nach oben

Erschienen in:

01.06.2013 | Interventional Neuroradiology

Autologous mesenchymal stem cell endografting in experimental cerebrovascular aneurysms

verfasst von: Aymeric Rouchaud, Clément Journé, Liliane Louedec, Véronique Ollivier, Mohammed Derkaoui, Jean-Baptiste Michel, Mikael Mazighi

Erschienen in: Neuroradiology | Ausgabe 6/2013

Einloggen, um Zugang zu erhalten

Abstract

Introduction

Coiling is the gold standard for the treatment of intracranial aneurysms. However, some issues associated with endovascular treatment limit its long-term efficiency. Recanalization with coil compaction is certainly the most important. New approaches may be considered to promote thrombus colonization by mesenchymal cells and aneurysm healing. In the present study, we have percutaneously delivered autologous bone marrow mesenchymal stem cells (BMSCs) to an elastase-induced rabbit carotid aneurysm model in vivo.

Methods

Autologous mesenchymatous stem cells were obtained after femoral puncture and bone marrow aspiration. After 2 weeks of in vitro cell culture, five million BMSCs were grafted in the carotid aneurysm using an endovascular approach.

Results

We demonstrated the feasibility of in vivo percutaneous seeding of autologous BMSCs in the aneurysm by positive Hoechst fluorostaining. Two weeks later, conventional angiography showed an increase in median aneurysmal surface in the sham group, whereas this surface was decreased in the group treated with BMSCs, +28.4 versus −26.4 %, respectively (p = 0.01). BMSC seeding resulted in intimal hyperplasia with cell colonization and disappearance of the thrombus.

Conclusion

In conclusion, percutaneous seeding of BMSCs may colonize and heal the arterial wall thus limiting aneurysm expansion.

Abruzzo T, Tun T, Sambanis A (2007) Efficient transmicrocatheter delivery of functional fibroblasts with a bioengineered collagen gel-platinum microcoil complex: toward the development of endovascular cell therapy for cerebral aneurysms. AJNR Am J Neuroradiol 28:1586–1593CrossRefPubMed

Allaire E, Muscatelli-Groux B, Guinault AM et al (2004) Vascular smooth muscle cell endovascular therapy stabilizes already developed aneurysms in a model of aortic injury elicited by inflammation and proteolysis. Ann Surg 239:417–427CrossRefPubMed

Altes TA, Cloft HJ, Short JG et al (2000) 1999 ARRS Executive Council Award. Creation of saccular aneurysms in the rabbit: a model suitable for testing endovascular devices. American Roentgen Ray Society. AJR Am J Roentgenol 174:349–354CrossRefPubMed

Anidjar S, Dobrin PB, Chejfec G et al (1994) Experimental study of determinants of aneurysmal expansion of the abdominal aorta. Ann Vasc Surg 8:127–136CrossRefPubMed

Anidjar S, Salzmann JL, Gentric D et al (1990) Elastase-induced experimental aneurysms in rats. Circulation 82:973–981CrossRefPubMed

Augello A, De Bari C (2010) The regulation of differentiation in mesenchymal stem cells. Hum Gene Ther 21:1226–1238CrossRefPubMed

Ausman JI (1997) The future of neurovascular surgery. Part I: Intracranial aneurysms. Surg Neurol 48:98–100CrossRefPubMed

Bouzeghrane F, Naggara O, Kallmes DF et al (2010) In vivo experimental intracranial aneurysm models: a systematic review. AJNR Am J Neuroradiol 31:418–423CrossRefPubMed

Cloft HJ (2007) HydroCoil for Endovascular Aneurysm Occlusion (HEAL) study: 3–6 month angiographic follow-up results. AJNR Am J Neuroradiol 28:152–154PubMed

10.

Dai D, Ding YH, Danielson MA et al (2005) Histopathologic and immunohistochemical comparison of human, rabbit, and swine aneurysms embolized with platinum coils. AJNR Am J Neuroradiol 26:2560–2568PubMed

11.

Dai D, Ding YH, Lewis DA et al (2006) A proposed ordinal scale for grading histology in elastase-induced, saccular aneurysms. AJNR Am J Neuroradiol 27:132–138PubMed

12.

Delbosc S, Alsac JM, Journe C et al (2011) Porphyromonas gingivalis participates in pathogenesis of human abdominal aortic aneurysm by neutrophil activation. Proof of concept in rats. PLoS One 6:e18679CrossRefPubMed

13.

Dunsmore SE, Shapiro SD (2004) The bone marrow leaves its scar: new concepts in pulmonary fibrosis. J Clin Invest 113:180–182PubMed

14.

Frosen J, Piippo A, Paetau A et al (2004) Remodeling of saccular cerebral artery aneurysm wall is associated with rupture: histological analysis of 24 unruptured and 42 ruptured cases. Stroke 35:2287–2293CrossRefPubMed

15.

