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09.09.2023 | Original Article

Gut microbiome in men with chronic prostatitis/chronic pelvic pain syndrome: profiling and its predictive significance

verfasst von: Shuai Wang, Maolin Zang, Xiaohui Yang, Linchen Lv, Lipeng Chen, Jianfeng Cui, Yaxiao Liu, Yangyang Xia, Nan Zhou, Zizhuo Yang, Yan Li, Benkang Shi

Erschienen in: World Journal of Urology | Ausgabe 11/2023

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Abstract

Purpose

To investigate the difference in gut microbiome composition between patients with chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) and healthy controls, and to assess the potential of gut microbiota as predictive markers for CP/CPPS risk.

Methods

The present study included 41 CP/CPPS patients and 43 healthy controls in China. Fecal specimen data were obtained and analysed using 16S rRNA gene sequencing. Alpha and beta-diversity indices, relative microbiome abundances, cluster analysis, and linear discriminant analysis effect size (LEfSe) were employed. Microbial biomarkers were selected for the development of a diagnostic classification model, and the functional prediction was conducted using PICRUSt2.

Results

Alpha-diversity measures revealed no statistically significant difference in bacterial community structure between CP/CPPS patients and controls. However, significant differences were observed in the relative abundances of several bacterial genera. Beta-diversity analysis revealed a distinct separation between the two groups. Significant inter-group differences were noted at various taxonomic levels, with specific bacterial genera being significantly different in abundance. The LEfSe analysis indicated that three bacterial species were highly representative and seven bacterial species were low in CP/CPPS patients as compared to the control group. A diagnostic model for CP/CPPS based on microbial biomarkers exhibited good performance. PICRUSt2 functional profiling indicated significant differences in the development and regeneration pathway.

Conclusion

Significant differences in the gut microbiome composition were found between groups. The study provided a novel diagnostic model for CP/CPPS based on microbiota, presenting promising potential for future therapeutic targets and non-invasive diagnostic biomarkers for CP/CPPS patients.

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Collins MM, Stafford RS, O’Leary MP, Barry MJ (1998) How common is prostatitis? A national survey of physician visits. J Urol 159:1224–1228CrossRefPubMed

Habermacher GM, Chason JT, Schaeffer AJ (2006) Prostatitis/chronic pelvic pain syndrome. Annu Rev Med 57:195–206CrossRefPubMed

Pena VN, Engel N, Gabrielson AT, Rabinowitz MJ, Herati AS (2021) Diagnostic and management strategies for patients with chronic prostatitis and chronic pelvic pain syndrome. Drugs Aging 38:845–886CrossRefPubMed

He H, Luo H, Qian B et al (2023) Autonomic nervous system dysfunction is related to chronic prostatitis/chronic pelvic pain syndrome. World J Mens Health. https://doi.org/10.5534/wjmh.220248CrossRefPubMed

Mayer EA, Tillisch K, Gupta A (2015) Gut/brain axis and the microbiota. J Clin Invest 125:926–938CrossRefPubMedPubMedCentral

Minerbi A, Shen S (2022) Gut microbiome in anesthesiology and pain medicine. Anesthesiology 137:93–108CrossRefPubMed

Shoskes DA, Wang H, Polackwich AS, Tucky B, Altemus J, Eng C (2016) Analysis of gut microbiome reveals significant differences between men with chronic prostatitis/chronic pelvic pain syndrome and controls. J Urol 196:435–441CrossRefPubMed

Vangay P, Johnson AJ, Ward TL et al (2018) US immigration westernizes the human gut microbiome. Cell 175(4):962–972CrossRefPubMedPubMedCentral

Weersma RK, Zhernakova A, Fu J (2020) Interaction between drugs and the gut microbiome. Gut 69:1510–1519CrossRefPubMed

10.

Wu Y, Jiang H, Tan M, Lu X (2020) Screening for chronic prostatitis pathogens using high-throughput next-generation sequencing. Prostate 80:577–587CrossRefPubMedPubMedCentral

11.

Lv J, Wang J, Yu Y et al (2023) Alterations of gut microbiota are associated with blood pressure: a cross-sectional clinical trial in Northwestern China. J Transl Med 30(21):429CrossRef

12.

Litwin MS, McNaughton-Collins M, Fowler FJ et al (1999) The National Institutes of Health chronic prostatitis symptom index: development and validation of a new outcome measure. Chronic prostatitis collaborative research network. J Urol 162:369–375CrossRefPubMed

13.

