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Erschienen in: Diabetologia 6/2014

01.06.2014 | Article

Young men with low birthweight exhibit decreased plasticity of genome-wide muscle DNA methylation by high-fat overfeeding

verfasst von: Stine C. Jacobsen, Linn Gillberg, Jette Bork-Jensen, Rasmus Ribel-Madsen, Ester Lara, Vincenzo Calvanese, Charlotte Ling, Agustin F. Fernandez, Mario F. Fraga, Pernille Poulsen, Charlotte Brøns, Allan Vaag

Erschienen in: Diabetologia | Ausgabe 6/2014

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Abstract

Aims/hypothesis

The association between low birthweight (LBW) and risk of developing type 2 diabetes may involve epigenetic mechanisms, with skeletal muscle being a prime target tissue. Differential DNA methylation patterns have been observed in single genes in muscle tissue from type 2 diabetic and LBW individuals, and we recently showed multiple DNA methylation changes during short-term high-fat overfeeding in muscle of healthy people. In a randomised crossover study, we analysed genome-wide DNA promoter methylation in skeletal muscle of 17 young LBW men and 23 matched normal birthweight (NBW) men after a control and a 5 day high-fat overfeeding diet.

Methods

DNA methylation was measured using Illumina’s Infinium BeadArray covering 27,578 CpG sites representing 14,475 different genes.

Results

After correction for multiple comparisons, DNA methylation levels were found to be similar in the LBW and NBW groups during the control diet. Whereas widespread DNA methylation changes were observed in the NBW group in response to high-fat overfeeding, only a few methylation changes were seen in the LBW group (χ2, p < 0.001).

Conclusions/interpretation

Our results indicate lower DNA methylation plasticity in skeletal muscle from LBW vs NBW men, potentially contributing to understanding the link between LBW and increased risk of type 2 diabetes.
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Literatur
1.
Zurück zum Zitat Harder T, Rodekamp E, Schellong K, Dudenhausen JW, Plagemann A (2007) Birth weight and subsequent risk of type 2 diabetes: a meta-analysis. Am J Epidemiol 165:849–857PubMedCrossRef Harder T, Rodekamp E, Schellong K, Dudenhausen JW, Plagemann A (2007) Birth weight and subsequent risk of type 2 diabetes: a meta-analysis. Am J Epidemiol 165:849–857PubMedCrossRef
2.
Zurück zum Zitat Barres R, Osler ME, Yan J et al (2009) Non-CpG methylation of the PGC-1alpha promoter through DNMT3B controls mitochondrial density. Cell Metab 10:189–198PubMedCrossRef Barres R, Osler ME, Yan J et al (2009) Non-CpG methylation of the PGC-1alpha promoter through DNMT3B controls mitochondrial density. Cell Metab 10:189–198PubMedCrossRef
3.
Zurück zum Zitat Kirchner H, Osler ME, Krook A, Zierath JR (2013) Epigenetic flexibility in metabolic regulation: disease cause and prevention? Trends Cell Biol 23:203–209PubMedCrossRef Kirchner H, Osler ME, Krook A, Zierath JR (2013) Epigenetic flexibility in metabolic regulation: disease cause and prevention? Trends Cell Biol 23:203–209PubMedCrossRef
4.
Zurück zum Zitat Ribel-Madsen R, Fraga MF, Jacobsen S et al (2012) Genome-wide analysis of DNA methylation differences in muscle and fat from monozygotic twins discordant for type 2 diabetes. PLoS ONE 7:e51302PubMedCentralPubMedCrossRef Ribel-Madsen R, Fraga MF, Jacobsen S et al (2012) Genome-wide analysis of DNA methylation differences in muscle and fat from monozygotic twins discordant for type 2 diabetes. PLoS ONE 7:e51302PubMedCentralPubMedCrossRef
5.
Zurück zum Zitat Tobi EW, Lumey LH, Talens RP et al (2009) DNA methylation differences after exposure to prenatal famine are common and timing- and sex-specific. Hum Mol Genet 18:4046–4053PubMedCentralPubMedCrossRef Tobi EW, Lumey LH, Talens RP et al (2009) DNA methylation differences after exposure to prenatal famine are common and timing- and sex-specific. Hum Mol Genet 18:4046–4053PubMedCentralPubMedCrossRef
6.
Zurück zum Zitat Brøns C, Jacobsen S, Nilsson E et al (2010) Deoxyribonucleic acid methylation and gene expression of PPARGC1A in human muscle is influenced by high-fat overfeeding in a birth-weight-dependent manner. J Clin Endocrinol Metab 95:3048–3056 Brøns C, Jacobsen S, Nilsson E et al (2010) Deoxyribonucleic acid methylation and gene expression of PPARGC1A in human muscle is influenced by high-fat overfeeding in a birth-weight-dependent manner. J Clin Endocrinol Metab 95:3048–3056
7.
Zurück zum Zitat Jacobsen SC, Brøns C, Bork-Jensen J et al (2012) Effects of short-term high-fat overfeeding on genome-wide DNA methylation in the skeletal muscle of healthy young men. Diabetologia 55:3341–3349 Jacobsen SC, Brøns C, Bork-Jensen J et al (2012) Effects of short-term high-fat overfeeding on genome-wide DNA methylation in the skeletal muscle of healthy young men. Diabetologia 55:3341–3349
8.
Zurück zum Zitat Brøns C, Jacobsen S, Hiscock N et al (2012) Effects of high-fat overfeeding on mitochondrial function, glucose and fat metabolism, and adipokine levels in low-birth-weight subjects. Am J Physiol Endocrinol Metab 302:E43–E51 Brøns C, Jacobsen S, Hiscock N et al (2012) Effects of high-fat overfeeding on mitochondrial function, glucose and fat metabolism, and adipokine levels in low-birth-weight subjects. Am J Physiol Endocrinol Metab 302:E43–E51
9.
Zurück zum Zitat Doria A, Patti ME, Kahn CR (2008) The emerging genetic architecture of type 2 diabetes. Cell Metab 8:186–200PubMedCrossRef Doria A, Patti ME, Kahn CR (2008) The emerging genetic architecture of type 2 diabetes. Cell Metab 8:186–200PubMedCrossRef
10.
Zurück zum Zitat Barres R, Kirchner H, Rasmussen M et al (2013) Weight loss after gastric bypass surgery in human obesity remodels promoter methylation. Cell Rep 3:1020–1027PubMedCrossRef Barres R, Kirchner H, Rasmussen M et al (2013) Weight loss after gastric bypass surgery in human obesity remodels promoter methylation. Cell Rep 3:1020–1027PubMedCrossRef
Metadaten
Titel
Young men with low birthweight exhibit decreased plasticity of genome-wide muscle DNA methylation by high-fat overfeeding
verfasst von
Stine C. Jacobsen
Linn Gillberg
Jette Bork-Jensen
Rasmus Ribel-Madsen
Ester Lara
Vincenzo Calvanese
Charlotte Ling
Agustin F. Fernandez
Mario F. Fraga
Pernille Poulsen
Charlotte Brøns
Allan Vaag
Publikationsdatum
01.06.2014
Verlag
Springer Berlin Heidelberg
Erschienen in
Diabetologia / Ausgabe 6/2014
Print ISSN: 0012-186X
Elektronische ISSN: 1432-0428
DOI
https://doi.org/10.1007/s00125-014-3198-8

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