Background
Methods
Patients and clinical features
Patients designation | Age | Sex | Site of tumor | Histology | Follow up | ||
---|---|---|---|---|---|---|---|
Metastatic carcinomas originating in the lung
|
Patient1
| T1 | 54 | M | RLL | squamous carcinoma | Alive |
T2 | Sternum | squamous carcinoma | |||||
Patient2
| T1 | 59 | M | Brain | adenocarcinoma | Dead | |
T2 | RUL | adenocarcinoma | |||||
Patient3
| T1 | 70 | M | LUL | squamous carcinoma | Dead | |
T2 | Brain | squamous carcinoma | |||||
Patient4
| T1 | 45 | F | LLL | adenocarcinoma | Dead | |
T2 | Brain | adenocarcinoma | |||||
Patient5
| T1 | 39 | F | RLL | adenocarcinoma | Alive | |
T2 | Brain | adenocarcinoma | |||||
Patient6
| T1 | 58 | F | RUL | adenocarcinoma | Alive | |
T2 | Rib | adenocarcinoma | |||||
Patient7
| T1 | 56 | M | Brain | adenocarcinoma | Dead | |
T2 | RML | adenocarcinoma | |||||
Patient8
| T1 | 55 | M | LUL | squamous carcinoma | Alive | |
T2 | Left adrenal gland | squamous carcinoma | |||||
Patient9
| T1 | 46 | M | LLL | adenocarcinoma | Alive | |
T2 | Brain | adenocarcinoma | |||||
Patient10
| T1 | 56 | M | LUL | squamous carcinoma | Alive | |
T2 | Brain | squamous carcinoma | |||||
Synchronous lung tumor with different histological types
|
Patient1
| T1 | 71 | M | RUL | adenocarcinoma | Dead |
T2 | RLL | squamous carcinoma | |||||
Patient2
| T1 | 47 | M | RLL | squamous carcinoma | Alive | |
T2 | RUL | adenocarcinoma | |||||
Patient3
| T1 | 72 | M | LLL | adenocarcinoma | Dead | |
T2 | LUL | squamous carcinoma | |||||
Patient4
| T1 | 59 | M | RUL | adenocarcinoma | Alive | |
T2 | RLL | squamous carcinoma | |||||
Patient5
| T1 | 74 | M | RUL | adenocarcinoma | Alive | |
T2 | RLL | squamous carcinoma | |||||
Synchronous lung tumor with same histological types
|
Patient1
| T1 | 53 | M | RML | adenocarcinoma | Dead |
T2 | RUL | adenocarcinoma | |||||
Patient2
| T1 | 47 | M | LLL | adenocarcinoma | Dead | |
T2 | LUL | adenocarcinoma | |||||
Patient3
| T1 | 52 | F | RUL | adenocarcinoma | Alive | |
T2 | RLL | adenocarcinoma | |||||
Patient4
| T1 | 70 | F | RLL | adenocarcinoma | Alive | |
T2 | RUL | adenocarcinoma | |||||
Patient5
| T1 | 73 | M | LLL | squamous carcinoma | Alive | |
T2 | LUL | squamous carcinoma | |||||
Patient6
| T1 | 65 | M | RML | squamous carcinoma | Alive | |
