Introduction
Cardiovascular disease (CVD) is the most prevalent morbidity and the main cause of mortality in subjects with type 2 diabetes (T2D). It has been estimated that CVD may develop in about 32% of all subjects with T2D with coronary artery disease (CAD) and cerebrovascular accident (CVA) as the main causes of death [
1]. Some studies indicate that CVD death in the context of T2D is more prevalent in low and middle income communities [
2]. Though persistent high blood glucose is definitely a contributor to CVD risk, there may also be some other predisposing factors like deranged blood lipids [
3]. Cardiometabolic risk factors including abdominal adiposity, dyslipidemia and high blood pressure all contribute to development of diabetes and its further complications [
4]. Fortunately, most of these are modifiable and that is why having a healthy diet and life style is the main step in prevention and treatment of T2D [
5,
6].
Dietary fats, as one of the main dietary components, may affect most, if not all, cardiometabolic risk factors including body weight [
7,
8], blood glucose [
9,
10] and lipids [
11,
12]. A huge body of evidence indicates that replacement of saturated fats with liquid plant oils containing mono- and polyunsaturated fatty acids (MUFAs and PUFAs, respectively) may be accompanied by reduced risk of CVD [
13], the primary cause of mortality in T2D [
2].
Healthy plant oils may contain phytochemicals, naturally occurring bioactive compounds carrying various health benefits including antidiabetic effects [
14]. One of the phytochemicals recently received huge attention is γ-oryzanol (ORZ), a phytochemical of rice bran oil. An increasing body of evidence indicates the beneficial effects of ORZ on cardiometabolic risk factors including increased body weight/fat [
15,
16], deranged blood lipids [
17,
18], raised blood pressure [
19] and dysglycemia [
18,
20]. As a result, ORZ has been considered as an adjunct approach to the currently recommended life style modifications to prevent and treat T2D and its complications [
21‐
23]. Notwithstanding, the effects of daily intake of ORZ under free living conditions with no predetermined intake dose on cardiometabolic biomarkers in T2D have not been evaluated to date.
This study was undertaken to examine the effects of daily consumption of ORZ-fortified canola oil, as compared with plain canola and sunflower oils, on certain cardiometabolic biomarkers. We chose canola oil as a vehicle for ORZ fortification because: (i) oils as staple foods in Iran are suitable for fortification purposes [
24,
25], and (ii) canola oil containing 60% oleic acid (C18:1), 20% linolenic acid (C18:2) and 10% α-linolenic acid (C18:3) is one of the healthiest oils [
26]. We used sunflower oil to compare the effects of ORZ-fortified and –unfortified canola oils with because (i) it is the most common liquid plant oil used in Iran; and (ii) having about 85% unsaturated fatty acids including linoleic and oleic acids, it is also considered a healthy oil [
27].
As most liquid cooking oils available in the Iranian market are voluntarily fortified with vitamins A and D, all oils used in this study contained equal amounts of these two vitamins. Nevertheless, we refer to the canola oil without ORZ as “unfortified” throughout this article just for convenience.
Results
Ninety-two subjects from both sexes aged 57.6 ± 1.9 y completed the study. There were no significant between-group differences in demographic variables or the amount of consumed oil per capita (Table
1). There were no complaints of the organoleptic properties of the oils and nor was there any attrition throughout the intervention period indicating that the compliance was 100%. Dietary intake data showed no statistically significant inter- or intra-group differences (Table
2).
