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Erschienen in: Comparative Clinical Pathology 5/2017

17.06.2017 | Original Article

Vitrification of bovine ovarian tissue: effect of perforated antral follicles on the structural preservation of follicles

verfasst von: A. Mogheiseh, F. Namazi, M. Kafi, S. Safarinejad, M. Azari, Y. Daneshbod

Erschienen in: Comparative Clinical Pathology | Ausgabe 5/2017

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Abstract

Despite all scientific studies, researches are continuing to increase outcomes of freezing ovarian tissue. The objective was to evaluate whether follicular perforation could improve the survival of antral follicles after vitrification. The present study was conducted on 20 bovine ovaries collected at slaughterhouse and a total of 344 antral follicles (less than 4 mm in diameter). For each ovary, three slices (approximately 1 × 1 cm) were separated from the cortex of each ovary and randomly were assigned in three groups, control (without freezing, fixed in 10% buffered formalin), non­perforated vitrified and perforated vitrified (after exposure to equilibration solution, antral follicles were immediately perforated) groups. After thawing, the tissue slices were evaluated histopathologically. Our histological evaluation showed that vitrification in group V1 did not significantly influence the degree of follicle damage compared to the follicles in fresh group as 69.8% of vitrified follicles were appeared as normal. Increasing follicular size had a significant effect on the vulnerability of follicles using different methods (p < 0.05). Therefore, perforation of follicles of bovine ovarian cortical tissue did not improve freezability of antral follicles by vitrification.
Literatur
Zurück zum Zitat Arav A, Zeron Y, Leslie SB, Behboodi E, Anderson GB, Crowe JH (1996) Phase transition temperature and chilling sensitivity of bovine oocytes cryobiology 33:589–599 Arav A, Zeron Y, Leslie SB, Behboodi E, Anderson GB, Crowe JH (1996) Phase transition temperature and chilling sensitivity of bovine oocytes cryobiology 33:589–599
Zurück zum Zitat Bordes A, Lornage J, Demirci B, Franck M, Courbiere B, Guerin JF, Salle B (2005) Normal gestations and live births after orthotopic autograft of vitrified–warmed hemi-ovaries into ewes. Hum Reprod, 20:2745–2748 Bordes A, Lornage J, Demirci B, Franck M, Courbiere B, Guerin JF, Salle B (2005) Normal gestations and live births after orthotopic autograft of vitrified–warmed hemi-ovaries into ewes. Hum Reprod, 20:2745–2748
Zurück zum Zitat Chen S-U, Lee T-H, Lien Y-R, Tsai Y-Y, Chang L-J, Yang Y-S (2005) Microsuction of blastocoelic fluid before vitrification increased survival and pregnancy of mouse expanded blastocysts, but pretreatment with the cytoskeletal stabilizer did not increase blastocyst survival. Fertil Steril 84:1156–1162CrossRefPubMed Chen S-U, Lee T-H, Lien Y-R, Tsai Y-Y, Chang L-J, Yang Y-S (2005) Microsuction of blastocoelic fluid before vitrification increased survival and pregnancy of mouse expanded blastocysts, but pretreatment with the cytoskeletal stabilizer did not increase blastocyst survival. Fertil Steril 84:1156–1162CrossRefPubMed
Zurück zum Zitat Chen S-U, Chien C-L, Wu M-Y, Chen T-H, Lai S-M, Lin C-W, Yang Y-S (2006) Novel direct cover vitrification for cryopreservation of ovarian tissues increases follicle viability and pregnancy capability in mice. Hum Reprod 21:2794–2800CrossRefPubMed Chen S-U, Chien C-L, Wu M-Y, Chen T-H, Lai S-M, Lin C-W, Yang Y-S (2006) Novel direct cover vitrification for cryopreservation of ovarian tissues increases follicle viability and pregnancy capability in mice. Hum Reprod 21:2794–2800CrossRefPubMed
