Skip to main content
main-content
Erschienen in: Der Ophthalmologe 10/2009

01.10.2009 | Originalien

Vitreal induzierte RPE-Zell-Traktion

Untersuchung pathologischer Glaskörperproben in einem In-vitro-Kontraktionsmodell

verfasst von: J. Beutel, M. Lüke, K.-U. Bartz-Schmidt, Prof. S. Grisanti

Erschienen in: Die Ophthalmologie | Ausgabe 10/2009

Einloggen, um Zugang zu erhalten

Zusammenfassung

Hintergrund

Zielsetzung dieser Studie war, die Kontraktilität von retinalen Pigmentepithel- (RPE-)Zellen zu quantifizieren, die durch bioaktive Faktoren pathologischer Glaskörperproben induziert wird.

Material und Methoden

Unter Verwendung eines In-vitro-Kontraktionsmodells konnten die Aktivität von Glaskörperproben unterschiedlicher vitreoretinaler Erkrankungen untersucht werden. Dazu wurden transdifferentierte porcine RPE-Zellen auf hemisphärische Typ-I-Kollagen-Gele aufgebracht. Nach Zugabe der Glaskörperproben [physiologisch (n=6); rhegmatogene Ablatio (n=11); proliferative Vitreoretinopathie (PVR, n=10); proliferative diabetische Retinopathie (PDR, n=6)] wurde die induzierte Gelkontraktion bestimmt.

Ergebnisse

Die spezifische Aktivität der unklassifizierten Proben betrug 0,04 (Median, Spannweite, SW: 0–0,08). Glaskörperproben, die von Patienten mit verschiedenen PVR-Stadien gewonnen wurden, wiesen eine spezifische Aktivität von 0,45 (Median, SW: 0,03–1,45) auf. Die Glaskörperproben von rhegmatogen bedingten Netzhautablösungen zeigten eine Aktivität von 0,13 (Median, SW: 0,01–0,93). Die spezifische Aktivität von Glaskörperproben von Patienten mit diabetischer Retinopathie betrug 0,17 (Median, SW: 0,06–0,29). Die mittlere spezifische Aktivität und Gesamtaktivität dieser Gruppen waren signifikant im Vergleich zu den unklassifizierten Proben bzw. den Basiswerten erhöht (p<0,05).