Table 1
Age, sex and per capita oil consumption of the studied groups
Age (y) | 53.9 ± 1.9 | 58.7 ± 1.8 | 60.1 ± 1.9 | 0.064 |
Sex: No (%) |
Male | 17 (56.7) | 20 (62.5) | 17 (56.7) | 0.864 |
Female | 13 (43.3) | 12 (37.5) | 13 (43.3) |
Per capita oil consumption (g/d)* | 29.1 ± 0.7 | 28.7 ± 0.8 | 30.2 ± 0.4 | 0.367 |
Table 2
Intra- and inter-group comparisons of mean daily energy and some selected nutrients intakes
Energy (kcal) | 1682.2 ± 99.6 | 1738.8 ± 126.4 | 0.610 | 1662.4 ± 63.0 | 1801.3 ± 98.2 | 0.130 | 1718.6 ± 65.1 | 1695.7 ± 107.0 | 0.838 | 0.828 | 0.680 | 0.357 |
Carbohydrate (g) | 246.9 ± 20.3 | 306.4 ± 57.7 | 0.263 | 255.4 ± 13.5 | 271.3 ± 18.2 | 0.271 | 261.2 ± 11.1 | 259.5 ± 17.0 | 0.926 | 0.624 | 0.461 | 0.947 |
Protein (g) | 64.1 ± 3.2 | 64.4 ± 5.0 | 0.953 | 62.6 ± 3.0 | 70.0 ± 4.8 | 0.139 | 61.1 ± 3.2 | 60.2 ± 3.7 | 0.405 | 0.579 | 0.661 | 0.182 |
Fat (g) | 51.6 ± 3.4 | 50.4 ± 4.0 | 0.775 | 46.2 ± 3.2 | 46.9 ± 3.3 | 0.820 | 48.8 ± 2.9 | 45.7 ± 3.5 | 0.311 | 0.995 | 0.726 | 0.752 |
Total dietary fiber (g) | 15.2 ± 1.2 | 14.2 ± 1.3 | 0.381 | 15.3 ± 0.8 | 16.3 ± 1.2 | 0.514 | 12.7 ± 1.2 | 14.6 ± 2.0 | 0.440 | 0.588 | 0.828 | 0.939 |
Vitamin A (μg) | 753.5 = 131.2 | 573.0 ± 115.9 | 0.272 | 569.6 ± 97.0 | 419.5 ± 41.5 | 0.174 | 807.4 ± 198.4 | 519.6 ± 72.3 | 0.159 | 0.452 | 0.883 | 0.779 |
Iron (mg) | 13.8 ± 1.0 | 13.7 ± 1.1 | 0.983 | 14.3 ± 0.6 | 16.1 ± 1.4 | 0.242 | 13.2 ± 0.7 | 14.9 = 2.5 | 0.311 | 0.622 | 0.735 | 0.991 |
Zinc (mg) | 6.4 ± 0.4 | 5.8 ± 0.4 | 0.315 | 5.7 ± 0.3 | 6.5 ± 0.8 | 0.349 | 6.6 ± 0.3 | 5.5 ± 0.7 | 0.153 | 0.509 | 0.907 | 0.816 |
Calcium (mg) | 572.0 ± 40.2 | 564.3 ± 44.4 | 0.869 | 576.4 ± 43.3 | 591.4 ± 41.0 | 0.748 | 574.7 ± 37.8 | 571.6 ± 50.9 | 0.562 | 0.902 | 0.531 | 0.303 |
Table
3 demonstrates changes of the studied cardiometabolic markers in the three groups. Intra-group changes of BMI showed a significant decrease in all three groups and there was no significant inter-group difference. Notwithstanding, WC decreased significantly only in two canola consuming groups and the changes were statistically significant as compared with group 3 (sunflower oil). Interestingly, both systolic and diastolic blood pressure (SBP and DBP, respectively) reduced significantly just in Group 1. In between-group comparisons, SBP changes were significantly different as compared with group 3 (p = 0.049) and this difference remained statistically significant after adjustment for final values of BMI (p = 0.049) but disappeared after adjustment for WC final values (p = 0.076).
Table 3
Intra- and inter-group comparisons of anthropometric, blood pressure and biochemical biomarkers
BMI (kg/m2) | 28.4 ± 0.79 | 27.9 ± 0.77 | < 0.001 | 29.7 ± 0.72 | 28.3 ± 0.71 | < 0.001 | 29.2 ± 0.68 | 28.5 ± 0.72 | 0.019 | 0.831 | 0.753 | 0.390 |
WC (cm) | 99.7 ± 2.0 | 97.03 ± 2.0 | < 0.001 | 104.6 ± 1.9 | 102.4 ± 1.9 | < 0.001 | 102.7 ± 1.4 | 102.2 ± 1.4 | 0.472 | 0.894 | 0.017 | 0.047 |
