Whole-genome sequence analysis of paris virus 1: a novel member of the genus Potyvirus infecting Paris polyphylla var. yunnanensis

The complete genome sequence of a novel potyvirus, tentatively named “paris virus 1” (ParV1, GenBank accession no. MN549985), infecting Paris polyphylla var. yunnanensis was determined in this study. A single large open reading frame (nt 96-9818) encoding a 3240-aa polyprotein that is predicted to be cleaved into 10 mature proteins was detected in the ParV1 genome. Comparative analysis of the ParV1 genome sequence with those of other potyviruses identified nine cleavage sites and conserved motifs that are typical features of potyviruses. Pairwise sequence comparisons showed that the ParV1 polyprotein shares 49.6-65.1% nucleotide and 47.1-68.9% amino acid sequence identity with viruses of the genus Potyvirus. BLAST analysis revealed that ParV1 shares 65.1% nucleotide and 68.9% amino acid sequence identity with Thunberg fritillary mosaic virus (TFMV, accession no. CAI59123), its closest known relative. These results suggest that paris virus 1 (ParV1) is a new member of the genus Potyvirus.