Schlussfolgerung

Pathologische Glaskörperproben unterschiedlicher vitreoretinaler Erkrankungen enthalten ausreichende Mengen von biologisch aktiven Substanzen, die eine Kontraktion von Extrazellulärmatrix induzieren können.
Literatur
1.
Zurück zum Zitat Baudouin C, Fredj-Reygrobellet D, Brignole F et al. (1993) Growth factors in vitreous and subretinal fluid cells from patients with proliferative vitreoretinopathy. Ophthalmic Res 25: 52–59 PubMedCrossRef Baudouin C, Fredj-Reygrobellet D, Brignole F et al. (1993) Growth factors in vitreous and subretinal fluid cells from patients with proliferative vitreoretinopathy. Ophthalmic Res 25: 52–59 PubMedCrossRef
2.
Zurück zum Zitat Charteris DG (1995) Proliferative vitreoretinopathy: pathobiology, surgical management and adjunctive treatment. Br J Ophthalmol 79: 953–960 CrossRefPubMed Charteris DG (1995) Proliferative vitreoretinopathy: pathobiology, surgical management and adjunctive treatment. Br J Ophthalmol 79: 953–960 CrossRefPubMed
3.
Zurück zum Zitat Connor TB jr, Roberts AB, Sporn MB et al. (1989) Correlation of fibrosis and transforming growth factor-beta type 2 levels in the eye. J Clin Invest 83: 1661–1666 CrossRefPubMed Connor TB jr, Roberts AB, Sporn MB et al. (1989) Correlation of fibrosis and transforming growth factor-beta type 2 levels in the eye. J Clin Invest 83: 1661–1666 CrossRefPubMed
4.
Zurück zum Zitat Das A, McGuire PG, Eriqat C et al. (1999) Human diabetic neovascular membranes contain high levels of urokinase and metalloproteinase enzymes. Invest Ophthalmol Vis Sci 40: 809–813 PubMed Das A, McGuire PG, Eriqat C et al. (1999) Human diabetic neovascular membranes contain high levels of urokinase and metalloproteinase enzymes. Invest Ophthalmol Vis Sci 40: 809–813 PubMed
5.
Zurück zum Zitat Flood M, Gouras P, Kjeldbye H (1980) Growth characteristics and ultrastructure of human retinal pigment epithelium in vitro. Invest Ophthalmol Vis Sci 19: 1309–1320 PubMed Flood M, Gouras P, Kjeldbye H (1980) Growth characteristics and ultrastructure of human retinal pigment epithelium in vitro. Invest Ophthalmol Vis Sci 19: 1309–1320 PubMed
6.
Zurück zum Zitat Grisanti S, Guidry C (1995) Transdifferentiation of retinal pigment epithelial cells from epithelial to mesenchymal phenotype. Invest Ophthalmol Vis Sci 36: 391–405 PubMed Grisanti S, Guidry C (1995) Transdifferentiation of retinal pigment epithelial cells from epithelial to mesenchymal phenotype. Invest Ophthalmol Vis Sci 36: 391–405 PubMed
7.
Zurück zum Zitat Glaser BM, Cardin A, Biscoe B (1987) Proliferative vitreoretinopathy: The mechanism of development of vitreoretinal traction. Ophthalmology 94: 327–332 PubMed Glaser BM, Cardin A, Biscoe B (1987) Proliferative vitreoretinopathy: The mechanism of development of vitreoretinal traction. Ophthalmology 94: 327–332 PubMed
8.
Zurück zum Zitat Guidry C, McFarland R, Morris R et al. (1992) Collagen gel contraction by cells associated with proliferative vitreoretinopathy. Invest Ophthalmol Vis Sci 33: 2429–2435 PubMed Guidry C, McFarland R, Morris R et al. (1992) Collagen gel contraction by cells associated with proliferative vitreoretinopathy. Invest Ophthalmol Vis Sci 33: 2429–2435 PubMed
9.
Zurück zum Zitat Guidry C, Grinnel F (1985) Studies on the mechanism of hydrated collagen gel reorganization by human skin fibroblasts. J Cell Sci 79: 67–81 PubMed Guidry C, Grinnel F (1985) Studies on the mechanism of hydrated collagen gel reorganization by human skin fibroblasts. J Cell Sci 79: 67–81 PubMed
10.
Zurück zum Zitat Hardwick C, Feist R, Morris R et al. (1997) Tractional force generation by porcine müller cells: stimulation by growth factors in human vitreous. Invest Ophthalmol Vis Sci 38: 2053–2063 PubMed Hardwick C, Feist R, Morris R et al. (1997) Tractional force generation by porcine müller cells: stimulation by growth factors in human vitreous. Invest Ophthalmol Vis Sci 38: 2053–2063 PubMed
11.
Zurück zum Zitat Hardwick C, Morris R, Witherspoon D et al. (1995) Pathologic human vitreous promotes contraction by fibroblasts. Implications for proliferative vitreoretinopathy. Arch Ophthalmol 113: 1545–1553 PubMed Hardwick C, Morris R, Witherspoon D et al. (1995) Pathologic human vitreous promotes contraction by fibroblasts. Implications for proliferative vitreoretinopathy. Arch Ophthalmol 113: 1545–1553 PubMed
