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01.12.2018 | Research article | Ausgabe 1/2018 Open Access

BMC Musculoskeletal Disorders 1/2018

Triamcinolone Acetonide affects TGF-β signaling regulation of fibrosis in idiopathic carpal tunnel syndrome

Zeitschrift:
BMC Musculoskeletal Disorders > Ausgabe 1/2018
Autoren:
Tai-Hua Yang, Anne Gingery, Andrew R. Thoreson, Dirk R. Larson, Chunfeng Zhao, Peter C. Amadio
Wichtige Hinweise

Electronic supplementary material

The online version of this article (https://​doi.​org/​10.​1186/​s12891-018-2260-y) contains supplementary material, which is available to authorized users.

Abstract

Background

Fibroblast behavior and cell-matrix interactions of cells from normal and idiopathic carpal tunnel syndrome (CTS) subsynovial connective tissue (SSCT) with and without Triamcinolone Acetonide (TA) were compared in this study. A cell-seeded gel contraction model was applied to investigate the effect of steroid treatment on SSCT fibroblast gene expression and function.

Methods

SSCT cells were obtained from CTS patients and fresh cadavers. Cells were isolated by mechanical and collagenase digestion. Collagen gels (1 mg/ml) were prepared with SSCT cells (1 × 106/mL). A sterile Petri dish with a cloning ring in the center was prepared. The area between the ring and outer dish was filled with cell-seeded collagen solution and gelled for 1 h. The gel was released from the outer way of the petri dish to allow gel contraction. Cell seeded gels were treated with 10 M triamcinolone acetonide (TA) or vehicle (DMSO) in modified MEM. Every 4 h for 3 days the contracting gels were photographed and areas calculated. Duplicate contraction tests were performed with each specimen, and the averages were used in the analyses, which were conducted using two-factor analysis of variance in a generalized linear model framework utilizing generalized estimating equations (GEE) to account for the correlation between samples. The contraction rate was determined by the area change over time, and the decay time constant was calculated. A customized mechanical test system was used to determine gel stiffness and tensile strength. Gene expression was assessed using Human Fibrosis and Cell Motility PCR arrays.

Results

TA-treated gels had a significantly higher contraction rate, tensile strength and stiffness than the untreated gels. Proteinases involved in remodeling had increased expression in TA-treated gels of the patient group. Pro-fibrotic genes and ECM regulators, such as TGF-β, collagens and integrins, were down-regulated by TA, indicating that TA may work in part by decreasing fibrotic gene expression.

Conclusions

This study showed that TA affects cell-matrix interaction and suppresses fibrotic gene expression in the SSCT cells of CTS patients.
Zusatzmaterial
Additional file 1: Table S1. Gene fold changes of the CTS group without TA treatment compared to the control group without TA treatment in Human Fibrosis Array (XLS 84 kb)
12891_2018_2260_MOESM1_ESM.xls
Additional file 2: Table S2. Gene fold changes of the CTS group without TA treatment compared to the control group without TA treatment in Cell Motility Array. (XLS 84 kb)
12891_2018_2260_MOESM2_ESM.xls
Additional file 3: Table S3. Gene fold changes of the CTS group with TA treatment compared to the CTS group without TA treatment in Human fibrosis array. (XLS 84 kb)
12891_2018_2260_MOESM3_ESM.xls
Additional file 4: Table S4. Gene fold changes of the CTS group with TA treatment compared to the CTS group without TA treatment in Cell Motility Array. (XLS 84 kb)
12891_2018_2260_MOESM4_ESM.xls
Literatur
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