Frosen J, Tulamo R, Paetau A et al (2012) Saccular intracranial aneurysm: pathology and mechanisms. Acta Neuropathol 123(6):773–786CrossRefPubMed

16.

Gomes D, Louedec L, Plissonnier D et al (2001) Endoluminal smooth muscle cell seeding limits intimal hyperplasia. J Vasc Surg 34:707–715CrossRefPubMed

17.

Hayakawa M, Murayama Y, Duckwiler GR et al (2000) Natural history of the neck remnant of a cerebral aneurysm treated with the Guglielmi detachable coil system. J Neurosurg 93:561–568CrossRefPubMed

18.

Ingber DE (2008) Tensegrity-based mechanosensing from macro to micro. Prog Biophys Mol Biol 97:163–179CrossRefPubMed

19.

Kawakami O, Miyamoto S, Hatano T et al (2005) Accelerated embolization healing of aneurysms by polyethylene terephthalate coils seeded with autologous fibroblasts. Neurosurgery 56:1075–1081, discussion 1075-1081PubMed

20.

Kazi M, Thyberg J, Religa P et al (2003) Influence of intraluminal thrombus on structural and cellular composition of abdominal aortic aneurysm wall. J Vasc Surg 38:1283–1292CrossRefPubMed

21.

Kimchi TJ, Willinsky RA, Spears J et al (2007) Endovascular treatment of intracranial aneurysms with matrix coils: immediate posttreatment results, clinical outcome and follow-up. Neuroradiology 49:223–229CrossRefPubMed

22.

King PS, Cooperberg PL, Madigan SM (1986) The anechoic crescent in abdominal aortic aneurysms: not a sign of dissection. AJR Am J Roentgenol 146:345–348CrossRefPubMed

23.

Mazighi M, Tchetche D, Goueffic Y et al (2006) Percutaneous transplantation of genetically-modified autologous fibroblasts in the rabbit femoral artery: a novel approach for cardiovascular gene therapy. J Vasc Surg 44:1067–1075CrossRefPubMed

24.

Mehard WB, Heiken JP, Sicard GA (1994) High-attenuating crescent in abdominal aortic aneurysm wall at CT: a sign of acute or impending rupture. Radiology 192:359–362PubMed

25.

Michel JB, Martin-Ventura JL, Egido J et al (2011) Novel aspects of the pathogenesis of aneurysms of the abdominal aorta in humans. Cardiovasc Res 90:18–27CrossRefPubMed

26.

Molyneux A, Kerr R, Stratton I et al (2002) International Subarachnoid Aneurysm Trial (ISAT) of neurosurgical clipping versus endovascular coiling in 2143 patients with ruptured intracranial aneurysms: a randomised trial. Lancet 360:1267–1274CrossRefPubMed

27.

Murayama Y, Vinuela F, Suzuki Y et al (1997) Ion implantation and protein coating of detachable coils for endovascular treatment of cerebral aneurysms: concepts and preliminary results in swine models. Neurosurgery 40:1233–1243, discussion 1243-1234CrossRefPubMed

28.

Niimi Y, Song J, Madrid M et al (2006) Endosaccular treatment of intracranial aneurysms using matrix coils: early experience and midterm follow-up. Stroke 37:1028–1032CrossRefPubMed

29.

Ollivier V, Faure S, Tarantino N et al (2003) Fractalkine/CX3CL1 production by human aortic smooth muscle cells impairs monocyte procoagulant and inflammatory responses. Cytokine 21:303–311CrossRefPubMed

30.

Onizuka M, Miskolczi L, Gounis MJ et al (2006) Elastase-induced aneurysms in rabbits: effect of postconstruction geometry on final size. AJNR Am J Neuroradiol 27:1129–1131PubMed

31.

Plissonnier D, Henaff M, Poncet P et al (2000) Involvement of antibody-dependent apoptosis in graft rejection. Transplantation 69:2601–2608CrossRefPubMed

32.

Raymond J, Desfaits AC, Roy D (1999) Fibrinogen and vascular smooth muscle cell grafts promote healing of experimental aneurysms treated by embolization. Stroke 30:1657–1664CrossRefPubMed

33.

Raymond J, Guilbert F, Weill A et al (2003) Long-term angiographic recurrences after selective endovascular treatment of aneurysms with detachable coils. Stroke 34:1398–1403CrossRefPubMed

34.

Raymond J, Roy D (1997) Safety and efficacy of endovascular treatment of acutely ruptured aneurysms. Neurosurgery 41:1235–1245, discussion 1245-1236CrossRefPubMed

35.

Raymond J, Roy D, Bojanowski M et al (1997) Endovascular treatment of acutely ruptured and unruptured aneurysms of the basilar bifurcation. J Neurosurg 86:211–219CrossRefPubMed

36.

