nach oben

Journal of Infection and Chemotherapy

Erschienen in:

01.06.2012 | Original Article

Apoptosis of bladder cancer by sodium butyrate and cisplatin

verfasst von: Takuo Maruyama, Shingo Yamamoto, Jun Qiu, Yasuo Ueda, Toru Suzuki, Michio Nojima, Hiroki Shima

Erschienen in: Journal of Infection and Chemotherapy | Ausgabe 3/2012

Einloggen, um Zugang zu erhalten

Abstract

The effects of sodium butyrate (SB), a histone deacetylase inhibitor, in combination with cisplatin (CDDP), for inhibition of cell growth and induction of apoptosis were investigated in bladder cancer cell lines in vitro. Bladder cancer cell lines T24, 253J, and UMUC3 were treated with different concentrations of CDDP or SB. Cell proliferation was studied by XTT assay. Cell-cycle analysis and induction of apoptosis were analyzed by laser scanning cytometry (LSC). Western blot analysis was used to determine expression of p21, p27, TRADD, FADD, caspase-2, and caspase-7. We observed that SB in combination with CDDP induced significant inhibition of cell growth in a dose-dependent manner through G₁ arrest and apoptosis, as determined by LSC. When bladder cancer cell lines were treated with SB plus CDDP, Western blotting showed increased expression of p21 but not p27 in T24 cells, whereas both p21 and p27 increased in 253J and UMUC3 cells. All cell lines exhibited a moderate increase in TRADD and decrease in procaspase-2 but no significant change in FADD and procaspase-7. The results showed the synergistic anticancer effect of SB in combination with CDDP, their potential for treatment of bladder cancer, and their mechanism of action in terms of cell signal transduction and induction of apoptosis.

Jemal A, Siegel R, Ward E, Hao Y, Xu J, Thun MJ. Cancer statistics, 2009. CA Cancer J Clin. 2009;59:225–49.PubMedCrossRef

Sawa H, Murakami H, Ohshima Y, Sugino T, Nakajyo T, Kisanuki T, et al. Histone deacetylase inhibitors such as sodium butyrate and trichostatin A induce apoptosis through an increase of the bcl-2-related protein Bad. Brain Tumor Pathol. 2001;18:109–14.PubMedCrossRef

Hernandez A, Thomas R, Smith F, Sandberg J, Kim S, Chung DH, et al. Butyrate sensitizes human colon cancer cells to TRAIL-mediated apoptosis. Surgery (St. Louis). 2001;130:265–72.CrossRef

Chopin V, Toillon RA, Jouy N, Le Bourhis X. Sodium butyrate induces P53-independent, Fas-mediated apoptosis in MCF-7 human breast cancer cells. Br J Pharmacol. 2002;135:79–86.PubMedCrossRef

Marks P, Rifkind RA, Richon VM, Breslow R, Miller T, Kelly WK. Histone deacetylases and cancer: causes and therapies. Nat Rev Cancer. 2001;1:194–202.PubMedCrossRef

Marks PA, Richon VM, Breslow R, Rifkind RA. Histone deacetylases inhibitors as new cancer drugs. Curr Opin Oncol. 2001;13:477–83.PubMedCrossRef

Vigushin DM, Coombes RC. Histone deacetylases inhibitors in cancer treatment. Anticancer Drugs. 2002;13:1–13.PubMedCrossRef

Gray SG, Ekström TJ. The human histone deacetylase family. Exp Cell Res. 2001;262:75–83.PubMedCrossRef

Yoshida M, Horinouchi S, Beppu T. Trichostatin A and trapoxin: novel chemical probes for the role of histone acetylation in chromatin structure and function. Bioessays. 1995;17:423–30.PubMedCrossRef

10.

Strait KA, Dabbas B, Hammond EH, Warnick CT, Iistrup SJ, Ford CD. Cell cycle blockade and differentiation of ovarian cancer cells by the histone deacetylase inhibitor trichostatin A are associated with changes in p21, Rb, and Id proteins. Mol Cancer Ther. 2002;1:1181–90.PubMed

11.

Donadelli M, Costanzo C, Faggioli L, Scupoli MT, Moore PS, Bassi C, et al. Trichostatin A, an inhibitor of histone deacetylases, strongly suppresses growth of pancreatic adenocarcinoma cells. Mol Carcinog. 2003;38:59–69.PubMedCrossRef

12.

Chopin V, Toillon RA, Jouy N, Le Bourhis X. P21(WAF1/CIP1) is dispensable for G₁ arrest, but indispensable for apoptosis induced by sodium butyrate in MCF-7 breast cancer cells. Oncogene. 2004;23:21–9.PubMedCrossRef

13.