Franzosa EA, Morgan XC, Segata N et al (2014) Relating the metatranscriptome and metagenome of the human gut. Proc Natl Acad Sci U S A 3(111):E2329–E2338

14.

Willis AD (2019) Rarefaction, alpha diversity, and statistics. Front Microbiol 10:2407CrossRefPubMedPubMedCentral

15.

Goodrich JK, Di Rienzi SC, Poole AC et al (2014) Conducting a microbiome study. Cell 158:250–262CrossRefPubMedPubMedCentral

16.

Chang F, He S, Dang C (2022) Assisted selection of biomarkers by linear discriminant analysis effect size (LEfSe) in microbiome data. J Vis Exp. https://doi.org/10.3791/61715-vCrossRefPubMed

17.

Murugesan S, Al KS (2023) Salivary microbiome and hypertension in the Qatari population. J Transl Med 8(21):454CrossRef

18.

Yu S, Chen J, Zhao Y et al (2023) Oral-microbiome-derived signatures enable non-invasive diagnosis of laryngeal cancers. J Transl Med 5(21):438CrossRef

19.

Shoskes DA, Altemus J, Polackwich AS, Tucky B, Wang H, Eng C (2016) The urinary microbiome differs significantly between patients with chronic prostatitis/chronic pelvic pain syndrome and controls as well as between patients with different clinical phenotypes. Urology 92:26–32CrossRefPubMed

20.

Mändar R, Punab M, Korrovits P et al (2017) Seminal microbiome in men with and without prostatitis. Int J Urol 24:211–216CrossRefPubMed

21.

Cryan JF, Dinan TG (2012) Mind-altering microorganisms: the impact of the gut microbiota on brain and behaviour. Nat Rev Neurosci 13:701–712CrossRefPubMed

22.

Montagnani M, Bottalico L, Potenza MA et al (2023) The crosstalk between gut microbiota and nervous system: a bidirectional interaction between microorganisms and metabolome. Int J Mol Sci 24:10322CrossRefPubMedPubMedCentral

23.

Krogius-Kurikka L, Lyra A, Malinen E et al (2009) Microbial community analysis reveals high level phylogenetic alterations in the overall gastrointestinal microbiota of diarrhoea-predominant irritable bowel syndrome sufferers. BMC Gastroenterol 9:95CrossRefPubMedPubMedCentral

24.

Saulnier DM, Riehle K, Mistretta T-A et al (2011) Gastrointestinal microbiome signatures of pediatric patients with irritable bowel syndrome. Gastroenterology 141:1782–1791CrossRefPubMed

25.

Radjabzadeh D, Bosch JA, Uitterlinden AG et al (2022) Gut microbiome-wide association study of depressive symptoms. Nat Commun 13:7128CrossRefPubMedPubMedCentral

26.

Parker BJ, Wearsch PA, Veloo ACM, Rodriguez-Palacios A (2020) The genus alistipes: gut bacteria with emerging implications to inflammation, cancer, and mental health. Front Immunol 11:906CrossRefPubMedPubMedCentral

27.

Tan W, Zhang Q, Dong Z et al (2020) Phosphatidylcholine ameliorates LPS-induced systemic inflammation and cognitive impairments via mediating the gut-brain axis balance. J Agric Food Chem 16(68):14884–14895CrossRef

28.

Worby CJ, Schreiber HL, Straub TJ et al (2022) Longitudinal multi-omics analyses link gut microbiome dysbiosis with recurrent urinary tract infections in women. Nat Microbiol 7:630–639CrossRefPubMedPubMedCentral

29.

Stanley D, Mason LJ, Mackin KE et al (2016) Translocation and dissemination of commensal bacteria in post-stroke infection. Nat Med 22:1277–1284CrossRefPubMed

Titel: Gut microbiome in men with chronic prostatitis/chronic pelvic pain syndrome: profiling and its predictive significance
verfasst von: Shuai Wang
Maolin Zang
Xiaohui Yang
Linchen Lv
Lipeng Chen
Jianfeng Cui
Yaxiao Liu
Yangyang Xia
Nan Zhou
Zizhuo Yang
Yan Li
Benkang Shi
Publikationsdatum: 09.09.2023
Verlag: Springer Berlin Heidelberg
Erschienen in: World Journal of Urology / Ausgabe 11/2023
Print ISSN: 0724-4983
Elektronische ISSN: 1433-8726
DOI: https://doi.org/10.1007/s00345-023-04587-6

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Gut microbiome in men with chronic prostatitis/chronic pelvic pain syndrome: profiling and its predictive significance