Arterial compliance, the tendency of blood vessels to stretch in response to the pulsatile blood flow, has significant physiological effects on blood pressure (BP) [1‐3]. The cushioning function of the aorta may be impaired due to structural degeneration of the aortic wall, increasing stiffness and thus the afterload for the left ventricle [3].

Aortic distensibility (AD) provides an estimation of these elastic properties and normalizes arterial compliance with the size of the vessel, allowing for a better comparison between individuals [4].

Previously, several authors have made significant contributions to assess aortic compliance with various imaging methods [5‐8]. Measurements of AD can give information even on subclinical vascular changes and are being investigated as predictors of cardiovascular morbidity [4, 9‐11]. Newer preclinical and clinical studies are focusing on mechanisms of influencing AD. Moreover, we recently showed AD to be a potential biomarker to act as a non-invasive control in interventional hypertension trials [4].

Currently, cardiac magnetic resonance tomography (CMR) is regarded as a gold standard to assess areas of different parts of the aorta to calculate AD. However, some aspects regarding the meaningfulness and interpretability of AD in the descending aorta remain unresolved. Even though the descending aorta is incidentally imaged in every standard 4-chamber view cine steady-state free precession sequence (SSFP) of the heart, it remains uncertain whether these images can be used to calculate AD in daily clinical practice adequately. The current study aimed to systematically analyze reproducibility (with intra- and inter-observer agreement) of cine imaging in the evaluation of AD. Our measurements were acquired using various types of angulation of the descending aorta in CMR.

We performed CMR in 31 subjects (mean age 57 years) with variable indications. The study complies with the Declaration of Helsinki. Institutional Review Board approval was not necessary due to a retrospective analysis of clinical data. According to local law, all individuals signed informed consent before entering the clinical MRI. The data were anonymized and none of the observers had the possibility of identifying patient information when analyzing the data. All images were acquired using a Philips Ingenia 3.0 Tesla Scanner (Philips Healthcare, Best, the Netherlands). Cine images were acquired during breath holds of 10–15 s using vector electrocardiogram gating and steady-state free precession sequence [4]. All study participants were scanned using the same imaging protocol, which consisted of angulation of the descending aorta to obtain strictly transversally and orthogonally cut cross-sectional areas of the aorta [16]. Fifty images per cardiac cycle were obtained. AD was determined as the change in the cross-sectional aortic area per unit change in BP, as previously reported [4, 10, 16, 17]. Office BP was obtained during the MRI with an automatic brachial oscillometric monitor after at least 5 min of rest [4]. Two experienced observers then performed post-processing of the CMR dataset with the Medis Suite Version 2.1. (Medis, Leiden, The Netherlands). Both observers had more than 2 years of experience with general CMR imaging. Qmass software was used to contour the inner diameter of the aortic wall. Maximum and minimum aortic areas were calculated by (i) tracing the largest and smallest extension of the aortic wall contour throughout the cardiac cycle and (ii) tracing aortic diameters to calculate a hypothetic circle aiming to obtain a strictly circular aortic area. We first assessed cross-sectional areas of the descending aorta obtained in standard 4-chamber cine images. In a second step, we performed the same measurements in images based on strictly transversal or orthogonal cuts. Figure 1 illustrates the three different angles used at the time of image acquisition. All measurements were repeated three times and then averaged. To calculate AD, we first determined aortic strain, defined as the relative change in area, and then normalized this value with the peripheral pulse pressure (PP) obtained at the time point of the CMR (average of three PP values). The formula used for the AD calculation has been published previously [4, 10, 16, 17].

