Asadullah K et al (2012) A call for transparent reporting to optimize the predictive value of preclinical research. Nature 490:187–191. https://doi.org/10.1038/nature11556 CrossRefPubMedPubMedCentral

43.

Kilkenny C, Browne WJ, Cuthill IC et al (2013) Improving bioscience research reporting: the arrive guidelines for reporting animal research. Animals 4:35–44. https://doi.org/10.3390/ani4010035 CrossRef

44.

Van Deun J, Mestdagh P, Agostinis P et al (2017) EV-TRACK: transparent reporting and centralizing knowledge in extracellular vesicle research. Nat Methods 14(3):228–232. https://doi.org/10.1038/nmeth.4185 CrossRefPubMed

Titel: Transparent reporting of experimental parameters in assays measuring phenotypic steps in metastasis
verfasst von: Robin Boiy
Jonas Steenbrugge
Jan Van Deun
An Hendrix
Evelyne Meyer
Olivier De Wever
Publikationsdatum: 28.10.2018
Verlag: Springer Netherlands
Erschienen in: Clinical & Experimental Metastasis / Ausgabe 8/2018
Print ISSN: 0262-0898
Elektronische ISSN: 1573-7276
DOI: https://doi.org/10.1007/s10585-018-9944-4

Neu im Fachgebiet Onkologie

19.04.2024 | EAU 2024 | Kongressbericht | Nachrichten

Live-Webinar: Aktuelle Leitlinien bei Herz-Kreislauf-Erkrankungen

Springer Medizin

Transparent reporting of experimental parameters in assays measuring phenotypic steps in metastasis

Abstract

Neu im Fachgebiet Onkologie

Prostatakarzinom: EU initiiert neues Screeningkonzept

Blasenkarzinom – Biomarker statt Zytologie?

Stereotaktische Radiatio schlägt konventionelle Bestrahlung

Adjuvante Brustkrebstherapie: Doppelt hält besser

Update Onkologie

Live-Webinar: Aktuelle Leitlinien bei Herz-Kreislauf-Erkrankungen

Springer Medizin

Abstract

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 8/2018

miR-21 expression analysis in budding colon cancer cells by confocal slide scanning microscopy

Systematic analysis of parameters predicting pathological axillary status (ypN0 vs. ypN+) in patients with breast cancer converting from cN+ to ycN0 through primary systemic therapy (PST)

Role of simvastatin in tumor lymphangiogenesis and lymph node metastasis

Dickkopf-1 (Dkk1) protein expression in breast cancer with special reference to bone metastases

Radiotherapy in palliation of thoracic tumors: a phase I–II study (SHARON project)

Long-chain omega-3 polyunsaturated fatty acids decrease mammary tumor growth, multiorgan metastasis and enhance survival

Neu im Fachgebiet Onkologie

Prostatakarzinom: EU initiiert neues Screeningkonzept

Blasenkarzinom – Biomarker statt Zytologie?

Stereotaktische Radiatio schlägt konventionelle Bestrahlung

Adjuvante Brustkrebstherapie: Doppelt hält besser

Update Onkologie