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01.12.2012 | Research | Ausgabe 1/2012 Open Access

World Journal of Surgical Oncology 1/2012

Up-regulated oncoprotein P28GANK correlates with proliferation and poor prognosis of human glioma

Zeitschrift:
World Journal of Surgical Oncology > Ausgabe 1/2012
Autoren:
Yang Yang, Chunli Zhang, Li Li, Yusong Gao, Xinming Luo, Yadong Zhang, Weiping Liu, Zhou Fei
Wichtige Hinweise

Electronic supplementary material

The online version of this article (doi:10.​1186/​1477-7819-10-169) contains supplementary material, which is available to authorized users.

Competing interests

The authors declare that they have no competing interests.

Authors’ contributions

YY carried out the molecular genetic studies, and drafted the manuscript; Chunli Zhang carried out IHC and the analysis of IHC; YG carried out the animal study; ZF carried out the experiment design; XL participated in IHC; YZ carried out the experiment design; BH participated in the sequence alignment. LL carried out the experiment design, and drafted the manuscript. WL carried out the experiment design, and drafted the manuscript. All authors read and approved the final manuscript.

Abstract

Background

The significance of p28GANK in gliomas remains unknown. This study aims to clarify the clinical significance of p28GANK in human gliomas.

Methods

The expression of p28GANK in 138 gliomas and 50 matched para-cancerous tissues was detected by immunohistochemical staining, and statistical analyses were performed to test the correlation of p28GANK with clinical parameters. To investigate the effects of p28GANK down-regulation on the growth of cells both in vitro and in vivo, an siRNA targeting p28GANK was transfected into U251 cells.

Results

P28GANK expression was significantly higher in tumor specimens than in matched para-cancerous tissues. Over-expressed p28GANK significantly correlated with high karnofsky performance score (KPS), advanced WHO grade and poor overall survival of the patients. Univariate analysis showed that WHO grade and KPS also correlated with the survival of patients, and multivariate analysis suggested that KPS and p28GANK expression were two independent prognostic factors. Moreover, p28GANK gene silencing decreased the malignant growth of U251 cells both in vitro and in vivo.

Conclusions

Increased expression of p28GANK is correlated with poor clinical outcomes in glioma patients. The down-regulation of p28GANK significantly inhibited cell proliferation, indicating that p28GANK might be a potential therapeutic target for glioma treatment.
Zusatzmaterial
Authors’ original file for figure 1
12957_2012_1037_MOESM1_ESM.tiff
Authors’ original file for figure 2
12957_2012_1037_MOESM2_ESM.tiff
Literatur
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