The present work was designed to investigate the reproducibility of AD calculation in standard 4-chamber cine CMR imaging. Our data demonstrate the following:

Relationships of pulse pressure (PP) and flow are illustrated by impedance curves, which show higher frequencies when the aortic arch becomes stiffer and then reflects pulse waves earlier [21]. While determinants of peripheral resistance are simple to acquire, parameters of aortic stiffness require consideration of the distending PP, vascular tone and site of measurement [3]. As a gold standard for arterial stiffness, PWV requires certain geometric assumptions and extended planning and remains time consuming. Recently, 4D flow CMR has been shown to directly assess PWV in reduced time and to have a high correlation with AD values [22]. Measures of AD are relatively simple to obtain and reflect alterations in the central vasculature even in the absence of overt cardiovascular disease. No additional scans are needed to calculate AD. Thus, focusing on AD as a novel imaging biomarker for the prediction of cardiovascular risk has significant potential to improve individually adapted therapies [23].

While previous research aimed to assess AD in vivo, newer studies focus on therapeutic concepts to reduce arterial stiffness [11‐14]. Both medical control of heart rate and modulation of the sympathetic nervous system by renal denervation have shown promising results to improve AD. [12‐15] In preclinical studies, heart rate reduction with ivabradine has shown to improve arterial stiffness and diastolic function [14, 15]. Targeting AD might thus play an essential role in the management of heart failure with preserved ejection fraction (HFPEF).

Reproducibility and accuracy of CMR were previously shown to be high, especially compared to those of echocardiography [24, 25]. We assumed that AD estimation would be feasible when focusing on images of the descending aorta that are available in every standard 4-chamber cine sequence of the heart (Fig. 1) [19]. For this purpose, we retrospectively analyzed already existing CMR data. Images were taken in diameter and area. Resulting values differed by about 15% (Fig. 4).

Based on the outlined data, we can assume that AD taken in standard 4-chamber cine images corresponds well with strictly orthogonal images but tends to overestimate values by about 25% (Table 3, Fig. 4). This deviation is a consequence of the geometry of cross-sectional vessel areas displayed with different angulations. While orthogonally derived areas are nearly circular, the areas in the 4-chamber view are based on an oblique section of the vessel in this projection. Values of inter- and intra-observer agreement were excellent in our data, underlining the high potential for retrospective analyses (Fig. 3). To give an impression of how this measurement technique will perform in daily practice, the correlation curves of two different observers are shown in Fig. 2.

CMR was shown to provide highly accurate and reproducible measures, especially when values are derived three times [18, 19, 26]. We used this strategy and averaged three measurements to improve reproducibility. Given the above evidence, AD obtained by tracing of the aortic wall is justified to be used in daily clinical practice for risk prediction.

Recently, two studies investigated AD measurement as a non-invasive tool to control the effect of renal denervation [4, 11]. These studies included 58 and 28 patients, respectively. Based on the present work, it seems that even fewer patients are necessary to detect small changes in AD when using conventional 4-chamber cine CMR images. The sample size calculation given in Table 5 shows that, e.g., 25 exams (n = 19 plus 25% dropout) per arm are necessary to detect a difference of 0.8 10⁻³ mmHg⁻¹ in AD with a power of 90%. This makes our technique especially suitable for retrospective analyses of CMR datasets.

AD measurements using conventional 4-chamber cine imaging are feasible and highly reproducible and reflect the elastic properties of the descending aorta accordingly. This allows an accurate and rapid assessment of arterial compliance and may help to predict changes in the central vasculature at an early stage, eventually preventing evolution toward left ventricular remodeling and dysfunction. Using automated contouring and 3D acquisition of the aorta, future trials could assess AD among different segments to increase accuracy and diagnostic value. In addition, our approach might be even used in a retrospective analysis of studies on already existing CMR data.

Among the major limits of our study is the relatively small number of patients included. A larger cohort could enhance the power of our results and outline more subtle differences among different angulations. We used a standard CMR cine sequence; the effect of different temporal and spatial resolutions of SSFP cine sequences on AD measurements has not been evaluated. There is a lack of comparison of our results with other markers of arterial compliance (e.g., PWV) and the absence of centrally measured BP. The invasiveness would, however, have limited the conduct of this study. Another limitation is the use of peripheral PP in our investigation. Central aortic PP would provide a more accurate absolute measure of AD.