Triglycerides and total cholesterol concentrations in association with IFG/IGT in Chinese adults in Qingdao, China

Pre-diabetes refers to a condition in elevating plasm glucose, and that is consider normal levels but is not high enough to be classified as diabetes. Pre-diabetes has been defined by impaired fasting glucose (IFG) and/or impaired glucose tolerance (IGT) according to the 2006 World Health Organization (WHO)/International Diabetes Federation (IDF) criteria [1]. Individuals with IFG/IGT were at about 5–10% high risk of developing diabetes worldwide [2, 3]. In China, the prevalence of IFG/IGT is surprisingly high and continues to increase. In a cross-sectional study reported in 2007–2008 involving a nationally representative sample of 46,239 adults, 20 years of age or older, the prevalence of IFG/IGT15.5% [4], and which is far higher than previously reported figures [5]. In additional, with the high epidemiological status of IFG/IGT, they also have a high risk of dyslipidemia [6].

Dyslipidemia can be defined by abnormal level of triglycerides (TG) and total cholesterol (TC). However, the association of TG, TC and IFG/IGT is still not clear. Existing researches demonstrated that hyperglycaemiawas in concert with the potential risk factors of cardiovascular diseases [7‐9], for instance elevated TG [10] and TC [11]. Previously cohort study indicated that elevated TG was a high risk for diabetes in adults [12, 13]. Akehi et al. [14] also found the strong association between TC and the plasma glucose levels at 60 min. Chakarova et al. [15] examined associations of serum lipid with IFG/IGT in 1240 adults in the population-based cross-sectional study, the results showed that IFG presents with significant differences in the levels of TG and TC are significantly higher compared to the group with normoglycaemia, while TG of IGT group are increased as compared to normoglycaemia, and the differences in TC between IGT and normoglycaemia are not statistically significant.

There are some studies about the association of TG, TC with pathoglycemia in China. Previously two cross-sectional studies [16, 17] examined that elevated TG and TC are risk factors for diabetes in Chinese adults. Multivariate logisticregression analyses of a cross-sectional study involving 4 583Chinese adults reportedhigher TG was therisk factors for developing pre-diabetes [18]. However, so we have known, the association between TG, TC and IFG/IGT is still not fully elucidated at present. Therefore, we designed this study to assess the association between TG, TC and IFG/IGT in general population based on a large population-based cross-sectional survey in Qingdao, China.

In this study, 35.4% (634/1793) men and 35.0% (912/2607) had IFG/IGT. The baseline characteristics of the study population were presented in Table 1. Compared with normal glycaemia, those individuals with IFG/IGT were older, more obese, and have higher levels of systolic blood pressure (SBP), ALT, GGT, TG and TC. Family history of diabetes, unban living and higher income level in men and less school years, lower income level and higher level diastolic blood pressure (DBP) in women with pre-diabetes was more common than in those with normoglycaemia.

In both gender, the level of serum TG was independently and positively associated with FPG and 2 h PG (P < 0.05), and the similar association was between serum TC and FPG, 2 h PG (P < 0.05). The spearman association was shown in Fig. 2.

Table 2 showed OR for IFG/IGT in association to triglycerides and total cholesterol concentration by univariate logistic regression. In both gender, age, BMI, WC, SBP, DBP, ALT and GGT were significantly associated with a higher risk for IFG/IGT (P < 0.05). Unban living and higher income level were significantly associated with a higher risk for IFG/IGT in men(P < 0.05), while married, more school years and higher income level were significantly associated with a lower risk for IFG/IGT in men(P < 0.05). Borderline high TG, HTG and borderline high TC, HTC had a higher OR for IFG/IGT in both men and women (P < 0.05).

Table 3 showed the multivariate adjusted OR between TG, TC and IFG/IGT employing backward method. The multivariate adjusted OR of having IFG/IGT was significantly higher in men with HTG,borderline high TC, HTC, and in women with borderline high TG, HTGthan in those with the normal TG, TC. The association between borderline high TG in men, borderline high TC, HTC in women and IFG/IGT was no significant, but increased risk.Concomitantly, age, SBP, ALT were significant positive association with an increased risk of IFG/IGT in both gender in multivariate logistic model. While, a significant positive association wasdiscovered between IFG/IGT and higher income level, WC in men and urban living in women as well as a significant invert association with more school years, middle income level in women. The results of the multivariable logistic analysis were not changed substantively when BMI taken a place of WC was entered into the models.

The AUROCs of TG, TC for IFG/IGT were relatively smaller (0.50 < AUROC< 0. 7) in both gender. The optimal cut-offs for TG and TC were 1.61 (sensitivity: 0. 34, specificity: 0.77), 4.91 (sensitivity: 0.70, specificity: 0.43) in men and 1. 24 (sensitivity: 0.52, specificity: 0.67), 5. 32 (sensitivity: 0.53, specificity: 0.59) in women, respectively. The AUROCs of TG, TC for pre-diabetes was presented in Table 4 and Fig. 3.