12.
Zurück zum Zitat Heidenkummer HP, Kampik A (1991) Immunohistochemical localization of epidermal growth factor receptor in a human epiretinal membrane. Graefes Arch Clin Exp Ophthalmol 229: 492–496 CrossRefPubMed Heidenkummer HP, Kampik A (1991) Immunohistochemical localization of epidermal growth factor receptor in a human epiretinal membrane. Graefes Arch Clin Exp Ophthalmol 229: 492–496 CrossRefPubMed
13.
Zurück zum Zitat Hunt R, Pakalnis V, Choudhury P, Black E (1992) Cytokines and serum cause a2b1 integrin-mediated contraction of collagen gels by cultured retinal pigment epithelial cells. Invest Ophthalmol Vis Sci 35: 955–963 Hunt R, Pakalnis V, Choudhury P, Black E (1992) Cytokines and serum cause a2b1 integrin-mediated contraction of collagen gels by cultured retinal pigment epithelial cells. Invest Ophthalmol Vis Sci 35: 955–963
14.
Zurück zum Zitat Jerdan JA, Pepose JS, Michels RG et al. (1989) Proliferative vitreoretinopathy membranes. An immunohistochemical study. Ophthalmology 96: 801–810 PubMed Jerdan JA, Pepose JS, Michels RG et al. (1989) Proliferative vitreoretinopathy membranes. An immunohistochemical study. Ophthalmology 96: 801–810 PubMed
15.
Zurück zum Zitat Kampik A, Kenyon K, Michels R et al. (2005) Epiretinal and vitreous membranes: comparative study of 56 cases. Arch Ophthalmol 25(Suppl 5): 1445–1454 Kampik A, Kenyon K, Michels R et al. (2005) Epiretinal and vitreous membranes: comparative study of 56 cases. Arch Ophthalmol 25(Suppl 5): 1445–1454
16.
Zurück zum Zitat Kupfer T, Ferguson T (1993) A potential pathophysiologic role for a2b1 integrin in human eye diseases involving vitreoretinal traction. FASEB 7: 1401 Kupfer T, Ferguson T (1993) A potential pathophysiologic role for a2b1 integrin in human eye diseases involving vitreoretinal traction. FASEB 7: 1401
17.
Zurück zum Zitat Laqua H, Machemer R (1975) Glial cell proliferation in retinal detachment (massive periretinal proliferation). Am J Ophthalmol 80: 602–618 PubMed Laqua H, Machemer R (1975) Glial cell proliferation in retinal detachment (massive periretinal proliferation). Am J Ophthalmol 80: 602–618 PubMed
18.
Zurück zum Zitat Laqua H, Machemer R (1977) Massive periretinale proliferation. I: Histologie. Ber Dtsch Ophthalmol Ges 74: 357–363 Laqua H, Machemer R (1977) Massive periretinale proliferation. I: Histologie. Ber Dtsch Ophthalmol Ges 74: 357–363
19.
Zurück zum Zitat Lashkari K, Rahimi N, Kazlauskas A (1999) Hepatocyte growth factor receptor in human RPE cells: implications in proliferative vitreoretinopathy. Invest Ophthalmol Vis Sci 40: 149–156 PubMed Lashkari K, Rahimi N, Kazlauskas A (1999) Hepatocyte growth factor receptor in human RPE cells: implications in proliferative vitreoretinopathy. Invest Ophthalmol Vis Sci 40: 149–156 PubMed
20.
Zurück zum Zitat Machemer R (1977) Massive periretinal proliferation: a logical approach to therapy. Trans Am Ophthalmol Soc 75: 556–586 PubMed Machemer R (1977) Massive periretinal proliferation: a logical approach to therapy. Trans Am Ophthalmol Soc 75: 556–586 PubMed
21.
Zurück zum Zitat Machemer R, Lacqua H (1975) Pigment epithelium proliferation in retinal detachment (massive periretinal proliferation). Am J Ophthalmol 80: 1–23 PubMed Machemer R, Lacqua H (1975) Pigment epithelium proliferation in retinal detachment (massive periretinal proliferation). Am J Ophthalmol 80: 1–23 PubMed
22.
Zurück zum Zitat Mandelcorn M, Machemer R, Fineberg E (1975) Proliferation and metaplasia of intravitreal retinal pigment epithelium cell autotransplant. Am J Ophthalmol 80: 227–237 PubMed Mandelcorn M, Machemer R, Fineberg E (1975) Proliferation and metaplasia of intravitreal retinal pigment epithelium cell autotransplant. Am J Ophthalmol 80: 227–237 PubMed
23.
Zurück zum Zitat Mazure A, Grierson I (1992) In vitro studies of the contractility of cell types involved in proliferative vitreoretinopathy. Invest Ophthlamol Vis Sci 33: 3407–3416 Mazure A, Grierson I (1992) In vitro studies of the contractility of cell types involved in proliferative vitreoretinopathy. Invest Ophthlamol Vis Sci 33: 3407–3416
24.