Wang ZM, Hu J, Zhou D, Xu ZY, Panasci LC, Chen ZP. Trichostatin A inhibits proliferation and induces expression of P21WAF and p27 in human brain tumor cell lines. Ai Zheng. 2002;21:1100–5.PubMed

14.

Chen Z, Clark S, Birkeland M, Sung CM, Lago A, Liu R, et al. Induction and super-induction of growth arrest and DNA damage 45(GADD45) alpha and beta messenger RNAs by histone deacetylase inhibitors trichostatin A (TSA) and butyrate in SW620 human colon carcinoma cells. Cancer Lett. 2002;188:127–40.PubMedCrossRef

15.

Kim YB, Ki SW, Yoshida M, Horinouchi S. Mechanism of cell cycle arrest caused by histone deacetylase inhibitors in human carcinoma cells. J Antibiot (Tokyo). 2000;53:1191–200.CrossRef

16.

Li GC, Zhang X, Pan TJ, Chen Z, Ye ZQ. Histone deacetylase inhibitor trichostatin A inhibits the growth of bladder cancer cells through induction of p21WAF1 and G₁ cell cycle arrest. Int J Urol. 2006;13:581–6.PubMedCrossRef

17.

Gould JJ, Kenney PA, Rieger-Christ KM, Silva Neto B, Wszolek MF, LaVoie A, et al. Identification of tumor and invasion suppressor gene modulators in bladder cancer by different classes of histone deacetylase inhibitors using reverse phase protein arrays. J Urol. 2010;183:2395–402.PubMedCrossRef

18.

Wang D, Wang Z, Tian B, Li X, Li S, Tian Y. Two hour exposure to sodium butyrate sensitizes bladder cancer to anticancer drugs. Int J Urol. 2008;15:435–41.PubMedCrossRef

19.

Hara I, Miyake H, Hara S, Arakawa S, Kamidono S. Sodium butyrate induces apoptosis in human renal cell carcinoma cells and synergistically enhances their sensitivity to anti-Fas-mediated cytotoxicity. Int J Oncol. 2000;17:1213–8.PubMed

20.

Timmermann S, Lehrmann H, Polesskaya A, Harel-Bellan A. Histone acetylation and disease. Cell Mol Life Sci. 2001;58:728–36.PubMedCrossRef

21.

Qiu J, Yamamoto S, Maruyama T, Kondoh N, Nojima M, Shima H. Growth suppression of prostate cancer cells by sodium butyrate in vitro. Acta Med Hyogo. 2007;32:77–85.

22.

Xiong Y, Hannon GJ, Zhang H, Casso D, Kobayashi R, Beach D. p21 is a universal inhibitor of cyclin kinase. Nature (Lond). 1993;366:701–4.CrossRef

23.

Harper JW, Adami GR, Wei N, Keyomarsi K, Elledge SJ. The p21 cdk-interacting protein Cipl is a potent inhibitor of G₁ cyclin-dependent kinases. Cell. 1993;75:805–16.PubMedCrossRef

24.

el-Deiry WS, Tokino T, Velculescu VE, Levy DB, Parsons R, Trent JM, et al. WAF1, a potential mediator of p53 tumor suppression. Cell. 1993;75:817–25.PubMedCrossRef

25.

Lacombe L, Orlow I, Silver D, Gerald WL, Fair WR, Reuter VE, et al. Analysis of p21Waf1/CIP1 in primary bladder tumors. Oncol Res. 1996;8:409–14.PubMed

26.

Richon VM, Sandhoff TW, Rifkind RA, Marks PA. Histone deacetylase inhibitor selectively induces p21WAF1 expression and gene-associated histone acetylation. Proc Natl Acad Sci USA. 2000;97:10014–9.PubMedCrossRef

27.

Hashimoto-Tamaoki T. Control of the cell cycle: cloning and functional analyses of the tumor suppressor genes. Acta Med Hyogo. 2002;27:167–81.

28.

Shang D, Liu Y, Matsui Y, Ito N, Nishiyama H, Kamoto T, et al. Demethylating agent 5-aza-2′-deoxycytidine enhances susceptibility of bladder transitional cell carcinoma to cisplatin. Urology. 2008;71:1220–5.PubMedCrossRef

29.

Rokhlin OW, Bishop GA, Hostager BS, Waldschmidt TJ, Sidorenko SP, Pavloff N, et al. Fas-mediated apoptosis in human prostatic carcinoma cell lines. Cancer Res. 1997;57:1758–68.PubMed

30.