In the current population-based cross-sectional study, spearman analysis revealed that serum TG and TC were independently and positively associated with FPG, 2 h-PG in both men and women. Multivariate logistic analysis showed HTG in both gender,borderline high TG in women, and borderline high TC, HTC in men were positively associated with IFG/IGT independent of other known risk factors. However, AUROC results showed TG and TC as predictor of IFG/IGT was low accuracy.

To the best of our knowledge, serum TG was associated with glucose. Akehi et al. [14] demonstrated that the strong association between TG and plasma glucose levels at 60 min, which hinted TG could be as a predictor for futurediabetes in young males. The cross-sectional and cohort studies of adults all indicated that elevated TG level was a positively associated with diabetic subjects after adjusted for confounding factor [13, 16, 17]. Furthermore, Rasmussen et al. reported that hypertriacylglycerolaemia was significant predictor of progression in IFG/IGT subjects, and decrease of hypertriacylglycerolaemia markedly reduced the risk of diabetes in these high-risk subjects [20]. These findings also agree with our results. In our present multivariable analysis, the associations between TG and IFG/IGT remained constantly considering the confounding factors. Furthermore, previous research demonstrated that evaluated fasting TG could predict future diabetes [21, 22]. However, TG predicting IFG/IGT TG was low accuracy, and the first in Chinese population. The previously follow-up study indicated evaluated TG increase the risk of evaluated glucose [13]. Meanwhile, TG level changes were associated with initiation of changes in lifestyle parameters [23]. The patho-physiological mechanisms of abnormal TC and glucose are intricate and hard to fully understand. TG is responsible for the bidirectional transference of adipose fat and blood glucose from the liver. Previously reported research has confirmed that excessive TG are restructured into adipose fat in liver and stored into hepatocytes [24]. While, superfluous adipose fat can reduce response to insulin signaling [25].

Yet, less research on TC and IFG/IGT has been done at present. Akehi et al. [14] found that TC was significantly associated with plasma glucose levels at 60 min and 120 min in young males. Previously cross-sectional studies [16, 17] revealed that elevated TC increased the risk for diabetes in multivariable logistic models. Meanwhile, the population-based studies [26, 27] reported that TC was a significant association with pre-diabetes after adjusting for multiple covariates. Our present analysiswassimilar but not identical to previous researches. Evaluated TC was significant positive associationin men and insignificant positive association in women with IFG/IGT, yet, couldn’t predict IFG/IGT accurately. Published study has indicated that excess TC may alter the potassium channels of beta-cells, which reduce the activity of glucokinase, and then affect insulin signaling reducing the metabolism of glucose [28].

The present study demonstrated that IFG/IGT was significant positively associated with age, urban living, WC and SBP, which were similar to published studies [18, 29, 30]. Abnormal glucose, which brings on diabetic microangiopathy, is more likely to occur as a consequence of ageing, elevated WC, and urbanization. Microangiopathy causes other series of physiological dysfunction, such as hypertension. Of note, the inverse association between and abnormal glucose had been revealed in previous and present studies [31, 32]. The individual with higher educational attainment are more interested in health. Nevertheless, the effect of personal monthly income on glucose is the controversial issues in present study, and previously studies also showeda mixed opinion on income [33, 34]. Perhaps,men with higher income have greater living and mental pressure and unhealthy lifestyle. However, women with higher income pay more attention to health in China.So, educational initiatives for individuals with abnormal glucose are necessary by present results.

As far as we know, this is the first study to investigate the association between TG, TC level and IFG/IGT in China. In addition, the present study has some strengths. Firstly, this is the relatively large number of subjects included in this population-based study to provide the association between TG, TC and IFG/IGT with high statistical power for data analyses. Secondly, all IFG/IGT subjects were defined according to FPG and/or 2 h PG, which was accepted standard criteria in current. Thirdly, we have fully captured multiple statistical models to investigate the strength of the association between TG, TC level and IFG/IGT in different populations and different types of variables. However, our study suffered from a few potential weaknesses that merit comment. The current study was from two cross-sectional studies, it is enormous challenges to evaluate the association between TG, TC and IFG/IGT. Secondly, only PFC, 2 h PG, TG and TC were performed once in the current study.

Evaluated TG and TC has emphasized significantly positively associated with IFG/IGT. HTG, borderline high TC, HTC in men and borderline high TG, HTG in women had anextremelyadverse impacton IFG/IGT, however, TG and TC could not be anauthenticpredictors of IFG/IGT in this Chinese population. Additionally, further research about the association between TC, TG concentration and hyperglycemia should be conduct large-scale, multi-population-based study to confirm the conclusion in Chinese population.