SBP (mmHg) | 135.5 ± 2.6 | 127.9 ± 2.7 | 0.035 | 139.6 ± 2.9 | 134.0 ± 2.4 | 0.078 | 141.9 ± 3.8 | 140.8 ± 4.3 | 0.661 | 0.581 | 0.049 | 0.306 |
DBP (mmHg) | 84.7 ± 1.6 | 76.3 ± 3.0 | 0.0175 | 85.6 ± 1.4 | 83.9 ± 1.6 | 0.266 | 86.3 ± 1.5 | 84.4 ± 2.0 | 0.315 | 0.057 | 0.059 | 0.997 |
FBG (mg/dL) | 134.0 ± 6.2 | 126.4 ± 5.8 | 0.039 | 157.1 ± 13.8 | 141.2 ± 11.1 | 0.090 | 144.6 ± 8.1 | 152.4 ± 11.6 | 0.367 | 0.990 | 0.157 | 0.113 |
HbA1c (%) | 6.1 ± 0.12 | 5.4 ± 0.12 | < 0.001 | 6.1 ± 0.24 | 5.8 ± 0.20 | 0.053 | 5.9 ± 0.18 | 6.0 ± 0.20 | 0.472 | 0.010 | < 0.001 | 0.093 |
Insulin (μIU/mL) | 17.2 ± 2.2 | 16.2 ± 1.9 | 0.437 | 16.6 ± 2.1 | 16.5 ± 1.8 | 0.942 | 16.9 ± 2.2 | 18.3 ± 2.2 | 0.343 | 0.893 | 0.380 | 0.640 |
HOMA-IR | 5.6 ± 0.7 | 4.9 ± 0.6 | 0.168 | 5.7 ± 0.7 | 5.5 ± 0.6 | 0.239 | 5.7 ± 0.8 | 6.5 ± 0.8 | 0.228 | 0.976 | 0.079 | 0.118 |
TG (mg/dL) | 134.7 ± 9.8 | 116.8 ± 7.8 | 0.005 | 147.1 ± 11.8 | 129.8 ± 10.5 | 0.141 | 131.1 ± 10.5 | 115.7 ± 7.7 | 0.070 | 0.776 | 0.997 | 0.815 |
TC (mg/dL) | 145.1 ± 5.5 | 142.8 ± 6.9 | 0.535 | 162.2 ± 7.3 | 159.8 ± 7.7 | 0.592 | 150.6 ± 5.6 | 149.0 ± 6.8 | 0.785 | 0.949 | 0.976 | 0.994 |
HDL-C (mg/dL) | 58.9 ± 2.8 | 58.3 ± 2.8 | 0.795 | 59.1 ± 3.3 | 57.0 ± 2.5 | 0.489 | 62.5 ± 2.4 | 58.7 ± 2.4 | 0.156 | 0.894 | 0.947 | 0.991 |
LDL-C (mg/dL) | 100.3 ± 3.6 | 100.9 ± 4.1 | 0.850 | 109.3 ± 6.4 | 105.3 ± 6.7 | 0.388 | 100.2 ± 5.1 | 102.9 ± 5.9 | 0.597 | 0.919 | 0.937 | 0.740 |
FSG concentrations decreased in both Groups 1 and 2 but only in Group 1 was the decrement statistically significant. In Group 3, there was an insignificant increase in FSG. Similarly, HbA1c showed a decrease in both canola consuming groups which was significant in Group 1 (6.1 ± 0.12 vs. 5.4 ± 0.12% p < 0.001) and close to significant in group 2 (6.1 ± 0.24 vs. 5.8 ± 0.20%, p = 0.053). HbA1c did not change in Group 3. Other biomarkers of glycemic status including fasting serum insulin and HOMA-IR did not show any significant intra- or inter-group difference. Among the components of serum lipid profile, only serum TG showed a reduction in all three groups which was statistically significant just in Group 1 (134.7 ± 9.8 vs. 116.8 ± 7.8 mg/dL, p = 0.005).
Discussion
We found a significant decrease in BMI following 12 weeks intervention in all three groups. Though consumption of canola oil may bring about a slight weight reduction [
31], it is plausible that this weight reduction have been resulted from unconsciously paying more attention to the amount of dietary, including oil, intake in all three groups. To support this notion, dietary intake data did not show any significant intra- or inter-group changes. Significant decrement of WC in just two canola-consuming groups deserves great attention. The effect of canola oil consumption on WC has already been reported and has been suggested to be due to its oleic acid content [
32]. Nevertheless, addition of ORZ did not potentiate the effect of canola oil on WC.
