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01.12.2014 | Research article | Ausgabe 1/2014 Open Access

BMC Gastroenterology 1/2014

Zinc treatment ameliorates diarrhea and intestinal inflammation in undernourished rats

Zeitschrift:
BMC Gastroenterology > Ausgabe 1/2014
Autoren:
Camila AA de Queiroz, Said Gonçalves C Fonseca, Priscila B Frota, Ítalo L Figueiredo, Karoline S Aragão, Carlos Emanuel C Magalhães, Cibele BM de Carvalho, Aldo Ângelo M Lima, Ronaldo A Ribeiro, Richard L Guerrant, Sean R Moore, Reinaldo B Oriá
Wichtige Hinweise

Electronic supplementary material

The online version of this article (doi:10.​1186/​1471-230X-14-136) contains supplementary material, which is available to authorized users.

Competing interest

All authors declare no competing of interest.

Authors’ contributions

CCAQ, RLG, AAML, SRM, and RBO contributed with the study design, data analyses, and manuscript preparation. CCAQ, ILF, and PBF conducted animal handling and molecular biology analyses. SGCF worked in the diet manipulation and production. CCAQ and CBMC were responsible for the bacterial translocation experiments. CECM, RAR, and KSA conducted the serum zinc measurements and ELISA studies. All authors read and approved the final manuscript.

Abstract

Background

WHO guidelines recommend zinc supplementation as a key adjunct therapy for childhood diarrhea in developing countries, however zinc’s anti-diarrheal effects remain only partially understood. Recently, it has been recognized that low-grade inflammation may influence stunting. In this study, we examined whether oral zinc supplementation could improve weight, intestinal inflammation, and diarrhea in undernourished weanling rats.

Methods

Rats were undernourished using a northeastern Brazil regional diet (RBD) for two weeks, followed by oral gavage with a saturated lactose solution (30 g/kg) in the last 7 days to induce osmotic diarrhea. Animals were checked for diarrhea daily after lactose intake. Blood was drawn in order to measure serum zinc levels by atomic absorption spectroscopy. Rats were euthanized to harvest jejunal tissue for histology and cytokine profiles by ELISA. In a subset of animals, spleen samples were harvested under aseptic conditions to quantify bacterial translocation.

Results

Oral zinc supplementation increased serum zinc levels following lactose-induced osmotic diarrhea. In undernourished rats, zinc improved weight gain following osmotic diarrhea and significantly reduced diarrheal scores by the third day of lactose intake (p < 0.05), with improved jejunum histology (p < 0.0001). Zinc supplementation diminished bacterial translocation only in lactose-challenged undernourished rats (p = 0.03) compared with the untreated challenged controls and reduced intestinal IL-1β and TNF-α cytokines to control levels.

Conclusion

Altogether our findings provide novel mechanisms of zinc action in the setting of diarrhea and undernutrition and support the use of zinc to prevent the vicious cycle of malnutrition and diarrhea.
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