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Brain Structure and Function

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23.02.2018 | Original Article

Vesicular glutamate transporter 1 (VGLUT1)- and VGLUT2-immunopositive axon terminals on the rat jaw-closing and jaw-opening motoneurons

verfasst von: Sook Kyung Park, Sang Jin Ko, Sang Kyoo Paik, Jong-Cheol Rah, Kea Joo Lee, Yong Chul Bae

Erschienen in: Brain Structure and Function | Ausgabe 5/2018

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Abstract

To provide information on the glutamatergic synapses on the trigeminal motoneurons, which may be important for understanding the mechanism of control of jaw movements, we investigated the distribution of vesicular glutamate transporter (VGLUT)1-immunopositive (+) and VGLUT2 + axon terminals (boutons) on the rat jaw-closing (JC) and jaw-opening (JO) motoneurons, and their morphological determinants of synaptic strength by retrograde tracing, electron microscopic immunohistochemistry, and quantitative ultrastructural analysis. We found that (1) the large majority of VGLUT + boutons on JC and JO motoneurons were VGLUT2+, (2) the density of VGLUT1 + boutons terminating on JC motoneurons was significantly higher than that on JO motoneurons, (3) the density of VGLUT1 + boutons terminating on non-primary dendrites of JC motoneurons was significantly higher than that on somata or primary dendrites, whereas the density of VGLUT2 + boutons was not significantly different between JC and JO motoneurons and among various compartments of the postsynaptic neurons, and (4) the bouton volume, mitochondrial volume, and active zone area of the VGLUT1 + boutons forming synapses on JC motoneurons were significantly bigger than those of VGLUT2 + boutons. These findings suggest that JC and JO motoneurons receive glutamatergic input primarily from VGLUT2-expressing intrinsic neurons (premotoneurons), and may be controlled differently by neurons in the trigeminal mesencephalic nucleus and by glutamatergic premotoneurons.

We used the formula V (µ³) = \({\text{~}}\mathop \sum \nolimits_{{i=1}}^{n} 0.{\text{075A}}i{\text{~}}\) and Area (µ²) =\({\text{~}}\mathop \sum \nolimits_{{i=1}}^{n} 0.{\text{075}}Li{\text{~}}\): “V” is the volume of a bouton or mitochondria, “A” is the cross-sectional area of the bouton or mitochondria of the bouton measured in an individual section. “Area” is the active zone area of a bouton, “L” is length of presynaptic density of the bouton measured in an individual section. “n” is the number of sections in the series.

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Titel: Vesicular glutamate transporter 1 (VGLUT1)- and VGLUT2-immunopositive axon terminals on the rat jaw-closing and jaw-opening motoneurons
verfasst von: Sook Kyung Park
Sang Jin Ko
Sang Kyoo Paik
Jong-Cheol Rah
Kea Joo Lee
Yong Chul Bae
Publikationsdatum: 23.02.2018
Verlag: Springer Berlin Heidelberg
Erschienen in: Brain Structure and Function / Ausgabe 5/2018
Print ISSN: 1863-2653
Elektronische ISSN: 1863-2661
DOI: https://doi.org/10.1007/s00429-018-1636-y

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Vesicular glutamate transporter 1 (VGLUT1)- and VGLUT2-immunopositive axon terminals on the rat jaw-closing and jaw-opening motoneurons

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