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International Orthopaedics

Erschienen in:

01.10.2012 | Original Paper

Expression and significance of Kruppel-like factor 6 gene in osteosarcoma

verfasst von: Kai Chen, Yu Chen, Xiao-dong Zhu, Yu-shu Bai, Xian-zhao Wei, Chuan-feng Wang, Zi-qiang Chen, Ming Li

Erschienen in: International Orthopaedics | Ausgabe 10/2012

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Abstract

Purpose

Osteosarcoma is primary malignant tumour of bone. Kruppel-like factor 6 (KLF6) is a tumor suppressor gene frequently inactivated in a number of human cancers and a ubiquitously expressed zinc-finger transcription factor. The present study aimed to first explore the relationship between the expression level of the KLF6 gene in osteosarcoma and the occurrence of bone tumours.

Methods

KLF6 mRNA and protein expression levels in osteosarcoma and normal bone tissue were assayed by real-time quantitative PCR and immunohistochemistry. KLF6 mRNA and protein expression levels in osteosarcoma cells and normal osteoblasts were detected by semi-quantitative reverse transcription PCR and Western blotting, respectively.

Results

Both the expression of KLF6 mRNA and protein in osteosarcoma cells and tissues were significantly lower than that in normal cells and tumour-adjacent tissues.

Conclusions

KLF6 is a putative tumor suppressor gene involved in osteosarcoma which can be used as a new therapeutic target and an important marker for early diagnosis and postoperative monitoring.

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Titel: Expression and significance of Kruppel-like factor 6 gene in osteosarcoma
verfasst von: Kai Chen
Yu Chen
Xiao-dong Zhu
Yu-shu Bai
Xian-zhao Wei
Chuan-feng Wang
Zi-qiang Chen
Ming Li
Publikationsdatum: 01.10.2012
Verlag: Springer-Verlag
Erschienen in: International Orthopaedics / Ausgabe 10/2012
Print ISSN: 0341-2695
Elektronische ISSN: 1432-5195
DOI: https://doi.org/10.1007/s00264-012-1626-2

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Expression and significance of Kruppel-like factor 6 gene in osteosarcoma