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Erschienen in: Inflammation 3/2013

01.06.2013

Zinc Sulfate Inhibited Inflammation of Der p2-Induced Airway Smooth Muscle Cells by Suppressing ERK1/2 and NF-κB Phosphorylation

verfasst von: Chia-Ju Shih, Ya-Ling Chiou

Erschienen in: Inflammation | Ausgabe 3/2013

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Abstract

Inflammation of airway smooth muscle cells (ASMCs) is believed to be important in causing airway hyperresponsiveness. However, zinc has been reported to be implicated in many kinds of cell inflammation. Little is known about the effect of zinc treatment on Der p2 (group II Dermatophagoides pteronyssinus)-induced inflammation from ASMCs. This study investigated effects and mechanisms of zinc in Der p2-treated ASMCs. Der p2-treated primary ASMCs were cultured with various concentrations of zinc sulfate (ZnSO4) 6 μM, 12 μM, 24 μM, and 96 μM. The proteins and mRNAs of cytokines in ASMCs were examined by ELISA and real-time PCR. Intracellular zinc was stained with Zinquin fluorescence. The cell signaling protein expression was detected by Western blot. Der p2 was used to induce interleukin (IL)-6, IL-8, IL-1, and monocyte chemotactic protein-1 production of ASMCs. However, we found that 24 μM ZnSO4 reduced these inflammatory mediators production of Der p2-treated primary ASMCs. Der p2-induced extracellular signal-regulated kinases (ERK) and nuclear factor-kappa B (NF-κB) phosphorylation were suppressed by supplementation of 24 μM ZnSO4. Zinc is an anti-inflammatory agent that reduces inflammation of Der p2-treated ASMCs through the suppression of the ERK and NF-κB pathway. The results may be helpful for the development of effective treatments.
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Metadaten
Titel
Zinc Sulfate Inhibited Inflammation of Der p2-Induced Airway Smooth Muscle Cells by Suppressing ERK1/2 and NF-κB Phosphorylation
verfasst von
Chia-Ju Shih
Ya-Ling Chiou
Publikationsdatum
01.06.2013
Verlag
Springer US
Erschienen in
Inflammation / Ausgabe 3/2013
Print ISSN: 0360-3997
Elektronische ISSN: 1573-2576
DOI
https://doi.org/10.1007/s10753-012-9583-x

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