The effect of ORZ-fortified canola oil consumption on blood pressure is noteworthy. Some evidence indicates the reducing effect of canola oil on blood pressure through reduction of visceral fat [
32]. However, absence of any significant change in blood pressure in Group 2 suggests the effect of added ORZ. In support of this notion, a clinical trial failed to show any significant change in blood pressure of adult subjects with T2D who consumed 30 g/d canola oil for eight weeks [
33]. Removal of significant difference of SBP between groups 1 and 3 in the current study indicates that the effect of ORZ on SBP is, at least in part, mediated through reduction of WC and independent of BMI. Along the same line, in a population-based study a direct relationship between blood pressure and WC, independent of BMI, was observed [
34]. Current evidence on the effect of ORZ on blood pressure is scarce. In an experimental study, the reducing effect of rice bran extract (RBE) on blood pressure of hypertensive rats was documented [
35]. Notwithstanding, this effect might have been due to ferulic acid in RBE, that may decrease blood pressure via induction of nitric oxide (NO) generation in blood vessels [
36]. It is worth to note that the majority of our participants were normotensive and hence might be less responsive to the effect of ORZ on blood pressure. The effect of ORZ on blood pressure deserves further studies.
Twelve weeks consumption of ORZ-fortified canola oil resulted in a significant decrease in both FBG and HbA1c in adult subjects with T2D. However, insulin resistance, as judged by HOMA-IR, did not change significantly. Though similar changes were observed in unfortified canola oil group, they were not statistically significant. Studies on the effect of canola oil on glycemic indicators are scarce. A clinical trial on the effects of eight weeks consumption of 30 g/d canola or olive oil, as compared with sunflower oil, failed to show any significant improvement in FBG and serum insulin concentrations in adult subjects with T2D [
33]. A meta-analytical study also reported almost similar findings [
31]. In contrast, in a study reported from India, six moths consumption of canola oil resulted in a significant decrease in FBG in adult subjects with non-alcoholic fatty liver (NAFLD) [
37]. Canola oil is a rich source of MUFAs, whose ameliorating effect on HbA1c has been demonstrated by a meta-analysis study [
38]. Altogether, it is likely that duration of consumption is a crucial determinant of the effect of canola oil on glycemic status. It is noteworthy that both canola-consuming groups had a significant decrease in BMI. A recent meta-analysis study reported the effect of canola oil consumption on weight reduction [
31]. It is, therefore, possible that the improvement of glycemic biomarkers had been due to decrement of BMI. It has been estimated that for each kilogram weight loss, there may be 0.1% decrease in HbA1c [
39]. In the current study, both canola-consuming groups (groups 1 and 2) had almost 1.2 kg weight loss, which was accompanied by about 10% reduction in HbA1c. This reduction was significantly more in Group 1 than in group 2 indicating the synergistic and weight-independent effect of ORZ and canola oil on HbA1c.
Serum TG concentrations in all three groups decreased but only in group 1 (canola oil + ORZ) was this decrement statistically significant. Other components of serum lipid profile did not change significantly in either group. Contrary to our finding, improvement of serum lipid profile was observed in a large multi-center clinical trial [
40]. Though serum lipid profile, notably TG concentrations, may improve following weight loss [
41], since there was a similar weight loss in both canola consuming groups, reduction of serum TG concentrations only in Group 1 was likely due to the added ORZ. The effect of ORZ intake on serum TG concentrations has already been demonstrated in animal model [
20]. Notwithstanding, in a controlled clinical trial on dyslipidemic subjects, consumption of rice bran oil containing ORZ for four weeks did not significantly affect lipid profile components but serum LDL-C concentration [
17]. Experimental studies indicate that ORZ, through inhibition of stearyl CoA saturase, impedes TG accumulation in the liver [
42].
To the best of our knowledge, this is the first clinical trial of the effectiveness of daily consumption of ORZ-fortified canola oil, as compared with unfortified canola and sunflower oils, on anthropometric, glycemic and lipidemic biomarkers of the adult subjects with T2D. It is noteworthy that in this study, unlike similar works, there was no predetermined amount of the oils to be consumed. Nevertheless, some limitations are acknowledged. Our dietary intake assessment using 24 h dietary recall for two days failed to show any changes despite significant reduction of BMI in the three studied groups. It has been recommended to repeat 24 h recall for 4 to eight days to show the variations [
43]. Nevertheless, several repetition of 24 h recall might limit the participation and resulting in selection bias [
44]. In addition, having a control group with no intervention would help better interpretation of the findings. However, blinding for a group of subjects with no intervention would be impossible.
In conclusion, replacing common cooking oils, including animal oils, with either sunflower or canola oils for 12 weeks improved certain studied biomarkers notably BMI, WC and serum TG concentrations. However, only ORZ-fortified canola oil resulted in a significant decrease of blood pressure and glycemic biomarkers, notably FBG and HbA1c. These findings can help both clinicians and public health authorities for dietary recommendations to subjects with T2D and presumably the whole community.
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