Zurück zum Zitat Choi YH et al. (2011) Successful cryopreservation of expanded equine blastocysts. Theriogenology 76:143–152 Choi YH et al. (2011) Successful cryopreservation of expanded equine blastocysts. Theriogenology 76:143–152
Zurück zum Zitat Faheem M, Carvalhais I, Baron E, Moreira da Silva F (2013) Effect of bovine ovarian tissue vitrification on the structural preservation of antral follicles reproduction in domestic animals 48:774–780 Faheem M, Carvalhais I, Baron E, Moreira da Silva F (2013) Effect of bovine ovarian tissue vitrification on the structural preservation of antral follicles reproduction in domestic animals 48:774–780
Zurück zum Zitat Gandolfi F, Paffoni A, Brambilla EP, Bonetti S, Brevini TA, Ragni G (2006) Efficiency of equilibrium cooling and vitrification procedures for the cryopreservation of ovarian tissue: comparative analysis between human and animal models. Fertil Steril 85:1150–1156CrossRefPubMed Gandolfi F, Paffoni A, Brambilla EP, Bonetti S, Brevini TA, Ragni G (2006) Efficiency of equilibrium cooling and vitrification procedures for the cryopreservation of ovarian tissue: comparative analysis between human and animal models. Fertil Steril 85:1150–1156CrossRefPubMed
Zurück zum Zitat Gook DA, Edgar D, Borg J, Archer J, Mc Bain J (2005) Diagnostic assessment of the developmental potential of human cryopreserved ovarian tissue from multiple patients using xenografting. Hum Reprod 20:72–78CrossRefPubMed Gook DA, Edgar D, Borg J, Archer J, Mc Bain J (2005) Diagnostic assessment of the developmental potential of human cryopreserved ovarian tissue from multiple patients using xenografting. Hum Reprod 20:72–78CrossRefPubMed
Zurück zum Zitat Huang J, Tulandi T, Holzer H, Lau N, Macdonald S, Tan S, Chian R (2008) Cryopreservation of ovarian tissue and in vitro matured oocytes in a female with mosaic Turner syndrome: case report. Hum Reprod 23:336–339CrossRefPubMed Huang J, Tulandi T, Holzer H, Lau N, Macdonald S, Tan S, Chian R (2008) Cryopreservation of ovarian tissue and in vitro matured oocytes in a female with mosaic Turner syndrome: case report. Hum Reprod 23:336–339CrossRefPubMed
Zurück zum Zitat Imhof M, Hofstetter G, Bergmeister H, Rudas M, Kain R, Lipovac M, Huber J (2004) Cryopreservation of a whole ovary as a strategy for restoring ovarian function. J Assist Reprod Genet 21:459–465CrossRefPubMedPubMedCentral Imhof M, Hofstetter G, Bergmeister H, Rudas M, Kain R, Lipovac M, Huber J (2004) Cryopreservation of a whole ovary as a strategy for restoring ovarian function. J Assist Reprod Genet 21:459–465CrossRefPubMedPubMedCentral
Zurück zum Zitat Kagawa N, Silber S, Kuwayama M (2009) Successful vitrification of bovine and human ovarian tissue. Reprod BioMed Online 18:568–577CrossRefPubMed Kagawa N, Silber S, Kuwayama M (2009) Successful vitrification of bovine and human ovarian tissue. Reprod BioMed Online 18:568–577CrossRefPubMed
Zurück zum Zitat Kawamura K et al (2013) Hippo signaling disruption and Akt stimulation of ovarian follicles for infertility treatment. Proc Natl Acad Sci 110:17474–17479CrossRefPubMedPubMedCentral Kawamura K et al (2013) Hippo signaling disruption and Akt stimulation of ovarian follicles for infertility treatment. Proc Natl Acad Sci 110:17474–17479CrossRefPubMedPubMedCentral
Zurück zum Zitat Keros V et al (2009) Vitrification versus controlled-rate freezing in cryopreservation of human ovarian tissue. Hum Reprod 24:1670–1683CrossRefPubMed Keros V et al (2009) Vitrification versus controlled-rate freezing in cryopreservation of human ovarian tissue. Hum Reprod 24:1670–1683CrossRefPubMed
