Background
Methods
Materials
Animal Treatment and Care
Sample collection
Histologic analysis
Blood biochemical marker assays
Measurement of total lipid, triglyceride, and cholesterol levels
Analyses of lipid peroxidation
Measurement of glutathione
Sirius red collagen staining
Statistical analysis
Results
Validation of the HPLC method
Linearity
Precision and accuracy
Present (g/100 g) | Added (g/100 g) | Found (g/100 g) | Accuracy (%) | Precision (RSD%) | |
---|---|---|---|---|---|
Intra-day | |||||
Bisdemethoxycurcumin | 0.095 | 0.096 | 0.097 ± 0.0001 | 101.08 | 0.13 |
Demethoxycurcumin | 0.247 | 0.262 | 0.257 ± 0.0003 | 98.15 | 0.10 |
Curcumin | 0.993 | 1.066 | 1.022 ± 0.002 | 95.84 | 0.13 |
Inter-day | |||||
Bisdemethoxycurcumin | 0.095 | 0.096 | 0.098 ± 0.001 | 101.74 | 0.59 |
Demethoxycurcumin | 0.247 | 0.262 | 0.257 ± 0.001 | 97.96 | 0.48 |
Curcumin | 0.993 | 1.066 | 1.021 ± 0.003 | 95.78 | 0.30 |
Analysis of compounds in turmeric extract
Component | mg/100 g |
---|---|
Curcumin | 901.6 ± 5.4 |
Curcuminoids | 1244.8 ± 3.9 |
BDMC | 108.3 ± 2.9 |
DMC | 234.9 ± 1.9 |
Turmeric extract and its active compound, curcumin, regulate body weight during chronic CCl4 exposure
Group | Food intake (g/day) | Body weight (g) |
---|---|---|
Control | 22.1 ± 3.1 | 335 ± 11.2 |
Curcumin | 23.2 ± 3.3 | 335 ± 10.5 |
300 mg/kg turmeric extract | 22.0 ± 2.3 | 351 ± 12.2 |
CCl4 | 22.3 ± 3.2 | 401 ± 20.4 |
CCl4 + curcumin | 19.9 ± 3.2 | 376.0 ± 18.5 |
CCl4 + 100 mg/kg turmeric extract | 24.6 ± 2.0 | 400.4 ± 19.5 |
CCl4 + 200 mg/kg turmeric extract | 23.1 ± 1.3 | 381.1 ± 20.3* |
CCl4 + 300 mg/kg turmeric extract | 21.2 ± 2.1 | 362.5 ± 20.2* |
Both turmeric extract and curcumin regulate CCl4-induced lipid accumulation
Group | Serum levels (mg/dl) | |||
---|---|---|---|---|
TG | Total cholesterol | LDL-cholesterol | HDL-cholesterol | |
Control | 82.4 ± 2.2 | 136.7 ± 12.8 | 56.6 ± 6.3 | 45.4 ± 6.4 |
Curcumin | 89.4 ± 1.2 | 142.7 ± 6.3 | 60.2 ± 3.1 | 45.6 ± 1.6 |
300 mg/kg turmeric extract | 83.7 ± 2.3 | 141.9 ± 1.5 | 63.2 ± 2.0 | 46.5 ± 3.3 |
CCl4 | 51.5 ± 7.1 | 94.3 ± 1.7 | 44.1 ± 4.0 | 44.5 ± 2.5 |
CCl4 + curcumin | 77.0 ± 1.6 | 111.6 ± 8.3 | 52.2 ± 6.0 | 45.0 ± 9.4 |
CCl4 + 100 mg/kg turmeric extract | 75.1 ± 1.0 | 95.2 ± 9.4 | 46.6 ± 7.1 | 43.8 ± 1.8 |
CCl4 + 200 mg/kg turmeric extract | 78.1 ± 2.5* | 101.3 ± 3.6* | 49.6 ± 3.1* | 45.4 ± 1.5 |
CCl4 + 300 mg/kg turmeric extract | 83.0 ± 1.5* | 112.3 ± 9.1* | 51.5 ± 8.0* | 44.8 ± 2.6 |
Both turmeric extract and curcumin protect against CCl4-induced liver damage
Turmeric extract and curcumin regulate CCl4-induced lipid peroxidation and antioxidant activity
Turmeric extract and curcumin regulate CCl4-induced oxidative stress in the liver
Discussion
Group | Liver levels (mg/dl) | |||
---|---|---|---|---|
TG | Total cholesterol | LDL-cholesterol | HDL-cholesterol | |
Control | 60.6 ± 0.1 | 70.6 ± 5.2 | 50.5 ± 4.3 | 44.3 ± 2.5 |
Curcumin | 68.1 ± 0.1 | 68.9 ± 1.3 | 48.5 ± 8.5 | 47.1 ± 4.6 |
300 mg/kg turmeric extract | 61.3 ± 0.2 | 71.3 ± 1.6 | 45.8 ± 5.5 | 45.6 ± 1.3 |
CCl4 | 92.7 ± 0.2 | 99.6 ± 1.5 | 84.6 ± 7.6 | 44.9 ± 7.8 |
CCl4 + curcumin | 78.9 ± 0.2* | 75.4 ± 1.5* | 62.5 ± 5.6* | 46.2 ± 1.5 |
CCl4 + 100 mg/kg turmeric extract | 87.1 ± 0.1 | 92.7 ± 2.0 | 82.2 ± 5.5 | 45.6 ± 5.6 |
CCl4 + 200 mg/kg turmeric extract | 77.3 ± 0.1* | 88.3 ± 4.6* | 75.5 ± 7.6* | 45.0 ± 2.8 |
CCl4 + 300 mg/kg turmeric extract | 68.3 ± 0.1* | 78.1 ± 1.4* | 61.5 ± 7.6* | 46.5 ± 1.9 |