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Digestive Diseases and Sciences

Erschienen in:

06.03.2024 | Original Article

Gastrointestinal Characteristics of Constipation from the Perspectives of Microbiome and Metabolome

verfasst von: Huimin Wang, Di Zhao, Senyan Wang, Huanle Liu, Sijun Zhao, Zhenyu Li, Xuemei Qin, Xiaojie Liu

Erschienen in: Digestive Diseases and Sciences | Ausgabe 4/2024

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Abstract

Background

Constipation is one of the most common gastrointestinal complaints. Yet, the underlying mechanisms of constipation remain to be explored deeply. Integration of microbiome and metabolome is powerful and promising to demonstrate characteristics of constipation.

Aim of Study

This study aimed to characterize intestinal microbiome and metabolome of constipation. In addition, this study revealed the correlations among behaviors, intestinal microbiota, and metabolites interrupted by constipation.

Methods

Firstly, the constipation model was successfully applied. At the macro level, the ability of learning, memory, locomotor activity, and the defecation index of rats with constipation-like phenotype were characterized. At the micro-level, 16S rRNA sequencing was applied to analyze the intestinal microbiota in rats with constipation-like phenotype. ¹H nuclear magnetic resonance (NMR)-based metabolomics was employed to investigate the metabolic phenotype of constipation. In addition, we constructed a correlation network, intuitively showing the correlations among behaviors, intestinal microbiota, and metabolites.

Results

Constipation significantly attenuated the locomotor activity, memory recognition, and frequency of defecation of rats, while increased the time of defecation. Constipation significantly changed the diversity of intestinal microbial communities, which correspondingly involved in 5 functional pathways. Besides, 28 fecal metabolites were found to be associated with constipation, among which 14 metabolites were further screened that can be used to diagnose constipation. On top of this, associated networks intuitively showed the correlations among behaviors, intestinal microbiota, and metabolites.

Conclusions

The current findings are significant in terms of not only laying a foundation for understanding characteristics of constipation, but also providing accurate diagnosis and treatments of constipation clinically.

Graphical Abstract

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Titel: Gastrointestinal Characteristics of Constipation from the Perspectives of Microbiome and Metabolome
verfasst von: Huimin Wang
Di Zhao
Senyan Wang
Huanle Liu
Sijun Zhao
Zhenyu Li
Xuemei Qin
Xiaojie Liu
Publikationsdatum: 06.03.2024
Verlag: Springer US
Erschienen in: Digestive Diseases and Sciences / Ausgabe 4/2024
Print ISSN: 0163-2116
Elektronische ISSN: 1573-2568
DOI: https://doi.org/10.1007/s10620-024-08334-8

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Abstract

Background

Aim of Study

Methods

Results

Conclusions

Graphical Abstract

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