Altered dendritic cell distribution in patients with common variable immunodeficiency

Common variable immunodeficiency (CVID) is a primary immunodeficiency disease characterized by a low concentration of serum immunoglobulins and an impaired antibody response to challenging antigens [1]. Although the pathophysiology of CVID is heterogeneous and largely unknown, several causes leading to an alteration of immunoglobulin concentrations in the blood have already been identified. These include a failure of B-cell maturation, including altered somatic hypermutation [2]; defective cell-membrane signaling [3]; T-cell abnormalities such as reduced expression of key membrane-expressed molecules (CD40 ligand, ICOS (inducible costimulator protein), L-selectin) [4]; impaired cytokine production [5]; and a reduced generation of antigen-specific memory T cells [6].

Whereas the antigen-presenting function of DCs has been reported to be normal in CVID [7, 8], their involvement in the origin of some CVID cannot be ruled out, as these cells are known to interact directly with B cells, to present antigen to T cells, and to produce cytokines implicated in B-cell differentiation [9]. Two major pathways of differentiation generating DCs are thought to exist, according to their membrane expression of the β-integrin CD11c [10]. Myeloid DCs (mDCs) include skin Langerhans' cells and interstitial DCs and express CD11c at their surface. In contrast, plasmacytoid DCs (pDCs), which do not express CD11c, are CD123⁺. Recent data suggest a role for DCs in B-cell growth and differentiation, as the release by mDCs of soluble factors such as IL-12 and IL-6 and/or membrane molecules such as BAFF/APRIL induces the activation and the differentiation of normal B cells [11, 12]. In addition, the observation that pDCs directly induce the differentiation of plasma cells into immunoglobulin-secreting plasma cells suggests that pDCs are critically involved in humoral responses [13, 14]. Altogether, these new data prompted us to examine the blood distribution of DC subsets in 44 patients with CVID.

The median absolute count of circulating pDCs was significantly lower in CVID patients than in controls (P = 0.002). Although CVID patients had a lower mean mDC count, their median absolute count was not statistically significantly different from that of controls (P = 0.1) (Fig. 1).

However, when CVID patients were segregated in accordance with the Warnatz classification, the DC subset distributions were quite different between groups (Table 1). Groups 1a and 3 had statistically significantly lower levels of circulating DCs of both types than controls, but this difference was far more dramatic concerning the pDC subset (P = 0.001 and P = 0.01 for mDCs and P = 0.00005 and 0.0003 for pDCs, respectively). In groups 1b and 2, blood pDCs and mDCs were not statistically different in comparison with those in controls.

When the various groups of CVID patients were compared with each other, the median absolute count of circulating mDCs was found to be significantly reduced in groups 1a and 3 vs group 2 (P = 0.009 and P = 0.02, respectively). Although the median values of mDCs were lower in group 1a than in group 1b, the difference did not reach statistical significance (P = 0.1). In contrast, CVID patients in groups 1a and 3 had significantly lower pDCs median counts than those in group 2 (P = 0.0005 and P = 0.001, respectively), and, to a lesser extent, in group 1b (P = 0.02 and P = 0.009). No differences were observed between group 1a and group 3.

Moreover, we found a significant inverse statistical correlation between the low peripheral pDC levels of 19 CVID patients and the expression of the chemokine receptor CCR7 (R = -2.31, P = 0.03, Spearman test). These 19 patients were followed in the Department of Internal Medicine at Haut-Lévêque Hospital, directed by JL Pellegrin and JF Viallard. They were chosen because blood samples could easily be taken, in consideration of the short time-period available to perform new experiments with chemokine receptors. Of these 19 patients, 5 belonged to group Ia, 5 to group Ib, 6 to group II and 3 to group III. No differences were observed between the different groups with regard to the expression of other chemokine receptors including CCR2, CCR5, CXCR3, and CXCR4. This observation is in agreement with the already published data concerning the CCL19- and CCL21-dependent migration of mature pDCs to lymph nodes [17].

The most salient feature of the present study concerns patients in group 1a, who were at higher risk of autoimmune diseases (10 of 13 patients) and splenomegaly (9 of 13), and in group 3, both groups exhibiting a marked decrease in their peripheral pDC levels. Patients in group 2, who did not develop such complications, were similar to healthy individuals.

From these preliminary observations we can only speculate on the diminished pDC counts observed in the patients of groups 1a and 3. The migratory properties of DCs seem to be altered, leading to sequestration in tissue and/or secondary lymphoid organs due to an alteration of chemokine receptor membrane expression and maturation. However, we cannot rule out a defect in their function, such as has been suggested regarding DCs derived in vitro from monocytes [18].

At least in some patients, hypogammaglobulinemia could rely on an alteration of the innate immunity, since pDCs seem to play a critical role in the generation of antibody responses: they have been shown in vitro to control the differentiation of activated B cells into plasma cells through the secretion of interferon α and β and of IL-6 [13]. B cells activated with pDCs preferentially secrete IgG over IgM, suggesting that pDCs may specifically target memory B cells, whereas mDCs, which can enhance B-cell proliferation and isotype switching toward IgA [19], as well as plasma cell differentiation [20], could induce the differentiation of naive B cells through the secretion of IL-12 and IL-6 [11]. Granulocyte-colony-stimulating factor and FLT3 ligand, through their ability to mobilize dendritic cells, may be a new and valuable therapeutic alternative for CVID patients.

Our data provide evidence of a profound alteration in the distribution of subsets of DCs, especially pDCs, in the peripheral blood of CVID patients. Taken together, our results raise the possibility that innate immunity is involved in CVID pathogenesis.