Zurück zum Zitat Lucci CM, Kacinskis MA, Lopes LHR, Rumpf R, Báo SN (2004) Effect of different cryoprotectants on the structural preservation of follicles in frozen zebu bovine (Bos indicus) ovarian tissue. Theriogenology 61:1101–1114CrossRefPubMed Lucci CM, Kacinskis MA, Lopes LHR, Rumpf R, Báo SN (2004) Effect of different cryoprotectants on the structural preservation of follicles in frozen zebu bovine (Bos indicus) ovarian tissue. Theriogenology 61:1101–1114CrossRefPubMed
Zurück zum Zitat Posillico S, Kader A, Falcone T, Agarwal A (2010) Ovarian tissue vitrification: modalities, challenges and potentials. Current Women's Health Reviews 6:352–366CrossRef Posillico S, Kader A, Falcone T, Agarwal A (2010) Ovarian tissue vitrification: modalities, challenges and potentials. Current Women's Health Reviews 6:352–366CrossRef
Zurück zum Zitat Rodrigues APR et al (2004) Cryopreservation of caprine ovarian tissue using dimethylsulphoxide and propanediol. Anim Reprod Sci 84:211–227CrossRefPubMed Rodrigues APR et al (2004) Cryopreservation of caprine ovarian tissue using dimethylsulphoxide and propanediol. Anim Reprod Sci 84:211–227CrossRefPubMed
Zurück zum Zitat Santos R, Tharasanit T, Van Haeften T, Figueiredo J, Silva J, Van den Hurk R (2007) Vitrification of goat preantral follicles enclosed in ovarian tissue by using conventional and solid-surface vitrification methods. Cell Tissue Res 327:167–176CrossRefPubMed Santos R, Tharasanit T, Van Haeften T, Figueiredo J, Silva J, Van den Hurk R (2007) Vitrification of goat preantral follicles enclosed in ovarian tissue by using conventional and solid-surface vitrification methods. Cell Tissue Res 327:167–176CrossRefPubMed
Zurück zum Zitat Tsuribe PM, CAM G, da Cruz Landim-Alvarenga F (2009) Viability of primordial follicles derived from cryopreserved ovine ovarian cortex tissue. Fertil Steril 91:1976–1983CrossRefPubMed Tsuribe PM, CAM G, da Cruz Landim-Alvarenga F (2009) Viability of primordial follicles derived from cryopreserved ovine ovarian cortex tissue. Fertil Steril 91:1976–1983CrossRefPubMed
Zurück zum Zitat Yavin S, Arav A (2007) Measurement of essential physical properties of vitrification solutions. Theriogenology 67:81–89CrossRefPubMed Yavin S, Arav A (2007) Measurement of essential physical properties of vitrification solutions. Theriogenology 67:81–89CrossRefPubMed
Zurück zum Zitat Youm HW, Lee JR, Lee J, Jee BC, Suh CS, Kim SH (2014) Optimal vitrification protocol for mouse ovarian tissue cryopreservation: effect of cryoprotective agents and in vitro culture on vitrified–warmed ovarian tissue survival. Hum Reprod 29:720–730CrossRefPubMed Youm HW, Lee JR, Lee J, Jee BC, Suh CS, Kim SH (2014) Optimal vitrification protocol for mouse ovarian tissue cryopreservation: effect of cryoprotective agents and in vitro culture on vitrified–warmed ovarian tissue survival. Hum Reprod 29:720–730CrossRefPubMed
Zurück zum Zitat Zeron Y, Ocheretny A, Kedar O, Borochov A, Sklan D, Arav A (2001) Seasonal changes in bovine fertility: relation to developmental competence of oocytes, membrane properties and fatty acid composition of follicles. Reproduction 121:447–454CrossRefPubMed Zeron Y, Ocheretny A, Kedar O, Borochov A, Sklan D, Arav A (2001) Seasonal changes in bovine fertility: relation to developmental competence of oocytes, membrane properties and fatty acid composition of follicles. Reproduction 121:447–454CrossRefPubMed
Metadaten
Titel
Vitrification of bovine ovarian tissue: effect of perforated antral follicles on the structural preservation of follicles
verfasst von
A. Mogheiseh
F. Namazi
M. Kafi
S. Safarinejad
M. Azari
Y. Daneshbod
Publikationsdatum
17.06.2017
Verlag
Springer London
Erschienen in
Comparative Clinical Pathology / Ausgabe 5/2017
Print ISSN: 1618-5641
Elektronische ISSN: 1618-565X
DOI
https://doi.org/10.1007/s00580-017-2506-3

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