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01.12.2018 | Research | Ausgabe 1/2018 Open Access

Virology Journal 1/2018

Zika virus-induced hyper excitation precedes death of mouse primary neuron

Zeitschrift:
Virology Journal > Ausgabe 1/2018
Autoren:
Julie Gaburro, Asim Bhatti, Vinod Sundaramoorthy, Megan Dearnley, Diane Green, Saeid Nahavandi, Prasad N. Paradkar, Jean-Bernard Duchemin
Wichtige Hinweise

Electronic supplementary material

The online version of this article (https://​doi.​org/​10.​1186/​s12985-018-0989-4) contains supplementary material, which is available to authorized users.

Abstract

Background

Zika virus infection in new born is linked to congenital syndromes, especially microcephaly. Studies have shown that these neuropathies are the result of significant death of neuronal progenitor cells in the central nervous system of the embryo, targeted by the virus. Although cell death via apoptosis is well acknowledged, little is known about possible pathogenic cellular mechanisms triggering cell death in neurons.

Methods

We used in vitro embryonic mouse primary neuron cultures to study possible upstream cellular mechanisms of cell death. Neuronal networks were grown on microelectrode array and electrical activity was recorded at different times post Zika virus infection. In addition to this method, we used confocal microscopy and Q-PCR techniques to observe morphological and molecular changes after infection.

Results

Zika virus infection of mouse primary neurons triggers an early spiking excitation of neuron cultures, followed by dramatic loss of this activity. Using NMDA receptor antagonist, we show that this excitotoxicity mechanism, likely via glutamate, could also contribute to the observed nervous system defects in human embryos and could open new perspective regarding the causes of adult neuropathies.

Conclusions

This model of excitotoxicity, in the context of neurotropic virus infection, highlights the significance of neuronal activity recording with microelectrode array and possibility of more than one lethal mechanism after Zika virus infection in the nervous system.
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