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01.12.2014 | Research | Ausgabe 1/2014 Open Access

Gut Pathogens 1/2014

Variations in motility and biofilm formation of Salmonella enterica serovar Typhi

Zeitschrift:
Gut Pathogens > Ausgabe 1/2014
Autoren:
Kalaivani Kalai Chelvam, Lay Ching Chai, Kwai Lin Thong
Wichtige Hinweise

Electronic supplementary material

The online version of this article (doi:10.​1186/​1757-4749-6-2) contains supplementary material, which is available to authorized users.

Competing interests

The author’s declare that they have no competing interests.

Authors’ contributions

KK performed the motility assays, biofilm assays and SEM assays on Salmonella Typhi, analysed the data, performed statistical analyses and drafted the manuscript. LCC supervised all experimental work, analysed the data, and drafted the manuscript. KLT conceived the study, supervised all experimental work, analysed the data and drafted the manuscript. All authors read and approved the final manuscript.

Abstract

Background

Salmonella enterica serovar Typhi (S. Typhi) exhibits unique characteristics as an intracellular human pathogen. It causes both acute and chronic infection with various disease manifestations in the human host only. The principal factors underlying the unique lifestyle of motility and biofilm forming ability of S. Typhi remain largely unknown. The main objective of this study was to explore and investigate the motility and biofilm forming behaviour among S. Typhi strains of diverse background.

Results

Swim and swarm motility tests were performed with 0.25% and 0.5% agar concentration, respectively; while biofilm formation was determined by growing the bacterial cultures for 48 hrs in 96-well microtitre plate. While all S. Typhi strains demonstrated swarming motility with smooth featureless morphology, 58 out of 60 strains demonstrated swimming motility with featureless or bull’s eye morphology. Interestingly, S. Typhi strains of blood-borne origin exhibited significantly higher swimming motility (P < 0.05) than stool-borne strains suggesting that swimming motility may play a role in the systemic invasion of S. Typhi in the human host. Also, stool-borne S. Typhi displayed a negative relationship between motility and biofilm forming behaviour, which was not observed in the blood-borne strains.

Conclusion

In summary, both swimming and swarming motility are conserved among S. Typhi strains but there was variation for biofilm forming ability. There was no difference observed in this phenotype for S. Typhi strains from diverse background. These findings serve as caveats for future studies to understand the lifestyle and transmission of this pathogen.
Zusatzmaterial
Authors’ original file for figure 1
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Authors’ original file for figure 6
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Literatur
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