T2 | RLL | squamous carcinoma | |||||
Patient7
| T1 | 55 | M | LLL | squamous carcinoma | Alive | |
T2 | LUL | squamous carcinoma | |||||
Patient8
| T1 | 69 | M | RUL | adenocarcinoma | Dead | |
T2 | RLL | adenocarcinoma |
Preparation of genomic DNA
Microsatellite PCR analysis
Results
Analysis of primary and metastatic tumors
Patient designation | D2S1363 | D6S1056 | D7S1824 | D10S1239 | D15S822 | D22S689 | ||
---|---|---|---|---|---|---|---|---|
Primary and metastatic tumors
|
Patient1
| T1 |
+
|
+
|
+
|
+
|
+
|
-
|
T2 |
-
|
+
|
+
|
-
|
-
|
-
| ||
Patient2
| T1 |
-
|
-
|
-
|
-
|
++
|
-
| |
T2 |
-
|
+
|
+
|
+
|
++
|
-
| ||
Patient3
| T1 |
+
|
++
|
+
|
++
|
+
|
+
| |
T2 |
+
|
++
|
+
|
+
|
+
|
-
| ||
Patient4
| T1 |
+
|
+
|
+
|
++
|
+
|
-
| |
T2 |
+
|
+
|
+
|
-
|
-
|
-
| ||
Patient5
| T1 |
+
|
+
|
+
|
+
|
++
|
++
| |
T2 |
+
|
+
|
+
|
+
|
++
|
-
| ||
Patient6
| T1 |
++
|
+
|
+
|
++
|
++
|
+
| |
T2 |
+
|
-
|
+
|
-
|
-
|
-
| ||
Patient7
| T1 |
-
|
-
|
-
|
+
|
-
|
-
| |
T2 |
++
|
-
|
+
|
++
|
-
|
-
| ||
Patient8
| T1 |
+
|
++
|
+
|
+
|
+
|
-
| |
T2 |
+
|
-
|
+
|
+
|
-
|
-
| ||
Patient9
| T1 |
+
|
++
|
+
|
++
|
+
|
-
| |
T2 |
+
|
++
|
+
|
++
|
-
|
-
| ||
Patient10
| T1 |
++
|
++
|
+
|
+
|
-
|
-
| |
T2 |
-
|
-
|
+
|
+
|
-
|
-
| ||
Synchronous tumors with different histological types
|
Patient1
| T1 |
+
|
+
|
+
|
-
|
-
|
+
|
T2 |
+
|
+
|
+
|
++
|
-
|
-
| ||
Patient2
| T1 |
++
|
++
|
+
|
++
|
++
|
-
| |
T2 |
++
|
+++
|
+
|
+
|
++
|
-
| ||
Patient3
| T1 |
++
|
-
|
+
|
-
|
++
|
-
| |
T2 |
-
|
+
|
-
|
+
|
++
|
-
| ||
Patient4
| T1 |
-
|
-
|
+
|
+
|
-
|
-
| |
T2 |
++
|
-
|
+
|
-
|
-
|
-
| ||
Patient5
| T1 |
++
|
++
|
+
|
++
|
+
|
+
| |
T2 |
++
|
+
|
+
|
++
|
++
|
+
| ||
Synchronous tumors with same histological types
|
Patient1
| T1 |
+
|
++
|
+
|
++
|
+
|
+
|
T2 |
+
|
+
|
+
|
++
|
-
|
-
| ||
Patient2
| T1 |
++
|
+
|
+
|
+
|
++
|
+
| |
T2 |
+
|
-
|
+
|
+
|
-
|
-
| ||
Patient3
| T1 |
++
|
++
|
+
|
++
|
++
|
+
| |
T2 |
++
|
++
|
+
|
++
|
++
|
-
| ||
Patient4
| T1 |
++
|
-
|
+
|
-
|
-
|
-
| |
T2 |
-
|
-
|
+
|
++
|
-
|
-
| ||
Patient5
| T1 |
+
|
+
|
+
|
+
|
-
|
-
| |
T2 |
+
|
+
|
-
|
+
|
++
|
+
| ||
Patient6
| T1 |
+
|
+++
|
+
|
+
|
-
|
-
| |
T2 |
+
|
+
|
+
|
-
|
-
|
-
| ||
Patient7
| T1 |
-
|
++
|
+
|
+
|
-
|
-
| |
T2 |
-
|
-
|
+
|
+
|
-
|
-
| ||
Patient8
| T1 |
+
|
+
|
+
|
+
|
-
|
-
| |
T2 |
+
|
-
|
+
|
-
|
-
|
-
|