Skip to main content
main-content

01.12.2012 | Research | Ausgabe 1/2012 Open Access

Journal of Inflammation 1/2012

Visualizing arthritic inflammation and therapeutic response by fluorine-19 magnetic resonance imaging (19F MRI)

Zeitschrift:
Journal of Inflammation > Ausgabe 1/2012
Autoren:
Anthony Balducci, Brooke M Helfer, Eric T Ahrens, Charles F O’Hanlon III, Amy K Wesa
Wichtige Hinweise

Electronic supplementary material

The online version of this article (doi:10.​1186/​1476-9255-9-24) contains supplementary material, which is available to authorized users.

Competing interests

The authors, except ETA, are employees of Celsense, Inc. and receive salary and stock options. ETA serves as a paid consultant to Celsense and is a stock holder.

Authors’ contributions

AB contributed to the design of the studies, production of the contrast agent, and contributed to analysis of the MRI data, including rendering 3D images, and quantification. AB was involved in the interpretation of data and revision of manuscript. BMH contributed to the design and analysis of the studies, assessment of the contrast agent and also was involved in drafting and revising the manuscript. ETA assisted with design and aided in preparation of the manuscript. AKW and CFO were responsible for the conception and planning of the experiments, and CFO helped to revise the manuscript. AKW directed the study, designed the experiments, contributed to the analysis and interpretation of the data, and drafted the manuscript. All authors have read and approved the final version of this manuscript.

Abstract

Background

Non-invasive imaging of inflammation to measure the progression of autoimmune diseases, such as rheumatoid arthritis (RA), and to monitor responses to therapy is critically needed. V-Sense, a perfluorocarbon (PFC) contrast agent that preferentially labels inflammatory cells, which are then recruited out of systemic circulation to sites of inflammation, enables detection by 19F MRI. With no 19F background in the host, detection is highly-specific and can act as a proxy biomarker of the degree of inflammation present.

Methods

Collagen-induced arthritis in rats, a model with many similarities to human RA, was used to study the ability of the PFC contrast agent to reveal the accumulation of inflammation over time using 19F MRI. Disease progression in the rat hind limbs was monitored by caliper measurements and 19F MRI on days 15, 22 and 29, including the height of clinically symptomatic disease. Naïve rats served as controls. The capacity of the PFC contrast agent and 19F MRI to assess the effectiveness of therapy was studied in a cohort of rats administered oral prednisolone on days 14 to 28.

Results

Quantification of 19F signal measured by MRI in affected limbs was linearly correlated with disease severity. In animals with progressive disease, increases in 19F signal reflected the ongoing recruitment of inflammatory cells to the site, while no increase in 19F signal was observed in animals receiving treatment which resulted in clinical resolution of disease.

Conclusion

These results indicate that 19F MRI may be used to quantitatively and qualitatively evaluate longitudinal responses to a therapeutic regimen, while additionally revealing the recruitment of monocytic cells involved in the inflammatory process to the anatomical site. This study may support the use of 19F MRI to clinically quantify and monitor the severity of inflammation, and to assess the effectiveness of treatments in RA and other diseases with an inflammatory component.
Zusatzmaterial
Additional file 1:Table S1. Longitudinal analysis of 19F signal in CIA rats. (PDF 336 KB)
12950_2011_248_MOESM1_ESM.pdf
Authors’ original file for figure 1
12950_2011_248_MOESM2_ESM.pdf
Authors’ original file for figure 2
12950_2011_248_MOESM3_ESM.tiff
Authors’ original file for figure 3
12950_2011_248_MOESM4_ESM.pdf
Authors’ original file for figure 4
12950_2011_248_MOESM5_ESM.pdf
Authors’ original file for figure 5
12950_2011_248_MOESM6_ESM.pdf
Authors’ original file for figure 6
12950_2011_248_MOESM7_ESM.pdf
Authors’ original file for figure 7
12950_2011_248_MOESM8_ESM.tiff
Authors’ original file for figure 8
12950_2011_248_MOESM9_ESM.tiff
Literatur
Über diesen Artikel

Weitere Artikel der Ausgabe 1/2012

Journal of Inflammation 1/2012 Zur Ausgabe

Neu im Fachgebiet Innere Medizin


 

Meistgelesene Bücher aus der Inneren Medizin

2017 | Buch

Rheumatologie aus der Praxis

Entzündliche Gelenkerkrankungen – mit Fallbeispielen

Dieses Fachbuch macht mit den wichtigsten chronisch entzündlichen Gelenk- und Wirbelsäulenerkrankungen vertraut. Anhand von über 40 instruktiven Fallbeispielen werden anschaulich diagnostisches Vorgehen, therapeutisches Ansprechen und der Verlauf …

Herausgeber:
Rudolf Puchner

2016 | Buch

Ambulant erworbene Pneumonie

Was, wann, warum – Dieses Buch bietet differenzierte Diagnostik und Therapie der ambulant erworbenen Pneumonie zur sofortigen sicheren Anwendung. Entsprechend der neuesten Studien und Leitlinien aller wichtigen Fachgesellschaften.

Herausgeber:
Santiago Ewig

Mail Icon II Newsletter

Bestellen Sie unseren kostenlosen Newsletter Update Innere Medizin und bleiben Sie gut informiert – ganz bequem per eMail.

© Springer Medizin 

Bildnachweise