Raymond J, Venne D, Allas S et al (1999) Healing mechanisms in experimental aneurysms. I. Vascular smooth muscle cells and neointima formation. J Neuroradiol 26:7–20PubMed

37.

Satta J, Laara E, Juvonen T (1996) Intraluminal thrombus predicts rupture of an abdominal aortic aneurysm. J Vasc Surg 23:737–739CrossRefPubMed

38.

Sherman LS, Munoz J, Patel SA et al (2011) Moving from the laboratory bench to patients’ bedside: considerations for effective therapy with stem cells. Clin Transl Sci 4:380–386CrossRefPubMed

39.

Short JG, Fujiwara NH, Marx WF et al (2001) Elastase-induced saccular aneurysms in rabbits: comparison of geometric features with those of human aneurysms. AJNR Am J Neuroradiol 22:1833–1837PubMed

40.

Siegel CL, Cohan RH, Korobkin M et al (1994) Abdominal aortic aneurysm morphology: CT features in patients with ruptured and nonruptured aneurysms. AJR Am J Roentgenol 163:1123–1129CrossRefPubMed

41.

Venne D, Raymond J, Allas S et al (1999) Healing of experimental aneurysms. II: Platelet extracts can increase the thickness of the neointima at the neck of treated aneurysms. J Neuroradiol 26:92–100PubMed

42.

Wolf YG, Thomas WS, Brennan FJ et al (1994) Computed tomography scanning findings associated with rapid expansion of abdominal aortic aneurysms. J Vasc Surg 20:529–535, discussion 535-528CrossRefPubMed

Titel: Autologous mesenchymal stem cell endografting in experimental cerebrovascular aneurysms
verfasst von: Aymeric Rouchaud
Clément Journé
Liliane Louedec
Véronique Ollivier
Mohammed Derkaoui
Jean-Baptiste Michel
Mikael Mazighi
Publikationsdatum: 01.06.2013
Verlag: Springer-Verlag
Erschienen in: Neuroradiology / Ausgabe 6/2013
Print ISSN: 0028-3940
Elektronische ISSN: 1432-1920
DOI: https://doi.org/10.1007/s00234-013-1167-4

Leitlinien kompakt für die Neurologie

Mit medbee Pocketcards sicher entscheiden.

^{Seit 2022 gehört die medbee GmbH zum Springer Medizin Verlag}

Kostenlos registrieren

Neu im Fachgebiet Neurologie

Sozialer Aufstieg verringert Demenzgefahr

24.05.2024 Demenz Nachrichten

Ein hohes soziales Niveau ist mit die beste Versicherung gegen eine Demenz. Noch geringer ist das Demenzrisiko für Menschen, die sozial aufsteigen: Sie gewinnen fast zwei demenzfreie Lebensjahre. Umgekehrt steigt die Demenzgefahr beim sozialen Abstieg.

Hirnblutung unter DOAK und VKA ähnlich bedrohlich

17.05.2024 Direkte orale Antikoagulanzien Nachrichten

Kommt es zu einer nichttraumatischen Hirnblutung, spielt es keine große Rolle, ob die Betroffenen zuvor direkt wirksame orale Antikoagulanzien oder Marcumar bekommen haben: Die Prognose ist ähnlich schlecht.

Was nützt die Kraniektomie bei schwerer tiefer Hirnblutung?

17.05.2024 Hirnblutung Nachrichten

Eine Studie zum Nutzen der druckentlastenden Kraniektomie nach schwerer tiefer supratentorieller Hirnblutung deutet einen Nutzen der Operation an. Für überlebende Patienten ist das dennoch nur eine bedingt gute Nachricht.

Thrombektomie auch bei großen Infarkten von Vorteil

16.05.2024 Ischämischer Schlaganfall Nachrichten

Auch ein sehr ausgedehnter ischämischer Schlaganfall scheint an sich kein Grund zu sein, von einer mechanischen Thrombektomie abzusehen. Dafür spricht die LASTE-Studie, an der Patienten und Patientinnen mit einem ASPECTS von maximal 5 beteiligt waren.

Update Neurologie

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert.

Newsletter bestellen

Live-Webinar "Urologie und Sexualmedizin in der Praxis"

Springer Medizin

Abstract

Introduction

Methods

Results

Conclusion

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 6/2013

Correlation between pulmonary function and brain volume in healthy elderly subjects

MR imaging and differentiation of cerebral fat embolism syndrome from diffuse axonal injury: application of diffusion tensor imaging

3D neuromelanin-sensitive magnetic resonance imaging with semi-automated volume measurement of the substantia nigra pars compacta for diagnosis of Parkinson’s disease

Selective transarterial embolization with n-butyl-2-cyanoacrylate for the treatment of arterial hemorrhage after third molar extraction

3rd Spine Radiology