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Erschienen in: Archives of Virology 12/2019

23.09.2019 | Brief Report

Validation of a real-time PCR assay for detection of swinepox virus

verfasst von: Felipe Augusto Souza, Erlânio Marcelo dos Santos Júnior, Mateus Laguardia-Nascimento, Tânia Rosária Pereira Freitas, Clarissa R. Damaso, Anselmo V. Rivetti Júnior, Marcelo Fernandes Camargos, Antônio Augusto Fonseca Júnior

Erschienen in: Archives of Virology | Ausgabe 12/2019

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Abstract

Swine are the only known hosts of swinepox virus (SWPV), the sole member of the genus Suipoxvirus, family Poxviridae. Rapid diagnosis is recommended for appropriate interventions because of the high morbidity associated with this virus. This study describes a real-time quantitative PCR (qPCR) assay for rapid detection and quantification of SWPV. The detection limit, repeatability, reproducibility, and specificity of this assay were determined. The efficiency was 96%, and the R2 value was 0.996. The detection limit was 1 fg or 10−0.5 TCID50/50 μL. Tests showed that the greatest source of error in the SWPV qPCR assay was variation between analysts rather than different qPCR kits or equipment. All nucleic acids from other viruses or samples collected from swine were negative in the specificity test. qPCR for SWPV is a new method with tested variables that allows main sources of error in laboratory diagnosis and viral quantification to be identified.
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Metadaten
Titel
Validation of a real-time PCR assay for detection of swinepox virus
verfasst von
Felipe Augusto Souza
Erlânio Marcelo dos Santos Júnior
Mateus Laguardia-Nascimento
Tânia Rosária Pereira Freitas
Clarissa R. Damaso
Anselmo V. Rivetti Júnior
Marcelo Fernandes Camargos
Antônio Augusto Fonseca Júnior
Publikationsdatum
23.09.2019
Verlag
Springer Vienna
Erschienen in
Archives of Virology / Ausgabe 12/2019
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-019-04403-w

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