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Archives of Virology

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01.05.2024 | Annotated Sequence Record

Complete genome analysis of a novel narnavirus in sweet viburnum (Viburnum odoratissimum)

verfasst von: Yangwei Gao, Zhongtian Xu, Ping Li, Xiaodi Hu, Jian-Ping Chen, Chuan-Xi Zhang, Yiyuan Li

Erschienen in: Archives of Virology | Ausgabe 5/2024

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Abstract

Trees and shrubs provide important ecological services. However, few studies have surveyed the virome in trees and shrubs. In this study, we discovered a new positive-sense RNA virus originating from Viburnum odoratissimum, which we named "Vo narna-like virus". The complete genome of Vo narna-like virus is 3,451 nt in length with an open reading frame (ORF) encoding the RNA-dependent RNA polymerase (RdRP) protein. Phylogenetic analysis placed this virus within the betanarnavirus clade, sharing 53.63% amino acid sequence identity with its closest relative, Qingdao RNA virus 2. The complete sequence of the virus was confirmed by rapid amplification of cDNA ends (RACE) and Sanger sequencing. Small interfering RNA (siRNA) analysis indicated that this virus interacts with the RNA interference (RNAi) pathway of V. odoratissimum. This is the first report of a narnavirus in V. odoratissimum.

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Titel: Complete genome analysis of a novel narnavirus in sweet viburnum (Viburnum odoratissimum)
verfasst von: Yangwei Gao
Zhongtian Xu
Ping Li
Xiaodi Hu
Jian-Ping Chen
Chuan-Xi Zhang
Yiyuan Li
Publikationsdatum: 01.05.2024
Verlag: Springer Vienna
Erschienen in: Archives of Virology / Ausgabe 5/2024
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-024-06000-y

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Complete genome analysis of a novel narnavirus in sweet viburnum (Viburnum odoratissimum)

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