Los M, Wesselborg S, Schulze-Osthoff K. The role of caspases in development, immunity, and apoptotic signal transduction: lessons from knockout mice. Immunity. 1999;10:629–39.PubMedCrossRef

31.

McKnight JJ, Gray SB, O’Kane HF, Johnston SR, Williamson KE. Apoptosis and chemotherapy for bladder cancer. J Urol. 2005;173:683–90.PubMedCrossRef

32.

Wilson NS, Dixit V, Ashkenazi A. Death receptor signal transducers: nodes of coordination in immune signaling networks. Nat Immunol. 2009;10:348–55.PubMedCrossRef

33.

Kinoshita H, Yoshikawa H, Shiiki K, Hamada Y, Nakajima Y, Tasaka K. Cisplatin (CDDP) sensitizes human osteosarcoma cell to Fas/CD95-mediated apoptosis by down-regulating FLIP-L expression. Int J Cancer. 2000;88:986–91.PubMedCrossRef

34.

Ho LH, Taylor R, Dorstyn L, Cakouros D, Bouillet P, Kumar S. A tumor suppressor function for caspase-2. Proc Natl Acad Sci USA. 2009;106:5336–41.PubMedCrossRef

35.

Yano T, Itoh Y, Matsuo M, Kawashiri T, Egashira N, Oishi R. Involvement of both tumor necrosis factor-alpha-induced necrosis and p53-mediated caspase-dependent apoptosis in nephrotoxicity of cisplatin. Apoptosis. 2007;12:1901–9.PubMedCrossRef

36.

Lin J, Zhang Z, Yang J, Zeng S, Liu BF, Luo Q. Real-time detection of caspase-2 activation in a single living HeLa cell during cisplatin-induced apoptosis. J Biomed Opt. 2006;11:024011.PubMedCrossRef

Titel: Apoptosis of bladder cancer by sodium butyrate and cisplatin
verfasst von: Takuo Maruyama
Shingo Yamamoto
Jun Qiu
Yasuo Ueda
Toru Suzuki
Michio Nojima
Hiroki Shima
Publikationsdatum: 01.06.2012
Verlag: Springer Japan
Erschienen in: Journal of Infection and Chemotherapy / Ausgabe 3/2012
Print ISSN: 1341-321X
Elektronische ISSN: 1437-7780
DOI: https://doi.org/10.1007/s10156-011-0322-2

Leitlinien kompakt für die Innere Medizin

Mit medbee Pocketcards sicher entscheiden.

^{Seit 2022 gehört die medbee GmbH zum Springer Medizin Verlag}

Kostenlos registrieren

Update Innere Medizin

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert.

Newsletter bestellen

Live-Webinar "Urologie und Sexualmedizin in der Praxis"

Springer Medizin

Apoptosis of bladder cancer by sodium butyrate and cisplatin

Abstract

Leitlinien kompakt für die Innere Medizin

Neu im Fachgebiet Innere Medizin

Erhebliches Risiko für Kehlkopfkrebs bei mäßiger Dysplasie

Nach Herzinfarkt mit Typ-1-Diabetes schlechtere Karten als mit Typ 2?

15% bedauern gewählte Blasenkrebs-Therapie

Costims – das nächste heiße Ding in der Krebstherapie?

Update Innere Medizin

Live-Webinar "Urologie und Sexualmedizin in der Praxis"

Springer Medizin

Abstract

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 3/2012

The validity of the criteria for primary infection of Chlamydophila pneumoniae in children by measuring ELISA IgM antibodies

Performance evaluation of detecting adenovirus by using rapid diagnostic kits among Japanese people

Vigorous cleaning and adequate ventilation are necessary to control an outbreak in a neonatal intensive care unit

Clinical characteristics of healthcare-associated pneumonia in a public hospital in a metropolitan area of Japan

A case study of measles vaccination for university students during the measles outbreak in Tokyo, Japan, 2007

Should urologists care for the pharyngeal infection of Neisseria gonorrhoeae or Chlamydia trachomatis when we treat male urethritis?

Leitlinien kompakt für die Innere Medizin

Neu im Fachgebiet Innere Medizin

Erhebliches Risiko für Kehlkopfkrebs bei mäßiger Dysplasie

Nach Herzinfarkt mit Typ-1-Diabetes schlechtere Karten als mit Typ 2?

15% bedauern gewählte Blasenkrebs-Therapie

Costims – das nächste heiße Ding in der Krebstherapie?

Update Innere Medizin