Zurück zum Zitat Mueller-Jensen K, Machemer R, Azarnia R (1975) Autotransplantation of retinal pigment epithelium in intravitreal diffusion chamber. Am J Ophthalmol 80: 530–537 PubMed Mueller-Jensen K, Machemer R, Azarnia R (1975) Autotransplantation of retinal pigment epithelium in intravitreal diffusion chamber. Am J Ophthalmol 80: 530–537 PubMed
25.
Zurück zum Zitat Newsome D, Rodrigues M, Machemer R (1981) Human massive periretinal proliferation. In vitro characteristics of cellular components. Arch Ophthalmol 99: 873–880 PubMed Newsome D, Rodrigues M, Machemer R (1981) Human massive periretinal proliferation. In vitro characteristics of cellular components. Arch Ophthalmol 99: 873–880 PubMed
26.
Zurück zum Zitat Pena RA, Jerdan JA, Glaser BM (1994) Effects of TGF-ß and TGF-ß neutralizing antibodies on fibroblast-induced collagen gel contraction: Implications for proliferative vitreoretinopathy. Invest Ophthalmol Vis Sci 35: 2804–2808 PubMed Pena RA, Jerdan JA, Glaser BM (1994) Effects of TGF-ß and TGF-ß neutralizing antibodies on fibroblast-induced collagen gel contraction: Implications for proliferative vitreoretinopathy. Invest Ophthalmol Vis Sci 35: 2804–2808 PubMed
27.
Zurück zum Zitat Radtke N, Tano Y, Chandler D, Machemer R (1981) Simulation of massive periretinal proliferation by autotransplantation of retinal pigment epithelial cells in rabbits. Am J Ophthalmol 91: 76–87 PubMed Radtke N, Tano Y, Chandler D, Machemer R (1981) Simulation of massive periretinal proliferation by autotransplantation of retinal pigment epithelial cells in rabbits. Am J Ophthalmol 91: 76–87 PubMed
28.
Zurück zum Zitat Raymond M, Thompson JT (1990) RPE-mediated collagen gel contraction. Inhibition by colchicine and stimulation by TGF-beta. Invest Ophthalmol Vis Sci 31: 1079–1086 PubMed Raymond M, Thompson JT (1990) RPE-mediated collagen gel contraction. Inhibition by colchicine and stimulation by TGF-beta. Invest Ophthalmol Vis Sci 31: 1079–1086 PubMed
29.
Zurück zum Zitat Sakamoto T, Hinton DR, Sakamoto H et al. (1994) Collagen gel contraction induced by retinal pigment epithelial cells and choroidal fibroblasts involves the protein kinase c pathway. Curr Eye Res 13: 451–459 CrossRefPubMed Sakamoto T, Hinton DR, Sakamoto H et al. (1994) Collagen gel contraction induced by retinal pigment epithelial cells and choroidal fibroblasts involves the protein kinase c pathway. Curr Eye Res 13: 451–459 CrossRefPubMed
30.
Zurück zum Zitat Schiro JA, Chan BM, Roswit WT et al. (1991) Integrin alpha 2 beta 1 (VLA-2) mediates reorganization and contraction of collagen matrices by human cells. Cell 67: 403–410 CrossRefPubMed Schiro JA, Chan BM, Roswit WT et al. (1991) Integrin alpha 2 beta 1 (VLA-2) mediates reorganization and contraction of collagen matrices by human cells. Cell 67: 403–410 CrossRefPubMed
31.
Zurück zum Zitat Vinores S, Campochiaro P, Conway B (1990) Ultrastructural and electronimmuno-cytocytochemical characterization of cells in epiretinal membranes. Invest Ophthalmol Vis Sci 31: 14–28 PubMed Vinores S, Campochiaro P, Conway B (1990) Ultrastructural and electronimmuno-cytocytochemical characterization of cells in epiretinal membranes. Invest Ophthalmol Vis Sci 31: 14–28 PubMed
32.
Zurück zum Zitat Webster L, Chignell AH, Limb GA (1999) Predominance of MMP-1 and MMP-2 in epiretinal and subretinal membranes of proliferative vitreoretinopathy. Exp Eye Res 68: 91–98 CrossRefPubMed Webster L, Chignell AH, Limb GA (1999) Predominance of MMP-1 and MMP-2 in epiretinal and subretinal membranes of proliferative vitreoretinopathy. Exp Eye Res 68: 91–98 CrossRefPubMed
33.
Zurück zum Zitat Wiedemann P (1992) Growth factors in retinal diseases: Proliferative vitroretinopathy, proliferative diabetic retinopathy and retinal degeneration. Surv Ophthalmol 36: 373–384 CrossRefPubMed Wiedemann P (1992) Growth factors in retinal diseases: Proliferative vitroretinopathy, proliferative diabetic retinopathy and retinal degeneration. Surv Ophthalmol 36: 373–384 CrossRefPubMed
Metadaten
Titel
Vitreal induzierte RPE-Zell-Traktion
Untersuchung pathologischer Glaskörperproben in einem In-vitro-Kontraktionsmodell
verfasst von
J. Beutel
M. Lüke
K.-U. Bartz-Schmidt
Prof. S. Grisanti
Publikationsdatum
01.10.2009
Verlag
Springer-Verlag
Erschienen in
Die Ophthalmologie / Ausgabe 10/2009
Print ISSN: 2731-720X
Elektronische ISSN: 2731-7218
DOI
https://doi.org/10.1007/s00347-008-1847-3