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Journal of Assisted Reproduction and Genetics

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04.01.2020 | Technological Innovations

What human sperm RNA-Seq tells us about the microbiome

verfasst von: Grace M. Swanson, Sergey Moskovtsev, Clifford Librach, J. Richard Pilsner, Robert Goodrich, Stephen A. Krawetz

Erschienen in: Journal of Assisted Reproduction and Genetics | Ausgabe 2/2020

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Abstract

Purpose

The study was designed to assess the capacity of human sperm RNA-seq data to gauge the diversity of the associated microbiome within the ejaculate.

Methods

Semen samples were collected, and semen parameters evaluated at time of collection. Sperm RNA was isolated and subjected to RNA-seq. Microbial composition was determined by aligning sequencing reads not mapped to the human genome to the NCBI RefSeq bacterial, viral and archaeal genomes following RNA-Seq. Analysis of microbial assignments utilized phyloseq and vegan.

Results

Microbial composition within each sample was characterized as a function of microbial associated RNAs. Bacteria known to be associated with the male reproductive tract were present at similar levels in all samples representing 11 genera from four phyla with one exception, an outlier. Shannon diversity index (p < 0.001) and beta diversity (unweighted UniFrac distances, p = 9.99e-4; beta dispersion, p = 0.006) indicated the outlier was significantly different from all other samples. The outlier sample exhibited a dramatic increase in Streptococcus. Multiple testing indicated two operational taxonomic units, S. agalactiae and S. dysgalactiae (p = 0.009), were present.

Conclusion

These results provide a first look at the microbiome as a component of human sperm RNA sequencing that has sufficient sensitivity to identify contamination or potential pathogenic bacterial colonization at least among the known contributors.

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Titel: What human sperm RNA-Seq tells us about the microbiome
verfasst von: Grace M. Swanson
Sergey Moskovtsev
Clifford Librach
J. Richard Pilsner
Robert Goodrich
Stephen A. Krawetz
Publikationsdatum: 04.01.2020
Verlag: Springer US
Erschienen in: Journal of Assisted Reproduction and Genetics / Ausgabe 2/2020
Print ISSN: 1058-0468
Elektronische ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-019-01672-x

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What human sperm RNA-Seq tells us about the microbiome