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01.04.2013 | Research Article

VEGF-A and VEGF-B mRNA expression in gastro-oesophageal cancers

verfasst von: Cristina Angelescu, Florin Burada, Mihai Ioana, Radu Angelescu, Emil Moraru, Anca Riza, Sanda Marchian, Francisc Mixich, Mihai Cruce, Adrian Săftoiu

Erschienen in: Clinical and Translational Oncology | Ausgabe 4/2013

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Abstract

Introduction

Angiogenesis is essential for the local growth, invasion and metastasis of the tumours. Vascular endothelial growth factors (VEGFs) play a crucial role in tumour angiogenesis. The aim of our study was to quantify the expression of several VEGF family molecules in human gastro-oesophageal cancers and to analyse possible correlations between genes expression and clinico-pathological features.

Materials and methods

Gene expression was quantified in 43 gastro-oesophageal paired samples using qRT-PCR with TaqMan probes specific to VEGF-A, including soluble transcript variants and VEGF-B genes.

Results

VEGF-A, including the studied splice variants and VEGF-B mRNAs were expressed in both tumour and peritumour mucosa. The expression of VEGF-A and its isoforms was higher in tumour compared with paired peritumour mucosa, while no significant difference was observed in VEGF-B expression. VEGF-A expression tended to correlate with tumour invasion.

Conclusion

VEGF-A has a tendency to over-express in gastro-oesophageal cancers, while VEGF-B does not seem involved in these tumours. Further studies are required to establish the utility of anti-VEGF-A therapy and to find biomarkers for pathogenesis or response to therapy in gastro-oesophageal tumours.

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Titel: VEGF-A and VEGF-B mRNA expression in gastro-oesophageal cancers
verfasst von: Cristina Angelescu
Florin Burada
Mihai Ioana
Radu Angelescu
Emil Moraru
Anca Riza
Sanda Marchian
Francisc Mixich
Mihai Cruce
Adrian Săftoiu
Publikationsdatum: 01.04.2013
Verlag: Springer Milan
Erschienen in: Clinical and Translational Oncology / Ausgabe 4/2013
Print ISSN: 1699-048X
Elektronische ISSN: 1699-3055
DOI: https://doi.org/10.1007/s12094-012-0923-y

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VEGF-A and VEGF-B mRNA expression in gastro-oesophageal cancers

Abstract

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Materials and methods

Results

Conclusion

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Abstract

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Materials and methods

Results

Conclusion

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